Nonidet P-40
Properties of a Ribonucleoprotein Particle Isolated from Nonidet P-40-Treated Rous Sarcoma Virus
... RNA was isolated from purified RSV, RNP, and soluble fraction by dissociating it from other virion components in 0.1% w/v SDS at room temperature, Downloaded from http://jvi.asm.org/ on [r] ...
11
Insertions within the hepatitis B virus capsid protein influence capsid formation and RNA encapsidation.
... PBS–1% Nonidet P-40, and the labeled core protein was immunoprecipitated with a mixture of mouse anti-core protein and anti-precore protein sera made against insect cell-derived HBV core and precore ...
6
Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus.
... Treatment of IBV with Nonidet P-40 followed by sedimentation on sucrose density gradients produced virus particles with coronas of surface projections into which the stain had penetrated[r] ...
5
Monoclonal antibodies against baboon endogenous virus and against host cell antigens.
... Antibody binding assay in culture fluids from cloned hybrid cell lines 12-I-labeled protein A cpm bound on: Clone A24cls A204-M7 A204 cells' M7 viral antigen ceilsa Nonidet P-40, 2 x 10-[r] ...
9
A mutant of human immunodeficiency virus with reduced RNA packaging and abnormal particle morphology.
... Viral particles harvested during an acute infection of A3.01 cells with wild-type pNL4-3 or mutant pNLW1 virus were pelleted at 105 x g and lysed in 0.5% Nonidet P-40.. Serial fourfold d[r] ...
5
T-cell receptors from virus-specific cytotoxic T lymphocytes recognizing a single immunodominant nine-amino-acid viral epitope show marked diversity.
... Cytoplasmic RNA was extracted from CTL clones by using Nonidet P-40-sodium dodecyl 352 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Following infection of the H-2d mo[r] ...
6
Retrovirus protease characterized as a dimeric aspartic proteinase.
... The assay for retrovirus protease activity was performed in a buffer containing 20 mM PIPES [piperazine-N,N'-bis2ethanesulfonic acid] pH 6.5, 1 mM dithiothreitol, and 0.5% Nonidet P-40 w[r] ...
7
Characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus.
... DISCUSSION Dissociation of purified LCM virus by treatment with Nonidet P-40 in 0.5 M KCl and sedimentation analysis in sucrose gradient containing 0.3 M KCl led to the isolation of ribo[r] ...
8
In vitro proteolytic cleavage of Gazdar murine sarcoma virus p65gag.
... 963 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Moloney murine leukemia virus, disrupted in concentrations of 0.1 to 0.5% Nonidet P-40, catalyzed the cleavage of p6[r] ...
5
The cellular proteins which can associate specifically with polyomavirus middle T antigen in human 293 cells include the major human 70-kilodalton heat shock proteins.
... To determine whether any of the human MTAPs were major cellular proteins, we analyzed Nonidet P-40 lysates of 293 cells metabolically labeled with methionine in parallel with MT immunopr[r] ...
7
Two forms of VPg on poliovirus RNAs.
... The cytoplasm was obtained from infected cells lysed with 1% Nonidet P-40, and cytoplasmic RNA was extracted with phenol as described 27, except that the cell suspension was made 863 Dow[r] ...
9
94,000- and 100,000-molecular-weight simian virus 40 T-antigens are associated with the nuclear matrix in transformed and revertant mouse cells.
... The complete extraction of T-ag from the nucleus requires Nonidet P-40 detergent NP-40 in a buffer of pH 8.0 or higher, since not all T-ag is removed at pH The nucleus of eucaryotic cell[r] ...
9
Microscopic structure of coexisting 0+ states in Ni 68 probed via two-neutron transfer
... states and are shown in Fig. 3 together with two-step distorted-wave Born approximation (DWBA) calculations performed with the F RESCO code [32] including both direct and sequential transfer. Solid red lines correspond ...
7
Overview of hard hadron production results in ALICE
... A review of recent ALICE results on hard hadron and jet production in pp, p-A and A-A collisions was presented. Spectra of unidentified charged particles and of identified hadrons were measured in pp collisions at ...
12
Fruit and vegetable statistics for 1966/67. Information Memo P-40/68, June 1968
... a9 fnrrits, nais il Alt toutefois renarquer que le rythrne d?accroisgement de ees ventes i lr6tranger est beaucoup plus sp6ctaculaife que oeLui cl.e ses achats, Li enoore le progrbs d.es[r] ...
8
Exploring legitimacy in major public procurement projects
... ‘..respond most effectively and efficiently to the community’s needs..’ P.9 ‘..we want the Fire Service to be an effective, responsive community service.’ P.40 ‘Unless it is repealed, th[r] ...
37
Volume 40 - Article 40 | Pages 1153–1166
... To encourage and facilitate the use of this data, we provide a single, standardized, flat data file containing county-to-county one-year migration flows for the period 1990–2010 (contain[r] ...
16
Assessment for learning
... afl_txt_final 24/3/05 7:40 am Page 35 The New Heys model : a case study 35 Benefits to learners : p empowerment p increased ownership of their learning p greater understanding of the lea[r] ...
49
Sevoflurane reduces clinical disease in a mouse model of multiple sclerosis
... mice, 40 minutes with sevoflurane increased the total number of CD4+ lymphocytes in the spleen [12]; and in vitro sevoflurane increased P-selectin ex- pression and platelet:leukocyte adherence in whole ...
10
European Social Fund - annual report 1980. Information Memo P-40/81, July 1981
... SociaI Fund response The Sociat Fund commitments budget in 1980 was 909.5 MECU and a carry-over of 11?.4 ttlECU f rom the previous year brought totaI resources avai Labl.e in 1980 up to'[r] ...
6