Nitric oxide synthase 1
Activation of macrophage nuclear factor-κB and induction of inducible nitric oxide synthase by LPS
... The effect of LPS on the NF-κB signal tranduction pathway in alveolar macrophages was determined by EMSA. After treatment of macrophages with LPS for 30 min a substan- tially enhanced NF-κB binding complex was observed ...
6
Bacterial infection induces nitric oxide synthase in human neutrophils
... Leukocyte isolation and immunocytochemistry. Cell pellets from fresh nitrite positive, leukocyte esterase positive urines were incu- bated with CD45 (Anti-HLe-1) monoclonal antibody or with CD14 (LEU-M3) ...
8
Endothelial nitric oxide synthase G894T polymorphism increases the risk of infertility in women with endometriosis
... MboI (10 U/µL, Promega, USA), 2 µg acetylated BSA (10 µg/µL, provided with the restriction enzyme) to a final digestion volume of 30 µL. Digestion was run in a digital thermomixer (Vortemp 56, Labnet Inc, USA) at 400 rpm ...
10
Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells
... Parkia speciosa Hassk, locally known as petai papan, is a common medicinal plant found in Southeast Asia. Its empty pods were reported to contain high concentrations of polyphenols, particularly quercetin. This study ...
7
Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages
... inducible nitric oxide synthase (iNOS) forward and reverse primers: 5′- CAC CTT GGA GTT CAC CCA GT -3′ and 5′- ACC ACT CGT ACT TGG GAT GC -3′; interleukin-1 β (IL-1 β ) forward and ...
9
Urinary Magnesium and Calcium Excretion after Neuronal Nitric Oxide Synthase Inhibition in Normotensive and Spontaneously Hypertensive Rats.
... Experiments were carried out on conscious, male, normotensive Wistar rats (n=10) and spontaneously hypertensive rats (SHR, n=10) at the same age of 12-14 weeks. In the SHR group were included animals with systolic blood ...
7
Nitrite Derived from Endogenous Bacterial Nitric Oxide Synthase Activity Promotes Aerobic Respiration
... excluding a role for nitrate as the modulator of growth (Fig. 3D and E). As expected, nitrite was effectively able to rescue the growth defect associated with the narA nos double mutant (Fig. 3F). However, since nitrite ...
16
Signalling pathway involved in nitric oxide synthase type II activation in chondrocytes: synergistic effect of leptin with interleukin 1
... fibrillation). For chondrocyte isolation, aseptically dissected cartilage was subjected to sequential digestion with pronase (catalogue number 165921; Roche Molecular Biochemicals, Indianapolis, IN, USA) and collagenase ...
11
TLR2 signal influences the iNOS/NO responses and worm development in C57BL/6J mice infected with Clonorchis sinensis
... 5]. Nitric oxide (NO) formation and nitrosation may contribute to the development of liver fluke-associated carcinogenesis ...by nitric oxide synthase ...
10
The caveolar nitric oxide synthase/arginine regeneration system for NO production in endothelial cells
... than decreased, and there was no apparent change in methylarginine levels. To further examine the molecular basis for the stimulation of NO production by citrulline, we compared the effect of exogenous citrulline with ...
5
A Critical Role of Nitric Oxide in Human Immunodeficiency Virus Type 1-Induced Hyperresponsiveness of Cultured Monocytes
... Recently, we demonstrated both induction of nitric oxide synthase (NOS) in and production of nitric oxide (NO) by HIV-1-infected mono-.. cytes (10).[r] ...
9
Changes in the nitric oxide system in the shore crab Hemigrapsus sanguineus (Crustacea, decapoda) CNS induced by a nociceptive stimulus
... inducible nitric oxide synthase (iNOS)-immunohistochemistry and immunoblotting, we characterized the nitric oxide (NO)-producing neurons in the brain and thoracic ganglion of a shore ...
9
Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells
... The rat basophilic leukemia cell line, RBL-2H3 (ATCC, American Type Culture Collection, Rockville, MD, USA) was grown in complete Eagle Minimal Essential Medium with 15% fetal bovine serum (FBS), 0.1 mM non-essential ...
9
A CLINICAL COMPARATIVE STUDY TO EVALUATE THE EFFICACY AND SAFETY OF LIV 52 DS TABLETS IN NONALCOHOLIC STEATOHEPATITIS (NASH)
... Hepatic steatosis with or without hepatitis, in the absence of alcohol use, was first described by Ludwig et al. and is referred to as non-alcoholic steatosis or Non-Alcoholic Steatohepatitis. [1] Non-Alcoholic ...
17
Nitric oxide synthase and the contribution of nitric oxide to vertebrate motor control
... the role of NO in the ‘metamodulation’ of locomotion. I have already delved into the characteristics of this messenger molecule earlier in this introduction, but now I will describe som[r] ...
153
Role of nitric oxide in the gastrointestinal tract
... Worldwide osteoarthritis (OA) affects more than 9.6% of men and 18% of women older that 60 years. Treatment for OA often requires chronic use of selective or nonselective nonsteroidal anti- inflammatory drugs (NSAIDs), ...
6
Effect of L- Arginine, the Nitric Oxide Precursor, on Morphine Tolerance in Ovariectomized Rats
... Estrogen has been shown to influence the NO system, it increases NO production (18,19), endothelial nitric oxide synthase (eNOS) expression (18,20), and eNOS activity (18,19) in cultured endothelial ...
12
Serotonin and platelet studies in vascular disease
... One of the objectives of this thesis was to deve l o p a rapid, sensitive, method to measure PSC. A p a r t i c l e counter (Coulter ZM) coupled to a channelyzer (Coulter C-256) was selected. This apparatus provides at ...
351
Research Paper: Correlation Between Serum and Seminal Leptin and Nitric Oxide (Nitrite) in Asthenote-ratozoospermic Males
... nfertility is among the most serious health problems worldwide. The prevalence of in- fertility is growing. One out of every 6 to 7 couples experience difficulties with concep- tion, globally [1]. Infertility ...
8
Pulsed low frequency magnetic field on obese subjects
... inducible nitric oxide synthase, endothelial nitric oxide synthase and cyclooxygenase-2 in the human nocyte cell line HaCat: potential therapeutic effects in Department of Drug ...
5