Nucleic Acid based methods
Nucleic acid-based methods for on-site detection of plant pathogens : approaches and applications
... A nucleic acid-based test for a particular target comprises not only the mechanism for nucleic acid amplification, but also a means of determining whether amplification has ...detection ...
230
Nucleic Acid-Based Theranostics for Tackling Alzheimer's Disease
... Nucleic acids can also be introduced directly into the cerebrospinal fluid by intracerebroventricular infusion. The advantage of this over the use of targeting molecules is that the drugs are delivered at ...
15
Developing nucleic acid-based electrical detection systems
... for nucleic acid- based electrical detection has resulted in the construction of microchips and microarrays that contributed to micro- fluidic systems ...chip-based nucleic acid ...
8
Computational prediction of nucleic acid secondary structure: Methods, applications, and challenges
... is based on the hierarchical folding hypothesis, which is succinctly stated by Tinoco and Bustamante as follows: ‘‘An RNA molecule [has] a hierarchical structure in which the primary sequence determines the ...
8
iTriplet, a rule-based nucleic acid sequence motif finder
... Both enumerative and statistical approaches have proven to be valuable in analyzing real biological examples and both approaches are complementary to each other. In most situations when little prior knowledge is known ...
14
Understanding Nucleic Acid based Materials through Molecular Modeling.
... 16 dispersion and repulsion forces using Lennard-Jones potential. The explicit solvent method will be used for most of the systems because it produces the most accurate modeling of solvation effects and can provide ...
213
Nano-vectors for the ocular delivery of nucleic acid-based therapeutics
... Delivery Nucleic acid-based therapeutics have gained a lot of interest for the treatment of diverse ophthalmic ...of nucleic acids is a major challenge since therapeutic targets for ...
14
Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications
... 1. A single step isothermal amplification reaction at 41 0 C. 2. Especially suited for RNA analytes because of the integration of RT into the amplification process. 3. The single-stranded RNA product is an ideal target ...
16
Nucleic acid based molecular biological methods for quantitation and characterization of human cytomegalovirus in the clinical specimens from immunocompromised patients.
... PCR based RFLP since the former method allowed easier detection of mixed infections with multiple gB genotypes in clinical ...PCR based RFLP and required 5 hours for completion of the test as opposed to 10 ...
344
Nucleic and Amino Acid Sequences Support Structure-Based Viral Classification
... Our investigations suggest that public databases do not contain intermediate sequences. One can only speculate as to whether this is due to a fragmented occupa- tion of sequence space (53). Such fragmentation could be ...
13
Nucleic and amino acid sequences support structure-based viral classification
... Our investigations suggest that public databases do not contain intermediate sequences. One can only speculate as to whether this is due to a fragmented occupa- tion of sequence space (53). Such fragmentation could be ...
14
Diagnosis of paediatric tuberculosis by cartridge based nucleic acid amplification test and its effectiveness as compared to the other conventional diagnostic methods
... cartridge- based format removes the need for manual DNA extraction processing, and the closed system dramatically reduces any potential for cross-contamination of samples with PCR ...
7
Comparison of Deoxyribose Nucleic Acid Purification Methods of Mangosteen (Garcinia mangostana L.) and Its Relatives
... deoxyribose nucleic acid is difficult to ...deoxyribose nucleic acid purification method of mangosteen leaves and its ...isolated based on cetyl trimethyl ammonium bromide (CTAB) buffer ...
8
Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... XNA molecular beacon as a new sensor opened up new opportunities to monitor DNA/RNA in vitro and in vivo. Despite oligonucleotide-based fluores- cent probes have been greatly improved, by far, there are only a few ...
14
On nucleic acid photochemistry
... General features. Ultraviolet action spectra for the formation of thymine dimer in E. coli DNA are shown in figs. Qual- itatively, the spectra may be interpreted to mea[r] ...
73
Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes (US)
... activated only when the two strands bind to the target analyte reconstituting the catalytic core. The activated enzyme then cleaves the bound substrate generating a signal localized [r] ...
26
Targeting Nucleic Acid Junctions Using Triptycene-Based Molecules
... 47 have demonstrated that the introduction of geometric and macrocyclic constraints in nucleic acid binding small molecules can be used as a strategy to eliminate common binding modes such as intercalation ...
218
Comparison of Automated and Manual Nucleic Acid Extraction Methods for Detection of Enterovirus RNA
... extract nucleic acid for use in amplification assays is the potential for cross-contamination of negative specimens as a consequence of aerosolization, faulty robotics, or robotic ...both methods ...
5
Nucleic acid delivery to mesenchymal stem cells: a review of nonviral methods and applications
... condense nucleic acids and mediate their in- ternalization, trafficking, and expression within target cells ...of methods for efficient nonviral gene delivery to ...delivery methods are safer than ...
16