Non-viral gene vector
Improved osteogenic vector for non-viral gene therapy
... of gene therapy encoding an exogenous gene on a plasmid (Feichtinger et ...alternative, gene therapy allows local and sustained release of growth ...of gene therapy in order to stimulate bone ...
14
Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
... favorable non-viral vectors ...efficient gene transfection into cell while causing low toxicity, genetically manipulations of non- viral vector, cationic porphyrin, would be ...
32
Non viral gene delivery systems for tissue repair and regeneration
... PEG-based vector complexes into collagen scaffolds to examine their effects on ECM remodelling following cutaneous ...sient gene expression by PLL-g-PEG polymer-mediated pDNA [encoding a truncated form of ...
20
Cationic lipids and investigations into non-viral gene delivery systems
... adenoviral gene therapy in clinical trials highlights the potential dangers of gene therapy if the vector chosen is unsuitable for the patients n e e d s/F u rth e rm o re , gene therapy ...
267
Targeting MicroRNAs in Cancer Gene Therapy
... replicating viral vector to mediate the expression of an artificially designed interfering long non-coding RNA (lncRNA), which comprises the binding sites of multiple oncogenic miRNAs and thus ...
15
An MRI-visible non-viral vector for targeted Bcl-2 siRNA delivery to neuroblastoma
... polymers such as branch polyethylenimine (BPEI, 25 kDa), have been frequently used because they offer significantly more protection against nuclease degradation. However, the large amount of positive charge results in ...
14
Changing Viral Tropism Using Immunoliposomes Alters the Stability of Gene Expression: Implications for Viral Vector Design
... Many strategies for redirecting the tropism of murine Moloney leukemia virus (MMLV) have been described. Preformed virion- liposome complexes, termed virosomes, have been reported to be relatively stable. Virosomes ...
11
Mesenchymal stem cell fate following non viral gene transfection strongly depends on the choice of delivery vector
... Traditionally, viral vectors such as retrovirus, lentivirus and adenovirus, have been used for the delivery of genes into cells via a process known as transduction ...stable gene expression, many ...
30
Gene therapy for the treatment of cystic fibrosis
... available gene transfer agent that could be used in a trial designed to assess clinical ...a non-viral gene transfer system was ...optimal non-viral vector was found to be ...
8
Non-viral eNOS gene delivery and transfection with stents for the treatment of restenosis
... a viral vector that is associated with a polymer coating via an antibody ...any vector must be exceptionally ...eNOS gene therapy using adenoviral vector embedded in stents for the ...
14
NANO-STRUCTURES FOR NON-VIRAL GENE DELIVERY FOR CANCER THERAPY: A REVIEW
... this vector is used for in vitro and in vivo gene ...successful gene transfer of an antisense glutamic acid decarboxylase, mRNA expression plasmid using a PLL-PEG ...
7
In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum
... HSV-1 vector can clearly be used to introduce genes into different regions of the mature mammali- an brain in vivo [20], with successful transfection of HSV-1 viral vector into the rat basal ...
9
Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System
... while non-viral gene delivery techniques work toward efficiency of DNA delivery, they generally prove poor in the delivery of pDNA vectors to the nuclear com- ...of non-viral ...
13
Accuracy estimation of foamy virus genome copying
... pKG83 vector and the three packaging plasmids pCZIgag-2, pCZIpol, and pCZenvEM002 [71,73] or with ...μg vector and the packaging plasmids plus the ...of viral transcription by the addi- tion of 10 mM ...
15
Hermes, a Functional Non-Drosophilid Insect Gene Vector From Musca domestica
... Al- though transgenic non-drosophilid insects and mam- malian cells were occasionally recovered using P- element-containing vectors, none resulted from trans- positiona[r] ...
8
Multivalent and Multipathogen Viral Vector Vaccines
... Viral vector vaccines have been applied extensively in veterinary medicine (although these will not be discussed in detail in this ...(VacV) vector vaccine expressing the glycoprotein (GP) of ...
15
Intragenic transcriptional cis activation of the human immunodeficiency virus 1 does not result in allele specific inhibition of the endogenous gene
... Integration site selection by retroviruses is not sequence- specific but also not random. HIV-1 favors integration within active transcription units [6-8]. Additional features are the requirement of host factors such as ...
15
A sight on the current nanoparticle based gene delivery vectors
... potential vector to deliver genes. They are used in gene therapy for tumor and other miRNA-related diseases such as diabetes and cardiovascular and neurodegenerative ...
9
Construction of Retroviral Vectors with Improved Safety, Gene Expression, and Versatility
... of gene expression from several different types of retroviral vectors currently used in gene therapy clinical trials ...retroviral vector was superior in con- ferring gene expression after ...
11
Epstein-Barr Virus Late Gene Transcription Depends on the Assembly of a Virus-Specific Preinitiation Complex
... late gene transcription by remodeling the chromatin structure of the viral ...for gene expression ...episomic viral genome, the newly synthesized DNA is probably not covered by nucleosomes ...
14