NK-92
Increase of IFN-γ and TNF-γ production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor
... [37]. NK cells comprise the major cells involved in early responses against infected and tumor cells ...of NK cells and CD8+ T cells, having been adopted as a marker of activation due to the association ...
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<p>DNAM1 and 2B4 Costimulatory Domains Enhance the Cytotoxicity of Anti-GPC3 Chimeric Antigen Receptor-Modified Natural Killer Cells Against Hepatocellular Cancer Cells in vitro</p>
... (both NK-cell-associated costimulatory domains). Respective CAR-NK-92 cells were ...Results: NK-cell-associated costimulatory signal was necessary for CAR-NK-92 ...CAR- ...
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A combinational therapy of EGFR-CAR NK cells and oncolytic herpes simplex virus 1 for breast cancer brain metastases
... Previous data from our group and others demonstrated that oHSV-1 can lyse glioblastoma cells but spare normal cells [11, 14, 15]. In the current study, we explored whether oHSV-1 alone could lyse and destroy breast ...
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<p>Forskolin exerts anticancer roles in non-Hodgkin’s lymphomas via regulating Axin/β-catenin signaling pathway</p>
... and nK-92 cells were examined by Western blotting analysis (n=3, ...or nK-92 cells were transfected with si-1/2/3 targeting axin gene for 24 or 48 hours, respectively (n=3, ...and ...
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A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling
... [12]. Pneumatic valves were integrated into the chip to enable the rapid and repeated fluid exchange. Cells were mechanically captured in hydrodynamic traps and isolated in separate microchambers of about 70 pL in volume ...
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IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma
... in NK‑92 cells. a NK‑92 cells transduced with LMP1‑encoding lentivirus led to higher levels of p‑Raf‑B, p‑p38, p‑JNK, p‑ERK, and p65 than transduction with NC ...b–f NK‑92 cells ...
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Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells
... to NK cells but silencing the other 2 members of this family (JAK3 and TYK2) did not have any ...or NK-92 effector ...to NK cell ...with NK cell recognition and activa- tion (22–24), ...
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Purinergic targeting enhances immunotherapy of CD73+ solid tumors with piggyBac-engineered chimeric antigen receptor natural killer cells
... improve NK cell kill- ing against CD73 + ovarian cancer cells in vitro by acting on mechanisms that may be ascribed to both ADCC and reduced adenosine levels ...or NK cells in the early stages of tumor ...
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An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 allele
... The NK-92 cell line, derived from a lymphoma patient, has previously been well characterized and adoptive transfer of irradiated NK-92 cells has demonstrated safety and shown preliminary ...
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Novel anti-CD3 chimeric antigen receptor targeting of aggressive T cell malignancies
... of NK CAR therapy are a short NK cell half-life and thus transient, defined CD3CAR activity ...CD3CAR NK-92 dose-dependent tumorlysis in T-ALL cell lines and CD3+ leukemic patient ...CD3CAR ...
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Human immunodeficiency causing mutation defines CD16 in spontaneous NK cell cytotoxicity
... both NK cell lines, and the presence of CD16 did not lead to sig- nificant differences at any individual time point or over the entire time course (Figure ...
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Herpes virus ICP47 and US11 suppression of CTL activity adapted for use in gene therapy
... Both LISN and LUSN function to decrease class I MHC cell surface expression in the TALL-104 and NK-92 lymphocyte cell lines.. NK-92 LEL cells transduced with LXSN, LISN, and LUSN vectors[r] ...
147
Design and analysis of stably integrated reporters for inducible transgene expression in human T cells and CAR NK-cell lines
... of NK cell lines appears somewhat surprising given the well-established positive role of NFAT in T- and B-cell activation, yet this was not unex- ...for NK cell ac- tivation and is furthermore associated ...
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Adipose cells promote resistance of breast cancer cells to trastuzumab mediated antibody dependent cellular cytotoxicity
... Human mammary epithelial hTERT-HME1 and hMADS cells were seeded at 1.2 × 10 5 cells/ml/well in 12-well plates. The medium of 12-day #hMADS was changed and supplemented with 10% FCS. Two days later, target cells were ...
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Natural Born Killers: NK Cells in Cancer Therapy
... constructed NK-92 cells expressing a modified CAR consisting of extracellular TGFRII fused to the intracellular signaling domain of the activating receptor, NKG2D (TGFRII-NKG2D, termed ...
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Interactions between Siglec 7/9 receptors and ligands influence NK cell–dependent tumor immunosurveillance
... human NK cells that recognize sialic acid–containing ...cytotoxic NK cells with a mature phenotype and enhanced chemotactic ...+ NK cell population was reduced in the peripheral blood of cancer ...of ...
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Split anergized natural killer cells halt inflammation by inducing stem cell differentiation, resistance to NK cell cytotoxicity and prevention of cytokine and chemokine secretion
... anergized NK cells, we determined NK cell mediated cytotoxicity when OSCSCs were treated with supernatants of NK cells treated with anti-CD16mAb and IL-2 in the presence and absence of each of IFN-γ ...
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Determination of natural killer cell function by flow cytometry.
... for NK cell activity has been the chromium release ...of NK cell ...for NK activity in healthy adults and identified a small cluster of individuals who have exceptionally high or low levels of ...
6
Aggressive mature natural killer cell neoplasms: from epidemiology todiagnosis
... Mature NK cell neoplasms comprise a wide spectrum of entities, from cases with an indolent disease course (chronic NK-cell lymphocytosis) to cases with aggressive clinical behavior (NKTCL and ...Aggressive ...
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NK cell compartment in patients with coronary heart disease
... elderly, NK cells constitute the main mechanism of antiviral defense ...affects NK cell functions; our pre- vious studies showed that high concentration of proin- flammatory cytokines correlates with low ...
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