4 Oligonucleotide primer sequences used for PCR
Gene detection with synthetic oligonucleotide sequences
... OF PCR PRODUCTS Several attempts were made to clone the PCR products of genomic amplification using the short CG-rich oligos directly into vectors, in order to facilitate the sequencing of the PCR ...
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FASTPCR software for PCR, in silico PCR, and oligonucleotide assembly and analysis
... and Oligonucleotide Assembly and Analysis Ruslan Kalendar, David Lee, and Alan ...for PCR primer and probe design. It also predicts oligonucleotide properties based on experimental studies of ...
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Fast comparison of DNA sequences by oligonucleotide profiling
... generate sequences expected to occur very rarely by chance, are ...Consequently, 4 k different decimal numbers serve to represent all possible nucleotide sequences of size ...are used as ...
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Identification of Potential Methylation Regions of the Smad4 mRNA and Determining Primer Sequences for MS-PCR with the ‘Methprimer’ Program
... bisulphite sequences for these regions and designing relevant primer sequences of SMAD4 for reverse transcriptase ...Methylation-specific PCR is one of the most commonly utilized methods to ...
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Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences
... DNA from the cell lines KG1, KG1a and MDA-MB-231 and the peripheral blood from a normal volunteer were extracted using the QIAamp DNA blood mini kit (Qiagen, Hilden, Germany), with the added step of digesting the samples ...
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FluDen Primer DB PCR Primer Database for Influenza A and Dengue Virus
... Keywords: PCR, Influenza, Dengue, NetPrimer, FastPCR. INTRODUCTION PCR provides alternative to culture based detection as changing approaches to clinical diagnostics ...of PCR is its ...methods. ...
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Group specific differentiation between high and low risk human papillomavirus genotypes by general primer mediated PCR and two cocktails of oligonucleotide probes
... be used to replace the original GP-PCR probe, which was able to detect a broader spectrum of HPVs, including putative novel high-risk HPVs ...general primer PCR assay combined with three ...
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Computer Programs for PCR Primer Design and Analysis
... base primer will statis- tically occur only once in every 4 16 bases, or about 4 billion bases, which is about the size of the human ...longer primer with its target sequence is an extremely ...
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A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium
... multiplex oligonucleotide ligation reaction, a differ- ent probe pair is included for each marker in the ...singleplex PCR with a universal primer pair, i.e., T7 and T3. The T3 primer is 5′ ...
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Design of an improved set of oligonucleotide primers for genotyping MeCP2tm1.1BirdKO mice by PCR
... a PCR product with either one pair of primer or the other (P5-P6 for the KO, P5-P7 for the ...we used was that genotyping of males frequently gave rise to bands in both sets of PCR reactions, ...
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Combined PCR oligonucleotide ligation assay for rapid detection of Salmonella serovars
... (ELISA)-based oligonucleotide ligation assay (OLA) was ...the PCR-OLA technique was compared with conventional culture and membrane hybrid- ization for the detection of Salmonella ...the PCR-OLA with ...
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Single Step PCR Using (GACA)4 Primer: Utility for Rapid Identification of Dermatophyte Species and Strains
... two PCR-based methods were compared for their abilities to identify 21 dermatophyte isolates obtained from Egyptian patients to the species and strain ...method: PCR amplification, using ITS1 and ITS4 as ...
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Identification of Bordetella pertussis infection by shared primer PCR
... A shared-primer PCR method for the detection of infection was developed by using primers derived from DNA sequences upstream of the structural genes for the porin proteins of Bordetella [r] ...
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Methylation-specific PCR: four steps in primer design
... are sequences inside the CpG island to which transcription factors (TFs) ...consensus sequences containing transcription factor binding ...successfully used by Bozovic et al [22 ] to design primers ...
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Identification of SNPs and INDELS in swine transcribed sequences using short oligonucleotide microarrays
... Our comparison of an analysis performed using divergent tissues (placenta versus fibroblast) demonstrate that this method is not limited to gene expression data obtained from the same tissue or treatment and is capable ...
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Evaluation of methods for oligonucleotide array data via quantitative real-time PCR
... [10] used stand- ard 2 primer PCR followed by radioactive Southern blot ...time PCR machine in our study allowed greater assurances that all amplifications measured were consistent, specific, ...
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PCR Based Genotyping of Mycobacterium tuberculosis with New GC Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers
... a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis ...16-bp sequences in combination with primers sited at the inverted repeats flanking IS6110 allowed differentiation of ...
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Stepwise partially overlapping primer-based PCR for genome walking
... arbitrary primer (walking primer or SWPOP primer) partially overlapping strategy to remove this type of undesired ...SWPOP primer in use anneal to the former SWPOP primer com- ...
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Detection and analysis of diverse herpesviral species by consensus primer PCR
... In the present study, we observed that the DNA polymerase- coding region flanked by conserved motifs A and B (Fig. 1A) is unique in every herpesviral species examined (Fig. 2 and 3). Importantly, strain-to-strain ...
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Robust SNP genotyping by multiplex PCR and arrayed primer extension
... regulatory sequences for that individual patient [1,2], rap- idly and at the ...Arrayed primer extension (APEX [8,9]) is a minise- quencing microarray assay based on a two-dimensional array of ...
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