nuclear-localization/import signal
Insertion of a Classical Nuclear Import Signal into the Matrix Domain of the Rous Sarcoma Virus Gag Protein Interferes with Virus Replication
... transient nuclear trafficking during virus assembly. Nuclear import is mediated by a nuclear targeting sequence within the MA ...of nuclear transport in replication, we investigated ...
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Probing the nuclear import signal and nuclear transport molecular determinants of PRV ICP22
... subcellular localization, localiza- tion signal and transport mechanism of ...cise nuclear localization signal (NLS) and its key amino acids (aa) of P-ICP22 was identified and mapped to ...
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A Nonconventional Nuclear Localization Signal within the UL84 Protein of Human Cytomegalovirus Mediates Nuclear Import via the Importin α/β Pathway
... Nuclear import assays. Nuclear import assays using permeabilized HeLa cells were exactly performed as described by Kann et al. (27). Briefly, digitonin- permeabilized HeLa cells grown on ...
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Nuclear Import and Export of Venezuelan Equine Encephalitis Virus Nonstructural Protein 2
... transport signal in the N-terminal third of nsP2, which led to a cytoplasmic and nuclear distribution of the fusion protein indistinguishable from the distribution of EGFP ...for localization of SFV ...
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Nuclear Import of Epstein-Barr Virus Nuclear Antigen 1 Mediated by NPI-1 (Importin α5) Is Up- and Down-Regulated by Phosphorylation of the Nuclear Localization Signal for Which Lys379 and Arg380 Are Essential
... (EBV) nuclear antigen 1 (EBNA-1) is essential for replication of episomal EBV DNAs and maintenance of ...on nuclear translocation of Ser phosphorylation of the EBNA-1 nuclear localization ...
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Identification of nuclear localization signal and nuclear export signal of VP1 from the chicken anemia virus and effects on VP2 shuttling in cells
... is import- ant for the virus during the viral life cycle and plays a vital role in the replication, assembly, and budding of ...CRM1-mediated nuclear export has been reported to be involved in several viral ...
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Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport.
... for nuclear trans- port of ...in nuclear import, different expression plasmids with deletions in the coding sequence for pp65 were con- structed and transfected into COS7 cells ...exclusively ...
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Nuclear Localization Signal of HIV-1 as a Novel Target for Therapeutic Intervention
... (1992) Active nuclear import of human immunodeficiency virus type 1 preintegra- tion complex. Stevenson M, Bukrinsky M, Haggerty S[r] ...
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Nuclear Import of Bovine Papillomavirus Type 1 E1 Protein Is Mediated by Multiple Alpha Importins and Is Negatively Regulated by Phosphorylation near a Nuclear Localization Signal
... its nuclear localization signal but not to importins ␣ 1 and ␣ ...the nuclear import of BPV1 E1 has been ...E1 nuclear import (28, ...
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Inhibition of HIV 1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal
... IN nuclear import is expected to result in the inhibition of virus replication, especially in cell-cycle arrested ...IN nuclear import inhibited the process of viral cDNA integration, reaching ...
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Dimerization and a Novel Tax Speckled Structure Localization Signal Are Required for Tax Nuclear Localization
... lular localization. The parental construct, which we have pre- viously described (16), provided an N-terminal S tag for pro- tein purification and a C-terminal GFP fusion to facilitate localization studies ...
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A nuclear targeting system in Plasmodium falciparum
... effective nuclear transport mechanism in ...GFP signal was found as early as in the ring stages and became more intense in the trophozoite and schizont stages (Figure ...GFP signal from every ...
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Characterization of the Nuclear Localization Signal of the Borna Disease Virus Polymerase
... Fine mapping of the L-NLS was performed by fusion of L to -galactosidase in order to ensure that fusion proteins were more than 60 kDa, the threshold for passive diffusion into the FIG. 3. B-galactosidase-L fusion ...
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DNA-binding activity associated with the v-myb oncogene product is not sufficient for transformation.
... Ni, Primary nuclear transport signal; N2, secondary signal which is required for complete nuclear localization in the presence of Ni; DNA-B, domain required for associated DNA-binding ac[r] ...
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Detection and Characterization of Influenza A Virus PA-PB2 Interaction through a Bimolecular Fluorescence Complementation Assay
... the nuclear localiza- tion of PA-GFP, concluding there was no obvious interaction between these two proteins ...some nuclear localization of PA protein, indicating that PB2 may interact with PA, ...
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Fabrication and characterization of nuclear localization signal-conjugated glycol chitosan micelles for improving the nuclear delivery of doxorubicin
... confocal laser scanning microscopy. As presented in Figure 8, most of the visible red fluorescence of DCHGC and DNCHGC micelles was in the nucleus, which is stained in blue with Hoechst 33342. Weak DOX fluorescence in ...
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Multifunctionality of a Picornavirus Polymerase Domain: Nuclear Localization Signal and Nucleotide Recognition
... a nuclear localization signal (NLS) (13), the arrows indicate the amino acids that interact with RNA (12), and boldface highlights the substituted ...
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Identification of a non-canonical nuclear localization signal (NLS) in BRCA1 that could mediate nuclear localization of splice variants lacking the classical NLS
... The primary sequence of the splice variants that were unequivocally cytoplasmic were compared with the variants that were at least part nuclear. The complete loss of exon 11 leads to total retention of the protein ...
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Functional Characterization of the Nuclear Localization Signal for a Suppressor of Posttranscriptional Gene Silencing
... arginine-rich nuclear localization sig- nal (NLS) that was essential for targeting C2 protein to nuclei and demonstrated that nuclear localization was likely required for the C2 protein to ...
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Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58)
... their localization or sequestration [56-60]. These find- ings regarding nuclear import and nucleolar localization signals that control subcellular localization and transcrip- tional ...
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