nucleoprotein complex
Polyoma Viral DNA Replicated as a Nucleoprotein Complex in Close Association with the Host Cell Chromatin
... 567-576 Copyright 0 1974 American Society for Microbiology Polyoma Viral DNA Replicated as a Nucleoprotein Complex in Close Association with the Host Cell Chromatin THOMAS SEEBECK' AND R[r] ...
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Targeting APOBEC3A to the viral nucleoprotein complex confers antiviral activity
... In this study we wanted to investigate why A3A lacks anti- viral activity against HIV-1. We observed that A3A was packaged into HIV-1 virions but did not associate with the viral nucleoprotein complex (NPC) ...
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SSINCC: Simple separation of interacting nucleoprotein complex components
... The proposed simple SSINCC capture assay employ- ing biotin-streptavidin-DNA coated magnetic beads and radiolabeled bait can be applied to study protein-DNA interaction at large. The technique need not be limited to ...
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RNA protects a nucleoprotein complex against radiation damage
... dynamics between RNA-bound and nonbound TRAP (p > 0.9). An increase in the dose at which functionally important residues remain intact has biological ramifications for under- standing the mechanisms at which ionizing ...
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Human Immunodeficiency Virus Type 1 Vif Protein Is Packaged into the Nucleoprotein Complex through an Interaction with Viral Genomic RNA
... their nucleoprotein or reverse transcription complexes (18, 46, 55) and are impaired in the reverse transcription of their genomes (13, 43, 55, 61, ...viral nucleoprotein complexes as well as the ...
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Efficient Transcription of a Compact Nucleoprotein Complex Isolated from Purified Simian Virus 40 Virions
... Due to the relative simplicity of simian virus 40 SV40 nucleoprotein complexes and the ability to isolate SV40 DNA, DNA-histone minichromosome, and DNA-histone viral protein core complex[r] ...
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Comparison of nuclease digestion of polyoma virus nucleoprotein complex and mouse chromatin.
... Lengths of DNA fragments obtained from digestion ofpolyoma and mouse chromatin with micrococcal nuclease" DNA fragment Length of DNA fragment base pairs Polyoma virions Mouse nuclei 0.3b[r] ...
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Use of streptavidin bound to biotinylated DNA structures as model substrates for analysis of nucleoprotein complex disruption by helicases
... of nucleoprotein complexes present accidental barriers to helicase movement along their substrates ...by nucleoprotein complexes to the completion of genome ...
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Cell-free translation of RNA synthesized in vitro by a transcribing nucleoprotein complex prepared from purified vesicular stomatitis virus.
... Track 1, Virion polypeptides of Chandipura virus; track 2, in vitro synthesized polypeptides in the presence of transcription mixture containing TNP Chandipura; track 3, virion polypepti[r] ...
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Genetic Analysis of the Bacteriophage λ attL Nucleoprotein Complex
... In each case, the mutant protein was altered in its ability to form attL complexes while retaining its ability to bind to the A arm-type sites.. We propose that, i[r] ...
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Biosynthetic Properties of a Polyoma Nucleoprotein Complex: Evidence for Replication Sites
... In the pulse-chase experiments, the whether the py DNA in the 55S complex can radioactive medium was removed by aspiration, the 32 Downloaded from http://jvi.asm.org/ on November 10, 201[r] ...
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A quantum chemical model of the inhibition mechanismof viral DNA HIV 1 replication by Iodine complexcompounds
... The results of the study are summarized in three sec- tions. The first section deals with the selected computa- tional method tested on model structures. The structure and nature of the molecular iodine interaction with ...
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Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import.
... MA is the N-terminally myristoylated cleavage product of the viral Gag precursor, whose membrane association is crucial for particle assembly and release (42). MA has intrinsic karyo- philic properties conferred by a ...
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Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.
... Viral DNA can be purified from these extracts by gel filtration chromatography and sucrose gradient sedimentation as a part of a nucleoprotein complex containing integrase as the only vi[r] ...
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The adenovirus EII early promoter has multiple EIA-sensitive elements, two of which function cooperatively in basal and virus-induced transcription.
... By evaluating the contributions of each transcriptional control element in both basal and EIA-mediated activation when these elements are organized in the milieu of nucleoprotein complex[r] ...
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Distribution of DNase I-sensitive sites in simian virus 40 nucleoprotein complexes from disrupted virus particles.
... Alternatively, the nucleoprotein Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Nucleoprotein complexes core particles released from simian virus 40 SV40 virions were [r] ...
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Crystal structure of Schmallenberg orthobunyavirus nucleoprotein RNA complex reveals a novel RNA sequestration mechanism
... in complex with a 42-nt-long RNA to 2.16 Å resolution. The complex comprises a tetramer of N that encapsidates the RNA as a cross-shape inside the protein ring structure, with each protomer bound to 11 ...
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The Antiviral Mechanism of an Influenza A Virus Nucleoprotein Specific Single Domain Antibody Fragment
... FIG 3 ␣ NP-VHH1 binds to the body domain of NP. (A) NP alone (blue) or preincubated with an excess of ␣ NP-VHH1 (red) was subjected to size exclusion chromatography with a Superdex 200 column. Absorbance at 280 nm of the ...
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Identification of influenza A nucleoprotein body domain residues essential for viral RNA expression expose antiviral target
... Background: Influenza A virus is controlled with yearly vaccination while emerging global pandemics are kept at bay with antiviral medications. Unfortunately, influenza A viruses have emerged resistance to approved ...
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CNOT4 Mediated Ubiquitination of Influenza A Virus Nucleoprotein Promotes Viral RNA Replication
... To understand the possible mechanism of enhancements of RdRp activity by ubiquitination of these five lysine residues, we further analyzed the locations of these lysines on the NP molecule (Fig. 6B). The crystal structure ...
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