Normal human skin fibroblasts
Prostaglandin E2 induces Skin Aging via E-prostanoid 1 in Normal Human Dermal Fibroblasts
... fragile skin, with increased susceptibility to bruising and impaired wound healing ...In human skin, collagen fibers account for ~75% of the dry weight of the dermis, while collagen type I makes up ...
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Synthesis and antimicrobial activity of chiral quaternary <em>N</em>-spiro ammonium bromides with 3',4'-dihydro-1'H-spiro[isoindoline-2,2'-isoquinoline] skeleton
... Our in vitro data confirmed that all tested compounds do not possess cytotoxic and proapoptotic potency in normal cells such as human skin fibroblasts. Our study needs further examination to ...
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Attenuated prostaglandin formation in peroxisomal deficient human skin fibroblasts
... Zellweger's fibroblasts were incubated with arachidonic ...Zellweger's fibroblasts with media containing arachidonic acid, and it was not due to channeling of arachidonic acid into other eicosanoid ...
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Original Article Association of CD14 and TLR4 with LPS-stimulated human normal skin fibroblasts in immunophenotype changes and secretion of TGF-β1 and IFN-γ
... Using transmission electron microscope, we fo- und diverse morphologies, high ratio of nucleo- plasm, irregular nuclear membrane, and a large number of rough endoplasmic reticulums, Golgi complexes, microtubules and ...
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n Butyrate inhibition of hyaluronate synthesis in cultured human fibroblasts
... cultured human skin fibroblasts. Normal fibroblast cultures were grown to confluence, shifted to a medium without or with n-butyrate for 24 h, labeled with either [3H]acetate or ...
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In vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold
... designing skin replacements, the goal has been to re-create this model and make a product which has both essential ...cultured human keratinocytes on the surface of modified collagen-chitosan scaffold that ...
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Original Article Role of thrombospondin-4 in fibroblasts from normal skin and hypertrophic scars
... in fibroblasts from normal skin and hypertrophic ...Methods: Human normal skin-derived and hypertrophic scar-derived fibroblasts were cultured, and TSP4 gene expression ...
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Selective expression of a normal action of the 1,25 dihydroxyvitamin D3 receptor in human skin fibroblasts with hereditary severe defects in multiple actions of that receptor
... were normal for antimitogenic action of ...was normal in EC50 and normal or increased in maximal ...in normal cells showed a similar profile of potency ratios for 1,25-(OH)2D3 and six ...a ...
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Sialylation regulates myofibroblast differentiation of human skin fibroblasts
... Cells were fixed with 4% (w/v) paraformaldehyde and washed. Next, cells were permeabilized and blocked with PBS containing 0.2% (v/v) Triton™ X-100, 1% (w/v) BSA, and 5% (v/v) normal goat serum. After washing, ...
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Reduced affinity of the androgen receptor for 5 alpha dihydrotestosterone but not methyltrienolone in a form of partial androgen resistance Studies on cultured genital skin fibroblasts
... We have studied a child with posterior labial fusion, clitoral phallus, female urethra, and a short, blind vagina born to a mother with decreased axillary and pubic hair. Her karyotype is 46,XY. At 2 yr of age, the ...
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Proliferation and Differentiation of Human Hair Follicle Stem Cells on Chitosan-Skin Engineered Template in Vitro
... chitosan skin-engineered templates (SETs) as scaffolds for the proliferation of human fibroblasts have shown the promise of SETs in facilitating skin cell growth in three-dimensional ...using ...
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Transcription Abnormalities Potentiate Apoptosis of Normal Human Fibroblasts
... response to transcription inhibitors and cell death is associated with prolonged induction of p53, as would be expected for p53 monitoring of correct transcription that is required for n[r] ...
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Metabolism of testosterone 14C by cultured human cells
... to normal age and sexmatched controls. Fibroblasts of children with testicular feminization metabolized testosterone predominantly through the 17-ketonic pathway and manifested a pattern of testosterone ...
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Adipose tissue-derived extracellular fraction characterization: biological and clinical considerations in regenerative medicine
... Recently published studies have demonstrated that ADSCs produce complementary wound healing trophic factors in- cluding insulin-like growth factor (IGF), bFGF, HGF, VEGF, KGF, and Wnt10b [41, 42]. A number of these ...
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CHILD syndrome Phenotypic dichotomy in eicosanoid metabolism and proliferative rates among cultured dermal fibroblasts
... Dermal fibroblasts from a patient with CHILD syndrome (an acronym for congenital hemidysplasia with ichthyosiform erythroderma and limb defects) were obtained and successfully maintained in ...culture. ...
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Protective influence of hyaluronic acid on focal adhesion kinase activity in human skin fibroblasts exposed to ethanol
... groups. Human skin fibroblasts were incubated for 24 hours with different concentrations of ethanol when HA was added, and then washed three times with ...of human skin fibro- blasts ...
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Role of superoxide anion in host cell injury induced by mycoplasma pneumoniae infection A study in normal and trisomy 21 cells
... epithelial cells decreased by 74-77% as compared with uninfected controls. Addition of superoxide dismutase to the infected cultured cells totally prevented the inhibition whereas addition of catalase or catalytically ...
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Endothelin and oxytocin induced calcium signaling in cultured human myometrial cells
... in human amniotic fluid, suggests that the myometrium may be an important target organ for this ...quiescent human myometrial cells ET produced a dose-dependent increase in cytosolic free Ca2+ (Cai2+), ...
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Herpes simplex virus glycoproteins E and I facilitate cell-to-cell spread in vivo and across junctions of cultured cells.
... Replication of gE- and gI - mutant viruses in human fibroblasts were normal, and the rates of entry of mutant and wild-type viruses into fibroblasts were similar; however, spread of gE -[r] ...
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Replication of simian virus 40 DNA in normal human fibroblasts and in fibroblasts from xeroderma pigmentosum.
... The studies described above indicated that the semipermissive nature of infection of early passage human fibroblasts with SV40 is consistent with a readily detectable level of viral DNA [r] ...
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