nuclear extract
Development of an in vitro pre-mRNA splicing assay using plant nuclear extract
... The nuclear extract preparation method presented here is a modification of protocols described previously [58–60]. For plant material preparation, seeds (50 mg) of Arabi- dopsis thaliana ecotype Columbia-0 ...
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Integrated Hepatitis B Virus DNA Preserves the Binding Sequence of Transcription Factor Yin and Yang 1 at the Virus-Cell Junction
... In this study, we detected a cellular protein which bound to the IS of HBV DNA at the virus-cell junction in the nuclear extract derived from HepG2 cells and was designated IS bind- ing protein 3 (ISBP3). ...
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SR protein splicing factors interact with the Rous sarcoma virus negative regulator of splicing element.
... from nuclear extract of cross-linked p36 by anti-SF2 antibodies and the lack of specific cross-linking of baculovirus-expressed SC35 to the NRS (data not shown) are strong evidence that p36 is ...
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Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome.
... To test the applicability of this method for measuring the binding of polyomavirus large T antigen to specific viral DNA fragments, we incubated increasing amounts of nuclear extract fro[r] ...
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Triplex formation inhibits HER 2/neu transcription in vitro
... transcription of this gene. We have shown that this sequence is a site for nuclear protein binding by incubation with a crude nuclear extract. We describe the formation of an interstrand triplex ...
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Nucleoprotein complexes of minute virus of mice have a distinct structure different from that of chromatin.
... When a nuclear extract containing both the MVMi nucleoprotein complexes and fragments of cellular chromatin was analyzed by sucrose gradient velocity centrifugation and agarose gel elect[r] ...
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The aspirin induced long non coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation
... the nuclear extract using the biotin- labeled OLA1P2 promoter. Nuclear proteins of CRC cells were incubated and pulled down by biotin-labeled OLA1P2 ...or nuclear receptor ...
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Potentiation of human immunodeficiency virus type 1 Tat by human cellular proteins.
... cytoplasmic extract (S-100) was as effective as preincuba- tion with the nuclear extract ...Both nuclear and cytoplasmic extracts from the thymocyte-derived Jurkat cell line were similarly ...
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A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription.
... Sequences within the D272 fragment which bind to factors within the HeLa cell nuclear extract were identified by using three different footprinting procedures.. To determine the correct [r] ...
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Polyoma virus minichromosomes: characterization of the products of in vitro DNA synthesis.
... The products of in vitro DNA synthesis in the nuclear extract were examined by two-dimensional neutral-alkali agarose gel electrophoresis after various lengths of incubation time in vitr[r] ...
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Regulation of miR-483-3p by the O-linked N-acetylglucosamine transferase links chemosensitivity to glucose metabolism in liver cancer cells.
... the nuclear lysis buffer added to the ...protein nuclear extract was sonicated using an IKA Labor technik U200 S Control apparatus (IKA-Works, Staufen im Breisgau, Germany, 30% amplitude, ...
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The cellular YY1 transcription factor binds a cis-acting, negatively regulating element in the Epstein-Barr virus BRLF1 promoter.
... cell nuclear extract and competed with cold oligonucleotides containing either the Sp1 binding motif, the NF-kB binding motif, sequences spanning the upstream BRLF1 promoter YY1 binding site (lane YY1-2), ...
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Purification of a mouse nuclear factor that binds to both the A and B cores of the polyomavirus enhancer.
... 4808 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have previously identified a protein factor, PEBP2 polyomavirus enhancer-binding protein, in the nuclear extract[r] ...
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Characterization of the Binding of a Potent Synthetic Androgen, Methyltrienolone, to Human Tissues
... The steroid specificity of binding of [3H]R 1881 to the nuclear extract of male sex accessory tissues is thus distinctly different from the binding to cytosol and bears the characteristi[r] ...
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Induction of anti-EBNA-1 protein by 12-O-tetradecanoylphorbol-13-acetate treatment of human lymphoblastoid cells.
... To determine the origin of the TPA-induced factors that inhibits the binding of the 68-kDa EBNA-1 to the EBV oriP, a nuclear extract from TPAactivated Raji cells was allowed to react wit[r] ...
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Transcriptional trans activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors.
... HeLa nuclear extracts passed through Gst-Tat affinity columns showed a small reduction of basal transcription from the HIV-1 and AdML ...depleted extract could be reconstituted with unfractionated ...
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Late replicative intermediates are accumulated during simian virus 40 DNA replication in vivo and in vitro.
... Alkaline sucrose gradient analysis of replicating molecules in the Hirt extract and in the hypotonic nuclear extract.. Parallel, infected cultures were labeled for 3 min with [3H]thymidi[r] ...
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Polyoma virus minichromosomes: a soluble in vitro replication system.
... Sedimentation analysis of the products of in vitro DNA synthesis by the nuclear extract showed that the majority of labeled DNA sedimented with the same distribution approximately 84S as[r] ...
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Inhibition of adenovirus DNA replication by vesicular stomatitis virus leader RNA.
... Specific replication in the presence of leader RNA was restored when the concentration of adenovirus-infected or uninfected nuclear extract was increased or by the addition of purified p[r] ...
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Proteomic analysis of mismatch repair-mediated alkylating agent-induced DNA damage response
... between nuclear extract and whole cell extract allowed us to identify an important phenomenon asso- ciated with MNNG treated TK6 cells, ...the nuclear proteome of MNNG-treated TK6 cells, but ...
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