Nucleic acid amplification
Nucleic Acid Amplification Life Science Dashboard Series 3
... http://www.researchandmarkets.com/reports/1937244/ Nucleic Acid Amplification Life Science Dashboard Series 3 Description: Nucleic acid amplification is one of the most commonly ...
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Nucleic Acid Amplification Tests for Diagnosis of Neisseria gonorrhoeae Oropharyngeal Infections
... Jefferson County Department of Health, Birmingham, Alabama 3 ; Centers for Disease Control and Prevention, Atlanta, Georgia 4 ; and Birmingham Veterans Administration Medical Center, Birmingham, Alabama 5 Received 14 ...
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Towards Improved Accuracy of Bordetella pertussis Nucleic Acid Amplification Tests
... “Have no fear of perfection—you’ll never reach it.” —Salvador Dali T he laboratory diagnosis of pertussis has gone through funda- mental changes in the last 20 years. Culture and direct fluores- cent-antibody (DFA) ...
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Improved Sensitivity of Nucleic Acid Amplification for Rapid Diagnosis of Tuberculous Meningitis
... in-house nucleic acid amplification tests for the diagnosis of TBM, and the estimated values for sensitivity and specificity were 56 and 98%, respec- tively, for the 14 studies that used commercial ...
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Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA
... States, nucleic acid amplification assays have become more routinely used as methods of detecting arboviral RNAs for surveillance ...LAC nucleic acid amplification assays if used ...
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Original Article Screening blood donors by nucleic acid amplification technology in Turkey
... 1 Department of Medical Microbiology, Medical Virology Division, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey; 2 Department of Medical Microbiology, Meram Medical Faculty, Necmettin Erbakan ...
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Individual Donor Nucleic Acid Amplification Testing for Detection of West Nile Virus
... The nucleic acid amplification test (NAT) for WNV RNA in donated blood was implemented in June/July 2003 under an Investigational New Drug exemption issued by the FDA to two ...
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Nuclease Resistant Single Stranded DNA Controls for Nucleic Acid Amplification Assays
... alternative nucleic acid amplification technologies are commonly used for pathogen screening at blood drawing ...in nucleic acid amplification assays for pathogens with a ...
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The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
... primer-selective amplification of genotype variants in genomic DNA samples, ABI PCR mix led to lower background level for negative samples and smaller variation among different assays between positive and negative ...
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Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta analysis
... tuberculosis, Mycobacterium tuberculosis, nucleic acid amplification techniques, polymerase chain reaction, sen- sitivity and specificity, and accuracy. Experts in the field were contacted. ...
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Utility of cartridge based nucleic acid amplification test in the diagnosis of extra pulmonary tuberculosis
... Based Nucleic Acid Amplification Test) is an automated real time polymerase chain reaction assay designed for the rapid and simultaneous detection of Mycobacterium Tuberculosis (MTB) and Rifampicin ...
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Improving HIV 2 Detection by a Combination of Serological and Nucleic Acid Amplification Test Assays
... acid amplification. For HIV-1 RNA purification, the High Pure viral nucleic acid isolation kit was used with 500 l of plasma, according to the manufacturer’s instructions (Roche Diagnostics, ...
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Nucleic Acid Amplification Tests for Diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis Rectal Infections
... It is uncertain which methods for the diagnosis of rectal gonococcal and chlamydial infection are optimal. This study evaluated the performance of culture and nucleic acid amplification tests (NAATs) ...
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Comparison of Three Nucleic Acid Amplification Tests for Detection of Chlamydia trachomatis in Urine Specimens
... Johns Hopkins University, Baltimore, 1 and National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 2 Maryland Received 14 January 2004/Returned for modification 17 March ...
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Diagnostic value of nine nucleic acid amplification test systems for Mycobacterium tuberculosis complex
... The detection limits of these assay systems based on nucleic acid amplification for MTBC were less than 10 cells per reaction.All other laboratory NAAT results have a good sensitivity for SPss; how- ...
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Nucleic Acid Amplification Tests for Diagnosis of Bordetella Infections
... sequence which have been published. Proportion of positive samples by real-time Bordetella PCR formats compared to culture and block-based PCR formats a.. n Sample type Transport medium [r] ...
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Design and Validation of Transcription Mediated Amplification Nucleic Acid Amplification Tests for Mycoplasma genitalium
... In this study, we validated the performance characteristics of four TMA NAAT assays for the detection of ribosomal RNAs from M. genitalium. We found that these assays are sensitive and specific for the detection of ...
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Clinical evaluation of commercial nucleic acid amplification tests in patients with suspected sepsis
... DNA was extracted using 400 μL of sample volume and eluted in a final volume of 200 μL using a MagNA Pure Nucleic Acid Isolation Kit I on a MagNAPure Compact System (Roche Applied Science, Switzerland). The ...
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Diagnosis of mycobacterial infections by nucleic acid amplification: 18 month prospective study
... the amplification of the target ...the amplification reaction can be controlled for each individual ...leftover nucleic acids by the guanidium isothiocyanate ...
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Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis
... specific amplification of sequences found in patient samples or the contamination of laboratory ...nonspecific amplification in the Leptospira assay during the ana- lytical evaluation of the UFI assay or ...
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