Nested RT-PCR
Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex nested RT PCR
... nested RT-PCR revealed that ...rRNA PCR). Likewise, similar results were obtained when multiplex-nested RT-PCR was applied to the same cDNA samples ...single- ...
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Airborne Rhinovirus Detection and Effect of Ultraviolet Irradiation on Detection by a Semi-Nested RT-PCR Assay
... Prior to the aerosol experiments, we spiked filters with progressively more dilute viral suspensions to determine the limit of detection of our extraction and semi-nested RT-PCR assay. For spiking, 1 ...
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A general method for nested RT PCR amplification and sequencing the complete HCV genotype 1 open reading frame
... We optimized nested PCR conditions for each amplicon independently. The process is summarized in Figure 3. First, we designed primers for nested PCR. Because viral genetic heterogeneity will ...
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Evidence of West Nile virus infection in Nepal
... A total of 2,046 clinical specimens were collected from the fe- brile illness study at 4 hospitals in 3 cities in Nepal during May 2009 to December 2010. The patients presented with undifferentiated febrile illness with ...
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Molecular Detection of Hepatitis C Virus (HCV) by Conventional One-step RT-PCR Coupled with Nested PCR
... one-step RT-PCR assay followed by nested ...of PCR amplification thereby increasing the detection time but the sensitivity and specificity of the assay was ...need nested types of ...
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Crimean Congo Hemorrhagic Fever Virus: Genetic Analysis and Tick Survey in Turkey
... nucleotides [nt]) with the F2-R3/F3-R2 primer sets. Neither B. annulatus nor H. sulcata gave any positive nested-RT-PCR result against CCHFV (Table 2). The detection sensitivity of the assay was ...
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Truncated Particles Produced in Fish Surviving Infectious Hematopoietic Necrosis Virus Infection: Mediators of Persistence?
... The report of truncated particles in fish surviving an IHNV epizootic provided the first evidence that interference by viral DIPs might be the answer to a long-standing paradox in the life cycle of this virus (10). In ...
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are therefore assumed to be significant for the rapid diagnosis and identification of dengue ...
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Rearrangements of Rotavirus Genomic Segment 11 Are Generated during Acute Infection of Immunocompetent Children and Do Not Occur at Random
... two nested RT-PCR assays (termed A and B), using different combina- tions of back-to-back primer pairs (Table 1 and ...2), RT-PCR assays A and B gave concordant ...no PCR product ...
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Molecular Evolution Analysis and Geographic Investigation of Severe Acute Respiratory Syndrome Coronavirus-Like Virus in Palm Civets at an Animal Market and on Farms
... Both real-time and nested RT-PCR for the N and P genes showed that all of the palm civets and raccoon dogs sampled at the Xinyuan animal market prior to culling in January 2004 were posi[r] ...
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Evaluation of a Nested Reverse Transcription PCR Assay Based on the Nucleoprotein Gene for Diagnosis of Spontaneous and Experimental Bovine Respiratory Syncytial Virus Infections
... a nested RT- PCR whose target was the N gene to ensure high sensitivity and ...n RT-PCR-N to detect a very small amount of virus was demonstrated by the very low threshold of detection ...
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... real-time PCR to detect and quantitate viral load, and have shown its utility as an indicator of the extent of active infection ...by nested RT-PCR repre- sents the diagnostic method of choice ...
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Journal of Applied Pharmaceutical Science
... to nested RT- PCR for establishing the diagnosis of hepatitis C virus (HCV) in patients on ...& RT-PCR for ...or PCR 12 (57.14%) were positive for both ...
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Development of a Microplate Lectin Capture RT PCR (MLC RT PCR) for the Detection of Avian Infectious Bronchitis Virus
... MLC- RT-PCR, although the interactions of Con A with mo- lecular components of these viruses can not be ruled ...conventional RT-PCR, or with the virus isolation method in embryonated SPF ...
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A multiplex one tube nested real time RT PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
... multiple PCR and mono RT-qPCR assays have been routinely applied in the well-equipped laboratory for the diagnosis of RSV, HRV and ...Mono RT-qPCR [11, 12, 14] cannot simultan- eously detect RSV, HRV ...
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Optimization and validation of multi coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)
... When setting up the CE-based assay, the aim was to opti- mize throughput to reduce time and costs, as well as to simplify handling. This included (i) the use of 96-well plates in all consecutive steps from DNA extraction ...
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Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
... the nested PCR mix and 5–10 ng/µl to the TaqMan PCR mix, for samples isolated from spiked lake water, caused an increase in reaction sensitiv- ...strong PCR signals, but in the case of TaqMan ...
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Development of elisa and rt-pcr assays for rapid and sensitive detection of imperata yellow mottle virus infecting maize in burkina faso
... Imperata yellow mottle virus (IYMV) is an emerging pathogen infecting Imperatacylindrica and maize in Burkina Faso (West Africa). To study the epidemiology and characterize IYMV isolates, it is necessary to develop quick ...
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Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription PCR with an Armored RNA Internal Control
... (RT)- PCR may become the method of choice for the diagnosis of EV infections of the central nervous system, in many laboratories the diagnosis of EV infections still relies on cell culture tech- ...EV ...
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Serological and molecular detection of Toscana and other Phleboviruses in patients and sandflies in Tunisia
... using RT-PCR, we also identified the presence of SFSV in the CSF sample of one febrile pa- tient with meningoencephalitis from the Sousse region, situated in the lower semi-arid bioclimatic ...
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