Northern blotting
A non-radioactive method for small RNA detection by northern blotting
... Because northern blots performed using biotin-labeled probes were sensitive and efficient, we used this method to confirm the expression of classic miRNAs and siR- NAs in ...before northern blotting ...
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U1snRNP mediated suppression of polyadenylation in conjunction with the RNA structure controls poly (A) site selection in foamy viruses
... context. Northern blotting analysis of RNA from BHK-21 cells co-transfected with either pHSRV13 or pHSRV13SDm2, and wild-type or SDm2 U1snRNA expression ...
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Transcriptional Analysis of Latent and Inducible Kaposi's Sarcoma-Associated Herpesvirus Transcripts in the K4 to K7 Region
... Through Northern blot analysis, the alternative K3 transcript was identified, and riboprobes confirmed its di- rectionality on the minus ...with Northern blotting and 5⬘ ...
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A promoter for the highly spliced EBNA family of RNAs of Epstein-Barr virus.
... Using Northern blotting, Si nuclease mapping, and primer extension analysis, we have identified the transcription start of some or all of these mRNAs in B95-8 cells in the Us region of t[r] ...
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Expression of pro opiomelanocortin gene and quantification of adrenocorticotropic hormone like immunoreactivity in human normal peripheral mononuclear cells and lymphoid and myeloid malignancies
... Using Northern blotting with a human genomic DNA probe for the pro-opiomelanocortin (POMC) gene, we have shown specific mRNA in normal human peripheral mononuclear cells (PBMC); the presence of specific ...
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Disruption of LDL receptor gene in transgenic SREBP 1a mice unmasks hyperlipidemia resulting from production of lipid rich VLDL
... Figure 1 compares the levels of various mRNAs, as determined by Northern blotting, in pooled samples from livers of wild-type mice, transgenic SREBP-1a mice TgBP1a, LDLR–/– mice, and TgB[r] ...
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Down-Regulation of Laminin-5 in Breast Carcinoma Cells
... (B) Northern blotting of ln-5 mRNAs in breast luminal epithelial and myoepithelial cells im- munomagnetically sorted from primary cultures of normal breast tissue.. Normal cultured 76N c[r] ...
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Molecular cloning and nucleotide sequence of complementary DNAs encoding human short chain acyl coenzyme A dehydrogenase and the study of the molecular basis of human short chain acyl coenzyme A dehydrogenase deficiency
... by Northern blotting using one of the normal SCAD cDNA as a probe was also normal, and no difference was observed on Southern blots in the restriction patterns of mutant genomic DNA using EcoRI, TaqI, ...
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EBV-miR-BART10-3p facilitates epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma by targeting BTRC
... According to bioinformatics analysis, we predicted that the BTRC gene might be regulated by multiple EBV encoded miRNAs, such as BART4, BART4*, BART6-3p, BART10-3p, BART18-5p, and BART19-5p [22]. To verify this ...
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Hormonal regulation of the humoral innate immune response in Drosophila melanogaster
... performed northern blotting on RNA extracted from S2* cells, using probes for the AMP genes Dpt, Cecropin and Attacin ...by northern blotting ...
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ppat.1007036.pdf
... using Northern blotting (Fig 2B and ...by Northern blotting (0–2 h for WT and 0–3 for EG, Fig 2 and S1 Fig), thus adding a phase to the kinetics of RNA synthesis monitored by luciferase ...by ...
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Prevalence and prognostic and predictive relevance of PRAME in breast cancer
... by Northern blotting and RT-PCR indicated PRAME to be expressed by most melanomas (~97%), neuroblastomas (93%), sarcomas (~80%), lung squamous cell carcinomas and small-cell lung cancers (~70%), Wilms’ ...
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Preferential mRNA expression of prostromelysin relative to procollagenase and in situ localization in human articular cartilage
... The mRNA levels of prostromelysin, procollagenase, and TIMP in chondrocytes freshly isolated from neonatal, normal adult, and osteoarthritic cartilage, were assessed by Northern blotting[r] ...
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Transfer of 15 lipoxygenase gene into rabbit iliac arteries results in the appearance of oxidation specific lipid protein adducts characteristic of oxidized low density lipoprotein
... 15-LO-transduced cells contained human 15-LO mRNA and immunoreactive protein, as analyzed by Northern blotting and Western blotting, respectively, whereas no 15-LO mRNA or protein was de[r] ...
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Hepatitis B virus RNA element that facilitates accumulation of surface gene transcripts in the cytoplasm.
... This decrease in transcript levels was not an artifact of primer extension analysis, since Northern blotting confirmed that insertion of the SV40 polyadenylation signal into the NcoI sit[r] ...
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Yeast Frameshift Suppressor Mutations in the Genes Coding for Transcription Factor Mbf1p and Ribosomal Protein S3: Evidence for Autoregulation of S3 Synthesis
... Southern blotting to carry the wild-type SUF14 allele to the RPS3 gene on chromosome 14, which encodes and not the SUF14::TRP1 disruption (data not ...by Northern blotting (data not ...Southern ...
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Phosphatidylserine synthase 2 and phosphatidylserine decarboxylase are essential for aminophospholipid synthesis in Trypanosoma brucei
... Down-regulation of TbPSD mRNA was analyzed by Northern blotting after 3 days of RNAi induction and showed efficient depletion of TbPSD mRNA in both procyclic and bloodstream form parasit[r] ...
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Equine infectious anemia virus trans-regulatory protein Rev controls viral mRNA stability, accumulation, and alternative splicing.
... The Northern blotting and PCR analyses of viral mRNAs expressed in cells transfected with cloned proviruses presented here corroborate previous data indicating that EIAV produces five ma[r] ...
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Differentiation of Larva Migrans Caused by Baylisascaris procyonis and Toxocara Species by Western Blotting
... (SDS–12% PAGE). The gels were stained with either Coomassie brilliant blue R-250 (Bio-Rad Laboratories, Richmond, CA) or silver stained (22). For West- ern blotting, the resolved proteins from the gel were ...
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Reexamination of Alcohol Dehydrogenase Structural Mutants in Drosophila Using Protein Blotting
... The methods used and the parameters estimated by each method are as follows: (1) native and urea- denaturing polyacrylamide gel isoelectric focusing (PAGIEF) for determi[r] ...
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