nucleic acid probe assay
Detection of respiratory syncytial virus in nasopharyngeal secretions by DNA RNA hybridization
... Four of these samples were negative for RSV by nucleic acid hybridization and one, from which adenovirus was isolated, was weakly positive; a control assay using the pBR322 probe was not[r] ...
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Multicenter Evaluation of the Verigene Clostridium difficile Nucleic Acid Assay
... For C. difficile testing on the Verigene system, 100 l of the prepared stool sample is loaded into the sample well of the extraction tray. The test specimen is prepared from a soft or liquid stool sample by swabbing ...
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Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi probe real time PCR method into sputa samples
... Previously, we introduced a multiprobe real-time PCR assay targeting hsp65 (HMPRT-PCR) to detect and iden- tify pathogenic mycobacteria directly from sputum speci- mens. In this study, we compared the 2 methods ...
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Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections
... the assay was defined as the ability to exclu- sively detect the target of ...of nucleic acid contamination than the species-spe- cific assays, which can lead to false-positive ...a nucleic ...
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Evaluation of Two Line Probe Assays for Rapid Detection of Mycobacterium tuberculosis, Tuberculosis (TB) Drug Resistance, and Non TB Mycobacteria in HIV Infected Individuals with Suspected TB
... the assay probe for ...line probe assay (Direct LPA) (also Hain Lifesciences GmbH) is an- other line probe assay that uses nucleic acid sequence-based am- ...
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Comparison of the APTIMA CT and GC Assays with the APTIMA Combo 2 Assay, the Abbott LCx Assay, and Direct Fluorescent Antibody and Culture Assays for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
... Administration-cleared nucleic acid amplifi- cation test (NAAT) for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae from urine and urogenital swab ...different nucleic acid ...
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PCR based assay for Mycoplasma hyopneumoniae
... The kit of claim 2 further comprising a probe compris ing the nucleotide sequence 5‘-GGTAGCCCITCCI'I'I‘GAGGT-3' SEQ ID NO: 3 that hybridizes to an ampli?ed target nucleic acid sequence r[r] ...
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Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay
... each dATP, dCTP, dGTP, and dTTP, 2 mM each ATP, CTP, and UTP, 1.5 mM GTP, 0.5 mM ITP, 0.1 g of bovine serum albumin (BSA)/l, 1.5 M sorbitol, 0.08 U of RNase H, 32 U of T7 RNA polymerase, 6.4 U of avian myeloblastosis ...
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Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis
... nosorbent assay of nucleic acid sequence-based amplification (NASBA-ELISA) was developed to fulfil the need for the efficient diagnosis of these ...DNA probe immobilized on streptavidin-coated ...
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<p>Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections</p>
... The assay involves one oligo- nucleotide (single-stranded) probe for extending sequences and another that serves as the ...opposite probe, thereby increasing the length of the extension probe ...
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Use of a Locked Nucleic Acid Oligomer in the Clamped Probe Assay for Detection of a Minority Pfcrt K76T Mutant Population of Plasmodium falciparum
... In contrast to a published assay (3) using full-PNA oli- gomers, we used mixed sequences containing both DNA and LNA monomers, which is not applicable for PNA. This manner of action ensures a fine-tuning of the ...
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Multicenter Evaluation of the Portrait Staph ID/R Blood Culture Panel for Rapid Identification of Staphylococci and Detection of the mecA Gene
... PCR assay (Great Basin Scientific, Salt Lake City, UT) for the rapid and simultaneous identification (ID) of Staphylococcus aureus, Staphylococcus lugdunensis, and Staphylococcus species to the genus level and the ...
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New Real Time PCR Assay Using Allelic Discrimination for Detection and Differentiation of Equine Herpesvirus 1 Strains with A2254 and G2254 Polymorphisms
... DNA extraction from archived and clinical materials was performed using four different methods. All purified archived viruses from the United States and the United Kingdom (1941 to 2006) were treated with proteinase K ...
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Development of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticles
... Abstract: Self-amplifying RNA or RNA replicon is a form of nucleic acid-based vaccine derived from either positive-strand or negative-strand RNA viruses. The gene sequences encoding structural proteins in ...
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Evaluation of the Analytical Performance of the Xpert MTB/RIF Assay
... the assay to detect the RIF-resistant fraction of a mixed ...the assay could detect the presence of resistance with 95% certainty when the mixture contained at least ...the assay could not detect the ...
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Evaluation of real time PCR for diagnosis of Bordetella pertussis infection
... casamino acid (Difco, Becton Dickison, Sparks, MD) diluted in ...casamino acid volume was split for bacteri- ology (DFA/culture) and molecular testing ...swab/casamino acid was used each for culture ...
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Comparison of Xpert MTB/RIF with ProbeTec ET DTB and COBAS TaqMan MTB for direct detection of M tuberculosis complex in respiratory specimens
... DTB assay requires multiple work-stations for specimen processing, priming, pre-amplification prior to automated SDA in the ProbeTec ET instrument adding up to a hands-on time of 50 min for 30 specimens in our ...
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DNA and PNA Synthesis Instruments and Consumables
... telomere probe is available labeled at the (5’) N-terminal with OO linker and either Cy3 or ...The probe is supplied in solution and has been optimized for in situ hybridization ...sufficient probe ...
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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... cleic acid greatly helped us study biological evolution, gene analysis, disease diagnosis, as well as drug dis- ...clear acid. Among these analogs, peptide nucleic acid (PNA) and locked ...
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Multicenter Evaluation of the Quidel Lyra Direct C difficile Nucleic Acid Amplification Assay
... Direct cell culture cytotoxicity neutralization assay. An aliquot of each stool specimen was processed within 48 h of collection for direct C. difficile CCNA. A 1:5 dilution was prepared by adding 0.5 ml of the ...
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