Nucleus pulposus (NP) cells
Original Article Resveratrol inhibits TNF-α-induced matrix degradation via the p38/MAPK and Akt pathways in human nucleus pulposus cells
... disc cells [27], as well as to decrease pro-inflammatory cytokines and catabolic enzymes expression in human IVD cells ...human nucleus pulposus (NP) ...
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Differential response of encapsulated nucleus pulposus and bone marrow stem cells in isolation and coculture in alginate and chitosan hydrogels
... autologous nucleus pulposus (NP) cells delays degenerative changes in the disc (10, ...of NP cells that can be harvested and expanded in vitro are insufficient to meet ...
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Regulatory Role of Hypoxia Inducible Factor in the Biological Behavior of Nucleus Pulposus Cells
... of nucleus pulposus (NP) cells may be key contributors to progressive IVD ...NP cells. We describe current data on the ex- pression of HIF in NP cells and further ...
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Extracellular matrix production by nucleus pulposus and bone marrow stem cells in response to altered oxygen and glucose microenvironments
... Figure 2 A Total sGAG normalized to wet weight and to DNA content at day 21 for nucleus pulposus NP and bone marrow BM stem cells maintained under different glucose conditions 1mM, 5mM, [r] ...
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Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells
... progenitor cells from hiPSCs to test their preferred utility in generating NP ...transfected cells were selected by culture with 10 mg/ml of Geneticin (G418; Invitrogen) for 4 ...in cells was ...
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Original Article Differentiation of immortalized human precartilaginous stem cells into nucleus pulposus-like cells
... primitive cells in vitro, with a long-term and stable role in gene therapy with- out adverse effects ...stem cells (IPSCs) as described in a previous study ...stem cells into NP-like ...
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Original Article Nucleus pulposus cells derived IGF-1 and MCP-1 enhance osteoclastogenesis and vertebrae disruption in lumbar disc herniation
... Since a positive relationship was found between the bone resorption in vertebrae and herniated lumbar disc, we hypothesized that osteoclast (OC) differentiation or activity may be enhanced during the lumbar disc ...
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Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase related genes in nucleus pulposus cells in rabbits
... called nucleus pulposus, annulus fibrosus, and cartilage end plates ...The nucleus pulposus of normal disc includes sparse chondrocytes surrounded by extracellular matrix which mainly consist ...
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A complex interaction between Wnt signaling and TNF α in nucleus pulposus cells
... nucleus pulposus. During development, the highly hydrated nu- cleus pulposus is populated by clusters of large vacuolated notochordal cells of distinct molecular ...notochordal cells ...
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Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
... endothelial cells, the angiopoietin groups of ligands, upon binding to their receptor Tie2, are known to regulate angiogenesis ...primary nucleus pulposus cells (NPC) from bovine coccygeal ...
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Is Osteogenic Differentiation of Human Nucleus Pulposus Cells a Possibility for Biological Spinal Fusion?
... human NP cells maintained for 21 days with osteogenic medium (osteo) (B1-4) and its components, dexamethasone (DEX) (C1-4), β-glycerophosphate (B--G) (D1-4), L-ascorbic acid-2-phosphate (AA) (E1-4) and in ...
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Acidification changes affect the inflammasome in human nucleus pulposus cells
... CA) cells were obtained from the spinal column (isolated from the nu- cleus pulposus of human intervertebral discs) of a male donor that did not present spine degenerative ...disease. Cells were ...
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Original Article RASSF7 expression and its regulatory roles on apoptosis in human intervertebral disc degeneration
... degenerative nucleus pulposus (NP) cells and non-degenerative NP cells and the link between RASSF7-JNK with NP cells ...harvested NP tissues from 20 IDD ...
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CD24 identifies nucleus pulposus progenitors/notochordal cells for disc regeneration
... CD24-positive NP cells to disc degeneration repair, first, the pheno- types of CD24-positive NP cells were ...CD24-negative NP cells were iso- lated from rat NP tissues by ...
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Differential expression level of cytokeratin 8 in cells of the bovine nucleus pulposus complicates the search for specific intervertebral disc cell markers
... bovine NP cells and six independent isolations of ACs were carried ...Bovine NP cells, AF cells, and ACs were isolated by an overnight enzymatic digestion using ...notochordal ...
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Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-α-induced senescence
... notochordal cells (NCs) have been found in the embryonic notochord structure of chordates [21]; human NCs disappear at approximately age 10, whereas the degeneration of the IVD is initiated shortly after this ...
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The cytokine and chemokine expression profile of nucleus pulposus cells: implications for degeneration and regeneration of the intervertebral disc
... In degeneration, alterations in native NP cell behaviour occur in respect of proliferation [5], differentiation [6-8], dysregulated metabolism [3,9] and cell death [10,11]. Cy- tokines are implicated as stimulus ...
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Identification and characterization of human nucleus pulposus cell specific serotypes of adeno associated virus for gene therapeutic approaches of intervertebral disc disorders
... processes. Nucleus pulposus (NP) cells play a pivotal role in maintenance of IVD by organizing the expression of anabolic, catabolic, anti-catabolic and inflammatory ...Human NP ...
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IL 1ra delivered from poly(lactic co glycolic acid) microspheres attenuates IL 1β mediated degradation of nucleus pulposus in vitro
... [16]. NP tissue was isolated via sharp dissection from the intervertebral discs of four bovine caudal spines purchased from a local slaughterhouse according to insti- tutional ...(PSF). Cells were isolated ...
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Biologic Response of Degenerative Living Human Nucleus Pulposus Cells to Treatment with Cytokines
... IVD cells for alkaline phosphatase increased after treatment with rhBMP-2 and TGF-β in group ...IVD cells showed strong immunoreactivity and well- demarcated ...
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