nonsense-mediated RNA decay
Control of gene expression through the nonsense-mediated RNA decay pathway
... Nonsense-mediated RNA decay, initially discovered as a quality control mechanism that targeted mutant tran- scripts for degradation, is now widely appreciated as a key mechanism that regulates ...
12
The nonsense mediated RNA decay pathway is disrupted in inflammatory myofibroblastic tumors
... Inflammatory myofibroblastic tumors (IMTs) are characterized by myofibroblast proliferation and an inflammatory cell infiltrate. Little is known about the molecular pathways that precipitate IMT formation. Here, we ...
6
Gene Set Coregulated by the Saccharomyces cerevisiae Nonsense-Mediated mRNA Decay Pathway
... partially mediated by the tyrosine kinase Swe1, a negative regulator of the Cdc28-Clb complexes ...of RNA polymerase ...of RNA polymerase ...of RNA polymerase II was effective in these ...
12
The Human T-Lymphotropic Virus Type 1 Tax Protein Inhibits Nonsense-Mediated mRNA Decay by Interacting with INT6/EIF3E and UPF1
... in RNA stability are an important source of gene expression altera- tions in stressed cells (20), future systematic studies of NMD- sensitive transcripts increased by Tax should help to define more precisely how ...
14
UPF2, a nonsense mediated mRNA decay factor, is required for prepubertal Sertoli cell development and male fertility by ensuring fidelity of the transcriptome
... in RNA processing, RNA splicing or cellular homeostasis (Table 1; supplementary material Table S1), suggesting that Upf2 inactivation disrupts the RNA-processing ...of RNA processing- related ...
11
Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations
... by nonsense mutations, we first con- ducted dose-response experiments in normal mice with ASOs targeting mRNAs encoding each of the ten NMD fac- ...the RNA and protein levels (Additional file 2: Table S1 ...
16
Nonsense mediated mRNA decay affects nonsense transcript levels and governs response of cystic fibrosis patients to gentamicin
... obtained RNA samples from nasal epithelial cells from patients who had participated in our double-blind, placebo-controlled, crossover trial ...of nonsense CFTR mRNA was analyzed in pretreatment samples ...
11
Multiple Nonsense-Mediated mRNA Processes Require Smg5 in Drosophila
... The nonsense-mediated messenger RNA (mRNA) decay (NMD) pathway is a cellular quality control and post-transcriptional gene regulatory mechanism and is essential for viability in most ...
12
Conservation of Nonsense-Mediated mRNA Decay Complex Components Throughout Eukaryotic Evolution
... Here we take a genomic approach to provide a framework to interpret the functional data from different species and to understand the origins, mechanism and evolution of NMD. By mapping the distribution of SURF complex ...
12
TRPC1 transcript variants, inefficient nonsense-mediated decay and low up-frameshift-1 in vascular smooth muscle cells
... Nonsense-mediated decay (NMD) is a major RNA surveillance mechanism, degrading mRNAs that contain premature termination codons (PTCs) in eukaryotic cells ...RENT1). Decay of ...
10
Integration of splicing, transport and translation to achieve mRNA quality control by the nonsense mediated decay pathway
... mRNA decay (NMD) is a specific RNA- degradation process by which eukaryotic cells can eliminate mRNAs that contain premature translation-termination codons ...
6
SR proteins and the nonsense mediated decay mechanism are involved in human GLB1 gene alternative splicing
... multifunctional splicing factors required both for spliceo- some assembly and for alternative splicing [6]. On the other hand, the best characterized ESSs and ISSs are recog- nized by members of the heterogeneous nuclear ...
9
A role for nonsense mediated mRNA decay in plants : pathogen responses are induced in Arabidopsis thaliana NMD mutants
... Total RNA was extracted from leaves or whole plants using the QIAGEN RNeasy kit as per manufacturer’s instructions, including the optional on-column digestion of ...DNA-free RNA were reverse transcribed ...
14
A Role for the Nonsense-Mediated mRNA Decay Pathway in Maintaining Genome Stability in Caenorhabditis elegans
... containing RNA (TERRA) needed for the regulation of telomerase activity (Azzalin et ...an RNA- and DNA-dependent 59-3 helicase activated by hSMG1 phosphorylation (He and Jacobson ...an RNA, DNA, or a ...
12
Synthesis and activity of a novel inhibitor of nonsense-mediated mRNA decay
... spliced RNA and not unspliced pre-mRNA, the amplified PCR products from RNA extracted from cells transfected with either NORM, or TER, were examined by DNA agarose gel elec- trophoresis ...
5
Nonsense-Mediated mRNA Decay Mutation in Aspergillus nidulans
... total RNA was reverse transcribed from random hexamer primers by using SuperScript II reverse transcriptase (Invitrogen, Pais- ley, Scotland, United Kingdom) according to the manufacturer’s ...total RNA ...
9
Nuclear Import of Upf3p Is Mediated by Importin-α/-β and Export to the Cytoplasm Is Required for a Functional Nonsense-Mediated mRNA Decay Pathway in Yeast
... Figure 4.—Effects of Upf 2p overexpression in transformants expressing upf 3-Triple-HA and upf3- Triple-HA-Rev. (A) Suppression of leu2-1, tyr7-1, and can1-100 was as- sayed by serial drop tests as de- scribed in Figure ...
18
Regulation of Natural mRNAs by the Nonsense-Mediated mRNA Decay Pathway
... An upstream open reading frame, or uORF, is an open reading frame that is located in the 5= UTR of a transcript (Fig. 1C). mRNAs can have one or more uORFs, some of which overlap the main protein coding region. These ...
10
Human alternative Klotho mRNA is a nonsense-mediated mRNA decay target inefficiently spliced in renal disease
... in RNA surveillance reveal that premature termination codons, as present in alternative Klotho mRNA (for secreted Klotho), prime mRNAs for degradation by nonsense-mediated mRNA decay ...
16
Assessing the activity of nonsense-mediated mRNA decay in lung cancer
... of nonsense-mediated mRNA decay (NMD) in tumor cells can suppress tumor growth through expressing new antigens whose mRNAs otherwise are degraded by ...using RNA-seq data to measure NMD ...
7