O-antigen
2F3 Monoclonal Antibody Recognizes the O26 O-Antigen Moiety of the Lipopolysaccharide of Enterohemorrhagic Escherichia coli Strain 4276
... entire O-antigen gene cluster from an EHEC strain into a rough-type LPS strain which is 2F3 ELISA negative, a 2F3 ELISA-positive clone with a smooth- type LPS was ...O26 O antigen recognized ...
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Identification of The Role of O-antigen of Nonmucoid Klebsiella pneumoniae During Bacteriophage NJS1 Infection
... encoding O-antigen polymerase showed no significant difference in phage adsorption but increased phage infectivity, suggesting the long chain length of O-antigen may also be a barrier for ...
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Loss of Very Long O Antigen Chains Optimizes Capsule Mediated Immune Evasion by Salmonella enterica Serovar Typhi
... very-long O-antigen ...very-long O-antigen ...very-long O-antigen chains by pseudogene formation was an adaptation to maxi- mize the anti-inflammatory properties of the Vi ...
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PEGylation as a novel tool to investigate the topology of Escherichia coli WecA, a membrane enzyme involved in lipopolysaccharide O antigen initiation
... of O-antigen starts by the formation of a phosphoanhydride bond linking a sugar phosphate with a membrane isoprenoid lipid ...in O-antigen ...
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Evidence obtained with monoclonal antibodies that O antigen is the major antigen responsible for the cross-reactivities between serotypes 4 and 7 of Actinobacillus (Haemophilus) pleuropneumoniae.
... of O antigen shared by serotypes 4 and 7 ...within O antigen of serotype 4. Specific LPS antigenic O-chain component is always associated with a specific capsular polysaccharide in ...
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Serological Identification and Prevalence of a Novel O Antigen Epitope Linked to 3 and 4 O Acetylated Rhamnose III of Lipopolysaccharide in Shigella flexneri
... new O-acyltransferase gene named oacB has been shown to mediate the 3/4-O-acetylation in serotypes 1a, 1b, 2a, 5a, and Y but not in ...group O factor ...of O-antigenic determinants related to ...
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Regulated Delayed Shigella flexneri 2a O-antigen Synthesis in Live Recombinant Salmonella enterica Serovar Typhimurium Induces Comparable Levels of Protective Immune Responses with Constitutive Antigen Synthesis System
... [22], O-antigen structures are believed to be the primary targets of the immune ...Shigella O-antigen play a key role in affording protection ...Shigella O-antigen in rough ...
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Ectopic Expression of O Antigen in Bordetella pertussis by a Novel Genomic Integration System
... in O antigen biosynthesis of Bordetella bron- chiseptica into the chromosome of ...lacks O antigen, and using this system we analyzed phenotypic alterations in the resultant mutant strain of ...
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Sequencing of Escherichia coli O111 O Antigen Gene Cluster and Identification of O111 Specific Genes
... genes. O-antigen gene clus- ters generally contain about 8 to 20 genes that fall into three general classes: (i) genes for the synthesis of nucleotide sugar precursors such as dTDP-rhamnose or ...
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Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase
... of O units for O-antigen synthesis requires a specific regulatory process and that this involves a sufficiency of O units being detected by the periplasm-exposed part of the WaaL ...abolish ...
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A BTP1 prophage gene present in invasive non-typhoidal Salmonella determines composition and length of the O-antigen of the lipopolysaccharide
... the O-antigen of the lipopolysac- charide (LPS) and flagellar H antigen to differentiate between ...O4 O-antigen (Grimont and Weill, 2007), which is characterized specifically by an ...
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Laboratory adapted Escherichia coli K 12 becomes a pathogen of Caenorhabditis elegans upon restoration of O antigen biosynthesis
... Laboratory adapted Escherichia coli K-12 becomes a pathogen of Caenorhabditis elegans upon restoration of O antigen biosynthesis... Manuscript ID: Manuscript Type: Date Submitted by the [r] ...
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Proton Dependent Gating and Proton Uptake by Wzx Support O Antigen Subunit Antiport Across the Bacterial Inner Membrane
... distal O-antigen (O-Ag) polymer. Heteropolymeric O-specific (B- band) O-Ag is the predominant surface antigen, the presence of which affects a range of virulence phenotypes, ...
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Identification of Escherichia coli O114 O Antigen Gene Cluster and Development of an O114 Serogroup Specific PCR Assay
... Genes for O-unit processing. Wzx and Wzy are typical inner membrane proteins with more than nine transmembrane seg- ments. Wzy also typically contains a large periplasmic loop of more than 30 amino acids. ORF7 had ...
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Development of a Multiplex PCR Assay Targeting O Antigen Modification Genes for Molecular Serotyping of Shigella flexneri
... on O-antigen ...the O-antigen synthesis gene wzx and the O-antigen modification genes gtrI, gtrIC, gtrII, oac, gtrIV, gtrV, and gtrX for molecular serotyping of ...
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Identification of an Escherichia coli operon required for formation of the O antigen capsule
... saccharides (LPS and capsular polysaccharide) were extracted, and the capsular polysaccharide was separated from the LPS. Production of LPS O antigen was analyzed by Western blotting (A), and the amount of ...
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O Antigen Diversity among Acinetobacter baumanniiStrains from the Czech Republic and Northwestern Europe, as Determined by Lipopolysaccharide Specific Monoclonal Antibodies
... the O-antigenic rela- tionship among these Czech strains, in comparison to a num- ber of clone I and II strains, by using O-antigen-specific ...Acinetobacter O-serotypes (i.e., the ...
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Rapid and Accurate Determination of Lipopolysaccharide O Antigen Types in Klebsiella pneumoniae with a Novel PCR Based O Genotyping Method
... (LPS) O-antigen serotypes. The O-antigen type has important clini- cal and epidemiological ...pneumoniae O serotyping is cumbersome, and the reagents are not commer- cially ...
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Diagnostic Potential of Monoclonal Antibodies Specific to the Unique O-Antigen of Multidrug-Resistant Epidemic Escherichia coli Clone ST131-O25b:H4
... O25b antigen in order to de- velop quick diagnostic ...O25a O-antigen had been replaced by O-acetyl- rhamnose in the O25b repeating ...
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Use of O Antigen Gene Cluster Specific PCRs for the Identification and O Genotyping of Yersinia pseudotuberculosis and Yersinia pestis
... the O-antigen gene clusters in these two organisms identified two regions that were used to specifically identify ...the O-antigen gene clusters of the 21 known ...and ...
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