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Next-generation sequencing platforms

Comparison of Sequence Reads Obtained from Three Next-Generation Sequencing Platforms

Comparison of Sequence Reads Obtained from Three Next-Generation Sequencing Platforms

... Next-generation sequencing technologies enable the rapid cost-effective production of sequence ...these sequencing technologies, investigation of the quality of sequence reads obtained from ...

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Towards a Next-Generation Sequencing Diagnostic Service for Tumour Genotyping: A Comparison of Panels and Platforms

Towards a Next-Generation Sequencing Diagnostic Service for Tumour Genotyping: A Comparison of Panels and Platforms

... perform sequencing of the entire cancer genome, our approach was to undertake targeted mutation ...two next-generation sequencing (NGS) platforms (Illumina MiSeq and Ion ...(MiSeq ...

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Comparison of Next-Generation Sequencing Panels and Platforms for Detection and Verification of Somatic Tumor Variants for Clinical Diagnostics

Comparison of Next-Generation Sequencing Panels and Platforms for Detection and Verification of Somatic Tumor Variants for Clinical Diagnostics

... of next-generation sequencing to detect somatic variants in DNA extracted from formalin-fixed, paraffin-embedded tumor tissues poses a challenge for clinical molecular diagnostic laboratories because ...

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Sequencing of BAC pools by different next generation sequencing platforms and strategies

Sequencing of BAC pools by different next generation sequencing platforms and strategies

... multiplex sequencing of BACs, particularly those with high repeat contents such as in barley, formation of chimeric contigs represents a major ...to sequencing, a process which is laborious and time ...BAC ...

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Evaluation of next generation sequencing platforms for population targeted sequencing studies

Evaluation of next generation sequencing platforms for population targeted sequencing studies

... con ends in the DNA samples after fragmentation prior to library generation. For the ABI SOLiD platform an amplicon end depletion protocol was employed to remove the overrep- resented amplicon ends; this was ...

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Assembly algorithms for next-generation sequencing data

Assembly algorithms for next-generation sequencing data

... algorithms Next-generation sequencing The emergence of next-generation sequencing platforms led to resurgence of research in whole-genome shotgun assembly algorithms and ...

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Next Generation Sequencing

Next Generation Sequencing

... Before addressing the technical details, we will outline some considerations regarding study design specific to NGS technology. There are 3 major manufacturers of the technology necessary to generate short reads, and ...

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Next Generation Sequencing

Next Generation Sequencing

... Next Generation Sequencing DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection Over the past three years, ...

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GeneSifter: Next Generation Data Management and Analysis for Next Generation Sequencing

GeneSifter: Next Generation Data Management and Analysis for Next Generation Sequencing

... NextGen Data Analysis Data analysis is of particular importance given that it has rapidly become the rate limiting step for many NextGen projects. At a very basic level, all NextGen platforms provide ...

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Next generation sequencing in thyroid cancer

Next generation sequencing in thyroid cancer

... multiple platforms and gene panels, and variable analysis programs, each of which can con- found ...new sequencing technologies, such as NGS, enhances the cancer genome body of knowl- edge, and allows for ...

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An Introduction to Next-Generation Sequencing Technology

An Introduction to Next-Generation Sequencing Technology

... Next-generation sequencing (NGS) platforms enable a wide variety of applications, allowing researchers to ask virtually any question of the genome, transcriptome, and epigenome of any ...

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Next-Generation Sequencing in Equine Genomics

Next-Generation Sequencing in Equine Genomics

... of sequencing technologies became a ...through platforms that allowed for multiple sequence reads to be collected at 1 time and also by coupling the inclusion of labeled nu- cleotides with the step of ...

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An Introduction to Next-Generation Sequencing Technology

An Introduction to Next-Generation Sequencing Technology

... Next-generation sequencing (NGS) platforms enable a wide variety of applications, allowing researchers to ask virtually any question of the genome, transcriptome, and epigenome of any ...

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What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research

What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research

... NGS platforms com- mercially available, the Genome Sequencer FLX from 454 Life Sciences ⁄ Roche, the Illumina Genome Analyser from Solexa and Applied Biosystems’ SOLiD (acronym for: ‘Sequencing by Oligo ...

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Application of Next-Generation Sequencing in Plant Breeding

Application of Next-Generation Sequencing in Plant Breeding

... decade, next-generation sequencing (NGS) platforms have changed the impact of sequencing on our knowledge of crop genomes and gene ...

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Application of next-generation sequencing technologies in virology

Application of next-generation sequencing technologies in virology

... cases sequencing occurs on a solid platform, whether a glass or microwell ...the platforms discussed above are producing smaller machines with such users in ...

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Next Generation Sequencing: Advances in Characterizing the Methylome

Next Generation Sequencing: Advances in Characterizing the Methylome

... bisulfite sequencing (RRBS) enriches for CpG rich regions of the genome without the need for a known set of targets ...each sequencing platform has unique ...Biosystems platforms produce short ...

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New Technology Development for Next-Generation Sequencing

New Technology Development for Next-Generation Sequencing

... CONCLUSION Next-Generation Sequencing (NGS) technologies are advancing at such a rapid pace that scientists are only beginning to access all the ways they may be ...

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Next generation sequencing

Next generation sequencing

... parallel sequencing: opportunities and challenges The multiple applications and uses of massively parallel sequencing are likely to reshape several aspects of breast cancer ...parallel sequencing of ...

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NEXT GENERATION SEQUENCING

NEXT GENERATION SEQUENCING

... ABNORMAL PAIRS SCANNER PREFILTERING ALGORITHM EXOME BUILDER EXOME DATASET ABNORMAL PAIRS HALF- MAPPED PAIRS PUTATIVE TRANSLOCATIONS SET (PTS) FILTERED HALF-MAPPED PAIRS FI[r] ...

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