nuclear extracts
In vitro transactivation of baculovirus early genes by nuclear extracts from Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells.
... In order to characterize the transcription of early promoters in vitro, the ability of nuclear extracts from uninfected Sf9 cells to transcribe a variety of other baculovirus genes was c[r] ...
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Differential transcription of baculovirus late and very late promoters: fractionation of nuclear extracts by phosphocellulose chromatography.
... Preparation of nuclear extracts. S. frugiperda (Sf9) cells were cultured and infected with the E2 strain of AcNPV as previously described (28). Nuclear extracts were prepared from uninfected ...
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Temperature-sensitive initiation and elongation of adenovirus DNA replication in vitro with nuclear extracts from H5ts36-, H5ts149-, and H5ts125-infected HeLa cells.
... VAN DER VLIET* Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands Adenovirus DNA replication was studied in vitro in nuclear extracts prepared [r] ...
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Deoxyinosine repair in nuclear extracts of human cells
... We first tested G-I repair in nuclear extracts from a HeLa cell line. In a preliminary trial, reaction conditions for both E. coli dI repair [9, 11] and human MMR [26] were tested. To our surprise, the G-I ...
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Proteomic Study of Hepatic Nuclear Extracts in an Adaptive Acetaminophen Tolerance Model
... crude nuclear lysates (15 μg per lane) were loaded onto 4–20% gels (Bio-Rad Laboratories, Hercules, CA, USA) prior to sodium dodecyl sulfate polyacrylamide gel ...
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A mouse mammary tumor virus promoter element near the transcription initiation site.
... A similar sequence was observed near the major transcription initiation site for the SV40 late promoter, and a protein in crude nuclear extracts was shown to footprint on SV40 sequences [r] ...
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Activation of STAT transcription factors by herpesvirus Saimiri Tip-484 requires p56lck.
... selected. Nuclear extracts were made and EMSAs were performed as described ...with nuclear extracts for 20 min at room temperature prior to addition of the radiolabeled probe and ...
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Regulation of avian leukosis virus long terminal repeat-enhanced transcription by C/EBP-Rel interactions.
... T-cell nuclear extracts ...clear extracts from B cells treated with protein synthesis inhib- itors while they are stable in T cells, suggesting that they are important for labile LTR-enhanced ...
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In Vivo Association of Adenovirus Large E1A Protein with the Human Mediator Complex in Adenovirus-Infected and -Transformed Cells
... When nuclear extract from uninfected HeLa cells is sub- jected to chromatography on Superose 6, Mediator subunits and Pol II are generally not detected to a similar extent in fractions corresponding to the region ...
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Regulation of bone formation and remodeling by G protein coupled receptor 48
... Our data demonstrate that deletion of Gpr48 in primary osteoprogenitor cells significantly reduced the binding of nuclear extracts to the Atf4 CRE site, as compared with nuclear extracts[r] ...
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La Autoantigen Specifically Recognizes a Predicted Stem-Loop in Hepatitis B Virus RNA
... liver nuclear extracts with an anti-La anti- ...clear extracts analyzed on the same ...the nuclear extracts by the anti-La antiserum ...
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Helix-loop-helix transcriptional activators bind to a sequence in glucocorticoid response elements of retrovirus enhancers.
... Complexes representing proteins binding with high nucleotide sequence specificity were detected for all nuclear extracts analyzed, but both the set of complexes obtained and the amounts [r] ...
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Possible Involvement of Nuclear Factor Kappa B in Rapid Disease Progression in ART Naive HIV 1 Infected Remunerated Blood Donors with History of Oral Iron Intake
... viz. nuclear factor kappa B (NF-κB), of which the main 65kDa transactivation do- main, ...in nuclear extracts of peripheral blood mononuclear cells (PBMCs) stimulated by Lipopo- lysaccharide (LPS) in ...
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Tissue specific regulation of CYP2B gene expression
... when extracts from the livers of untreated, picrotoxin- and PB-treated rats were incubated with the radiolabelled -2 to -178 probe ...Liver nuclear extracts from treated and untreated animals were ...
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A fourth Sp1 site in the human immunodeficiency virus type 1 long terminal repeat is essential for negative-sense transcription.
... using nuclear extracts from other cell lines indicated that complexes with mobility comparable to those of complexes 13a to 13c were present in CEM and U937 cells and that a fourth complex (13d) was present ...
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Recombinant influenza virus polymerase: requirement of both 5' and 3' viral ends for endonuclease activity.
... Nuclear extracts of cells that express influenza virus polymerase through recombinant vaccinia virus infection did not display specific endonuclease activity in vitro.. This polymerase p[r] ...
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In vitro replication of adeno-associated virus DNA.
... Crude nuclear extracts of baculovirusexpressed Rep78, Rep68, and Rep52 and a purified preparation mono-Q fraction of baculovirus Rep68 R68-Q were incubated in the standard replication as[r] ...
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Chicken ovalbumin upstream promoter transcription factor binds to a negative regulatory region in the human immunodeficiency virus type 1 long terminal repeat.
... A Comparison of COUP-TF binding in HeLa nuclear extracts to COUP and HIV-1 variant COUP oligonucleotide probes by gel mobility shift analysis.. HeLa nuclear extracts N.E.[r] ...
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A unique mitigator sequence determines the species specificity of the major late promoter in adenovirus type 12 DNA.
... Complex formation of the Adl2 MLP mitigator element and of related nucleotide sequences with proteins in nuclear extracts from hamster BHK21 or human HeLa cells.. Nucleotide sequences of[r] ...
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Distinct nuclear proteins competing for an overlapping sequence of cyclic adenosine monophosphate and negative regulatory elements regulate tissue specific mouse renin gene expression
... This binding was not inhibited by the preincubation of nuclear extracts with a 100fold molar excess of unlabeled oligonucleotides containing the mouse renin CRE or the hCG CRE, but was c[r] ...
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