Nile red
Predicting solvatochromic shifts and colours of a solvated organic dye : the example of Nile Red
... experimentally, nile red is observed to have a double peak structure in a number of non-polar solvents including n-hexane (see experimental spectra by Davis ...second, red-shifted peak predicted ...
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Nile Red Detection of Bacterial Hydrocarbons and Ketones in a High Throughput Format
... that Nile red is capable of detecting increased ketone production in vivo, Nile red staining of ketone-producing strains was used to gain information regarding the cellular localization of the ...
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The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae
... Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of ...
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Automation of a Nile red staining assay enables high throughput quantification of microalgal lipid production
... Results: Lipid production was monitored using the fluorescent dye Nile red with dimethyl sulfoxide as solvent facilitating dye permeation. The staining kinetics of cells at different concentrations and ...
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Nile Red Staining as a Subsidiary Method for Microplastic Quantification: A Comparison of Three Solvents and Factors Influencing Application Reliability
... were thoroughly stirred until no visible particles of Nile Red remained. Each sample was treated with 1 ml of the respective solution and allowed to rest for 48 h covered with a watch glass under a fume ...
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The effect of simvastatin on lipid droplets accumulation in human embryonic kidney cells and pancreatic cancer cells
... dyes, Nile Red (9-diethylamino- 5H-benzo[α]phenoxazine-5-one) and BODIPY® 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3α,4α-diaza-s- indacene) were used for lipid ...
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Dual-Modality, Dual-Functional Nanoprobes for Cellular and Molecular Imaging
... formulating Nile Red doped hexame- thyldisiloxane (HMDSO) nanoemulsions as dual modality (Magnetic Resonance Imag- ing/Fluorescence), dual-functional (oximetry/detection) nanoprobes for cellular and molec- ...
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A novel acemannan-chitosan modified lipid nanoparticles as intracellular delivery vehicles of antibiotic
... distributed red fluorescence intensity throughout the spherical NLC particles containing nile red fluorochrome ...the red fluorescence was concentrated in the middle of the particles and ...
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Contact-mediated intracellular delivery of hydrophobic drugs from polymeric nanoparticles
... from Nile Red was observed throughout the cytosol and Nile Red was located in spots resembling ...that Nile Red in the vesicular spots was binding to more lipophilic molecules ...
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Biocompatible nanocarriers that respond to oxidative environments via interactions between chitosan and multiple metal ions
... In conclusion, we have developed two series of biocompat- ible and oxidation-responsive NPs using the biopolymer CS and four different metal ions. The two series were produced with two different methods to combine the ...
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Journal of Applied Pharmaceutical Science
... Synthetic polymers are non-degradable and accumulated in the environment so, the efforts of scientists were forwarded to provide us with alternative environmentally biopolymers. Polyhydroxyalkanoates (PHAs) including ...
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Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii
... with Nile red and flow cytometry, a total of 76 viable mutants ...with Nile red and triple biological samples was essential to eliminate a substantial number of false positives (35 on 76, ...
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An inhibitor of oil body mobilization in Arabidopsis
... Figure 6: Diphenyl methylphosphonate prevents mobilization of pre existing lipid bodies. PTS1 GFP seedlings were grown on media containing either 0.1% DMSO (a) or 25 ,M diphenyl methylphosphonate (b) for seven days ...
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<p>Enhanced Chemotherapeutic Efficacy of PLGA-Encapsulated Epigallocatechin Gallate (EGCG) Against Human Lung Cancer</p>
... EGCG, (-)-epigallocatechin gallate; PLGA, poly(lactic-co- glycolic acid); SD, standard deviation; NPs, nanoparticles; NR, Nile Red; EE, encapsulation ef fi ciency; DL, drug loading; PDX, [r] ...
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A Comprehensive Study of High Cholesterol Diet-Induced Larval Zebrafish Model: A Short-Time In Vivo Screening Method for Non-Alcoholic Fatty Liver Disease Drugs
... Nile red is a lipophilic fluorescence material which can stain the TG and fatty ...0.5μg/ml Nile red or 10μM/L DCFH-DA (diluted with embryo water) for 30min at ...the Nile red ...
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The role of the hyaline spheres in sea cucumber metamorphosis: lipid storage via transport cells in the blastocoel
... Energetic lipid stores in A. mollis are primarily TAG, which are accumulated from the phytoplankton diet during the feeding larval stage for metamorphosis, as in other echinoderms (e.g. [3, 4, 37–39]). Our data suggest ...
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Constructing Empirical Likelihood Confidence Intervals for Medical Cost Data with Censored Observations
... It has been demonstrated by Adamo et al. that this bond rotation greatly increases the dipole moment across the entire molecule and that intramolecular charge transfer (ICT) is far more pro- nounced in this twisted ...
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Topical ocular delivery to laser-induced choroidal neovascularization by dual internalizing RGD and TAT peptide-modified nanoparticles
... stronger red fluorescence was exhibited around the CNV area in the TAT-Nile red-NP ...strongest Nile red accumulation was observed in the pattern of the iRGD and TAT dual-modified NPs ...
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Investigation into mycobacterial persistence and early markers of outcome in the treatment of pulmonary tuberculosis
... procedure was developed to remove extra-cellular lipid from the sputum matrix. The aim of lipase treatment was to remove lipids from the background matrix without degrading intra-cellular LBs. To confirm that LBs were ...
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Self-assembled mPEG–PCL-<em>g</em>–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro
... line was chosen to find the ratio suitable for gene transfec- tion, and then the B16-F10 cell line was chosen to test the gene delivery efficiency in cancer cells. A gene transfection efficiency of 60% ± 5% was achieved ...
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