Nested PCR for detection of mRNA transcripts:
Nested PCR Assay for Detection of Granulocytic Ehrlichiae
... specific nested PCR assay was developed for the detection of granulocytic ...the PCR assay using DNA extracted from whole blood as the template, compared with a serologic assay that identified ...
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Rapid Multiplex Nested PCR for Detection of Respiratory Viruses
... for detection by the “less sensitive” conventional methods (culture and ...multiplex nested PCR was observed, the differences were not statistically ...both PCR and conventional virus ...
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Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid
... Conclusions: Nested PCR is an effective way to detect trichomonads in bronchoalveolar lavage ...fluid, Nested PCR, Trichomonad, Lung Background Trichomonads are a type of parasitic flagellate ...
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Detection of Sporothrix schenckii in Clinical Samples by a Nested PCR Assay
... a nested PCR assay for the detection of ...and nested PCR but not from other microorganisms and human ...A detection limit of 40 fg of ...the nested PCR could ...
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A highly sensitive modified nested PCR to enhance case detection in leishmaniasis
... accurate detection of all light microscopy/in-vitro culture negative cases of all forms of leishmaniasis is necessary for ...a nested PCR (Mo-STNPCR) that also minimizes carry over and cross ...
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Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals
... respect, PCR is a poten- tial tool because of its high sensitivity and specificity in the diagnosis of schistosomiasis in humans ...parasite detection [24, 25]. The use of nested-PCR as a ...
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Single Tube Nested PCR for Detection of Tritrichomonas foetus in Feline Feces
... specific PCR test for the diagnosis of feline ...single-tube nested PCR was designed and optimized for the detection of ...The PCR is based on the amplification of a conserved portion ...
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Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR
... through detection of CDV from PBMC and body secretes of vaccinated ...two PCR methods with 51 PBMC samples suspected of CD, the efficiency of one-step RT-PCR com- bined with nested PCR ...
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Duplex Reverse Transcription PCR Followed by Nested PCR Assays for Detection and Identification of Brazilian Alphaviruses and Flaviviruses
... rapid detection and identification of Brazilian alphaviruses and ...genus-specific detection of Alphavirus and Flavivirus by a duplex reverse transcription-PCR (D-RT-PCR), followed by ...
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Colorimetric one tube nested PCR for detection of Trichomonas vaginalis in vaginal discharge
... one-tube nested PCR was developed for the detection of Trichomonas vaginalis in clinical vaginal discharge ...to nested PCR for semiquan- titative determination of ...parasites. ...
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Molecular Detection of Hepatitis C Virus (HCV) by Conventional One-step RT-PCR Coupled with Nested PCR
... by nested PCR. This method was based on two rounds of PCR amplification thereby increasing the detection time but the sensitivity and specificity of the assay was ...need nested types ...
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Comparison of PCR, Nested PCR, and Random Amplified Polymorphic DNA PCR for Detection and Typing of Ureaplasma urealyticum in Specimens from Pregnant Women
... A PCR assay, using three primer pairs, was developed for the detection of Ureaplasma urealyticum, parvo biovar, mba types 1, 3, and 6, in cultured clinical ...sensitive nested PCR was also ...
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Detection of Plasmodium using filter paper and nested PCR for patients with malaria in Sanliurfa, in Turkey
... Authors’ contributions AS, NYD, FYZ conceived and designed the study. NYD collected samples from participants. NYD performed microscopic examination. NYD extracted DNA from blood spotted filter papers. NYD carried out ...
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Detection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis
... for detection of fungal keratitis showed that 19 ...semi-nested PCR, and KOH; 11 patients tested positive using semi-nested PCR alone; and 21 patients tested negative for all 4 ...
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Multiplex nested PCR for detection of Xanthomonas axonopodis pv. allii from onion seeds
... Copyright © 2010, American Society for Microbiology. All Rights Reserved. Multiplex Nested PCR for Detection of Xanthomonas axonopodis pv. allii from Onion Seeds 䌤 † Isabelle Robe `ne-Soustrade,* ...
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Rapid Detection and Identification ofCandida, Aspergillus, and FusariumSpecies in Ocular Samples Using Nested PCR
... through PCR with sequence- specific primers is potentially more sensitive and rapid than microbiologic techniques, as a number of constraints are re- ...culture, PCR does not require the presence of viable ...
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Detection of Cryptosporidium and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism
... on nested PCR and restriction fragment length polymorphism (RFLP) that offers simple identification of Cryptosporidium hominis, Cryptosporidium parvum, and most other human infective species in stool ...for ...
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Detection of Epstein Barr Virus DNA in Thymic Epithelial Tumor Using Nested PCR
... H. Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ ...
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Molecular Detection of Adenoviruses in the Sinus Tissues of Patient by Nested-PCR in Shiraz, Southwest Iran
... rapid detection and accurate identification of pathogenic agents is needed for selecting an appropriate ...of nested-PCR method has indicated around ...
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Nested PCR for Rapid Detection of Mumps Virus in Cerebrospinal Fluid from Patients with Neurological Diseases
... virus PCR was evaluated with CSF from only six patients with CNS ...(RT)-nested PCR (n-PCR) procedure for the direct detection of mumps virus in CSF ...
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