nucleocytoplasmic shuttling
Nucleocytoplasmic Shuttling of Human Cytomegalovirus UL84 Is Essential for Virus Growth
... of shuttling in the con- text of the virus genome, an HCMV bacterial artificial chro- mosome plasmid (BACmid) expressing the nonshuttling UL84 mutant (NS84 BAC) was generated and transfected into human ...a ...
11
Nucleocytoplasmic Shuttling of Bovine Herpesvirus 1 UL47 Protein in Infected Cells
... Previous studies with transfected cells have shown that the herpes simplex virus type 1 (HSV-1) and bovine herpesvirus 1 (BHV-1) UL47 proteins shuttle between the nucleus and the cytoplasm. HSV-1 UL47 has also been shown ...
5
Borna Disease Virus Nucleoprotein Requires both Nuclear Localization and Export Activities for Viral Nucleocytoplasmic Shuttling
... a nucleocytoplasmic shuttling ...demonstrate nucleocytoplasmic shuttling of the BDV N protein, we used an interspecies heterokaryon assay (2, 26, 50) in which human OL cells transiently ...
9
Nucleocytoplasmic Shuttling of Bovine Papillomavirus E1 Helicase Downregulates Viral DNA Replication in S Phase
... The papillomavirus E1 protein is essential for the initiation of viral replication. We previously showed that the bovine papillomavirus E1 protein is unstable and becomes resistant to ubiquitin-mediated degradation when ...
11
Nucleocytoplasmic shuttling mediates the dynamic maintenance of nuclear Dorsal levels during Drosophila embryogenesis
... While nucleocytoplasmic shuttling has been described for a number of transcription factors, our results extend the process to the formation of a developmental gradient during ...Dynamic shuttling ...
9
Apoptin Nucleocytoplasmic Shuttling Is Required for Cell Type-Specific Localization, Apoptosis, and Recruitment of the Anaphase-Promoting Complex/Cyclosome to PML Bodies
... The NLS and nucleocytoplasmic shuttling are required for APC1 interaction and Apoptin-induced cell death. The exper- iments described above address the contribution of the NES and NLS to protein ...
11
RNA Binding by the Herpes Simplex Virus Type 1 Nucleocytoplasmic Shuttling Protein UL47 Is Mediated by an N-Terminal Arginine-Rich Domain That Also Functions as Its Nuclear Localization Signal
... exhibit nucleocytoplasmic shuttling and to function in viral RNA export pathways ...UL47 nucleocytoplasmic shuttling has not yet been defined, it may well be linked to its RNA binding ...
14
The Second Open Reading Frame of the Avian Reovirus S1 Gene Encodes a Transcription-Dependent and CRM1-Independent Nucleocytoplasmic Shuttling Protein
... The p17 protein contains a CRM1-independent shuttling sequence. By performing interspecies heterokaryon assays and by using specific inhibitors of transcription, we have demon- strated that p17 is a ...
10
Nucleocytoplasmic Shuttling by Human Immunodeficiency Virus Type 1 Vpr
... Human immunodeficiency virus type 1 (HIV-1) is capable of infecting nondividing cells such as macrophages because the viral preintegration complex is able to actively traverse the limiting nuclear pore due to the ...
11
Nucleocytoplasmic Shuttling of the Rpb4p and Rpb7p Subunits of Saccharomyces cerevisiae RNA Polymerase II by Two Pathways
... of shuttling during stress, we shifted nup49-313 cells producing either GFP-Rpb4p or Rpb7p-RFP to 37°C to inactivate pro- tein import (see the results for nonpermissive temperature in ...
12
Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants.
... The difference in subcellular distribution between Rev wt and the three members of Rev TD is thus restricted to the export part of Rev nucleocytoplasmic shuttling. The woolly nucleoplasmic patches and ...
9
Nuclear Localization of the Duck Hepatitis B Virus Capsid Protein: Detection and Functional Implications of Distinct Subnuclear Bodies in a Compartment Associated with RNA Synthesis and Maturation
... a nucleocytoplasmic shuttling RNA binding protein (21), may also participate in the nuclear export of the unspliced pregenomic DHBV RNA, as exemplified by the role of structural virus proteins in the ...
8
Genome Segregation and Packaging Machinery in Acanthamoeba polyphaga Mimivirus Is Reminiscent of Bacterial Apparatus
... G enome delivery and packaging are fundamental processes in the life cycle of a virus. A large genome size (1) coupled with complex replication and assembly (2, 3) processes makes genome packaging in Acanthamoeba ...
7
p53 promotes the expression of gluconeogenesis-related genes and enhances hepatic glucose production
... the shuttling of glucose-6-phosphate (G6P) from the cytosol to the endoplasmic reticulum (where G6P is dephosphory- lated by G6Pase), which is essential for completion of glu- coneogenesis, was predicted to carry ...
6
Nuclear-Cytoplasmic Shuttling Is Not Required for the Epstein-Barr Virus EBNA-LP Transcriptional Coactivation Function
... active shuttling between these cellular compartments. Whether active shuttling between the nucleus and cytoplasm is required for coactivation remains to be ...nuclear-cytoplasmic shuttling activity ...
8
<p>Role of Janus kinase 1 and signal transducer and activator of transcription 3 in vitiligo</p>
... Notes: ( A ) Nucleocytoplasmic JAK1 expression in epidermis in control skin; ( B ) cytoplasmic expression of JAK1 in eccrine and sweat-gland ducts in control skin; ( C ) Epidermal cytopl[r] ...
12
<p>Does the Autophagy Related Gene 7 (ATG7) Have a Role in Non-Melanoma Skin Cancer?</p>
... Moreover, Huang and Liu 33 examined the essential role for nuclear LC3 in starvation-induced autophagy. LC3 is distributed in an acetylated shape in the nucleus as well as in cytoplasm during nutrient rich circumstances. ...
10
RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5
... Although we identified a nucleocytoplasmic type-I Hsp40 (Mas5) as a silencing factor that is essential for RNAi-dependent heterochromatin assembly, we demon- strated that the double-nu[r] ...
15
Herpesvirus mRNAs Are Sorted for Export via Crm1-Dependent and -Independent Pathways
... Functional analysis of ICP27. Previous results demonstrated that three independent intragenic suppressors of a conditional lethal ts mutation in ICP27 restore shuttling and virus growth (75). These results ...
12
A Single Amino Acid Substitution in the ICP27 Protein of Herpes Simplex Virus Type 1 Is Responsible for Its Resistance to Leptomycin B
... ICP27, shuttling between the nuclear compart- ments and the cytoplasm (19, 28, 32, 35), acts at multiple steps in the life cycle of the virus (reviewed in reference ...
5