ORF3 protein
The PSAP Motif within the ORF3 Protein of an Avian Strain of the Hepatitis E Virus Is Not Critical for Viral Infectivity In Vivo but Plays a Role in Virus Release
... The ORF3 protein is dispensable for virus infectivity in vitro but is essential for establishing viral infection in vivo as demon- strated in rhesus macaques and pigs (13, ...The ORF3 protein ...
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Adenovirus E4 ORF3 Protein Inhibits the Interferon-Mediated Antiviral Response
... E4 ORF3 protein is required for efficient Ad DNA ...E4 ORF3 or E4 ORF6 rescues viral genome replication to levels compara- ble to that of wild-type virus (4, ...E4 ORF3 is neces- sary for ...
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The Adenovirus E4 ORF3 Protein Binds and Reorganizes the TRIM Family Member Transcriptional Intermediary Factor 1 Alpha
... (E4 ORF3) protein is its reorganization of promyelocytic leukemia (PML) protein nuclear ...E4 ORF3 into tracks that eventually surround viral replication ...E4 ORF3, yet its cause and ...
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Proteomic Analysis of Ubiquitin-Like Posttranslational Modifications Induced by the Adenovirus E4-ORF3 Protein
... E4- ORF3-induced sumoylation, since we previously found a strict correlation between relocalization of SUMO proteins into E4- ORF3-containing nuclear tracks and the ability of E4-ORF3 to direct ...
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The adenovirus E4 ORF3 protein stimulates SUMOylation of general transcription factor TFII I to direct proteasomal degradation
... TFII-I protein levels were partially restored by MG132 or epoxomicin treatment and higher-molecular- weight TFII-I species were evident, consistent with the stabiliza- tion of TFII-I-ubiquitin conjugates ...E4- ...
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ORF3 Protein of Hepatitis E Virus Is Not Required for Replication, Virion Assembly, or Infection of Hepatoma Cells In Vitro
... ORF2 protein or ORF3 protein (5, 13) replicated in cultured cells, but a mutant lacking ORF3 protein was unable to infect ma- caques ...of ORF3 protein might be to enable ...
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The ORF3 Protein of Hepatitis E Virus Delays Degradation of Activated Growth Factor Receptors by Interacting with CIN85 and Blocking Formation of the Cbl-CIN85 Complex
... the ORF3-EGFP or the ⌬ D1-ORF3-EGFP fu- sion protein using pEGFP as a ...the ORF3-EGFP or ⌬ D1-ORF3-EGFP fusion protein but not from cells expressing ...the ORF3-EGFP or ⌬ ...
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Interaction of the Adenovirus Type 5 E4 Orf3 Protein with Promyelocytic Leukemia Protein Isoform II Is Required for ND10 Disruption
... The Orf3 coding sequence was cloned into pGEX-2T (Amersham) to produce a glutathione S-transferase (GST) ...GST-tagged Orf3 protein and GST alone were each expressed in Escherichia coli cells grown ...
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The ORF3 Protein of Porcine Circovirus Type 2 Is Involved in Viral Pathogenesis In Vivo
... identified protein, ORF3, was not essential for PCV2 replication in cultured PK15 cells and played a major role in virus-induced ...the ORF3 protein in viral pathogenesis in vivo, we ...
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Cellular Proteins PML and Daxx Mediate an Innate Antiviral Defense Antagonized by the Adenovirus E4 ORF3 Protein
... E4 ORF3 mutant virus during the antiviral state induced by either IFN- ␣ or IFN- ␥ to an extent comparable to that observed in unstimulated cells ...Daxx protein plays a critical role in inhibiting mutant ...
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Self-Association and Mapping of the Interaction Domain of Hepatitis E Virus ORF3 Protein
... truncated ORF3 proteins, we have shown, using the in vivo yeast two-hybrid assay and in vitro immunoprecipitations, that this 43-amino-acid interac- tion domain of pORF3 is capable of ...the ORF3 ...
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Impact of the Adenoviral E4 Orf3 Protein on the Activity and Posttranslational Modification of p53
... E4 Orf3 protein inac- tivates p53, even when p53 accumulates to high concentrations in the absence of the E1B 55-kDa protein ...⌬ Orf3 and ⌬ E1B p53 popula- tions to one another than to that ...
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Distinct Roles of the Adenovirus E4 ORF3 Protein in Viral DNA Replication and Inhibition of Genome Concatenation
... E4 ORF3 functions do not correlate with association of known binding ...E4 ORF3 protein has been shown to interact with the Ad E1B 55K protein during viral infection and in transformed cells ...
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The Adenovirus E4 ORF3 Protein Stimulates SUMOylation of General Transcription Factor TFII I to Direct Proteasomal Degradation
... (E4-ORF3) protein forms unique inclusions throughout the nuclei of infected cells and inhibits the antiviral Mre11-Rad50-Nbs1 DNA repair complex through ...TFII-I protein levels. We also show that ...
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The ORF3 Protein of Porcine Circovirus Type 2 Interacts with Porcine Ubiquitin E3 Ligase Pirh2 and Facilitates p53 Expression in Viral Infection
... PCV2 ORF3 protein. This protein belongs to the class of E3 ubiquitin li- gases with a RING-H2 motif (17), which causes the ubiquiti- nation and degradation of p53 and impairs the growth-sup- pressing ...
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The 41-Amino-Acid C-Terminal Region of the Hepatitis E Virus ORF3 Protein Interacts with Bikunin, a Kunitz-Type Serine Protease Inhibitor
... of ORF3 with bikunin, dual-labeling experiments were conducted ...showing ORF3 protein interaction with full-length ...showing ORF3-ORF3 (19) and ORF3-AMBP (39) interactions were ...
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Two Nonoverlapping Domains on the Norwalk Virus Open Reading Frame 3 (ORF3) Protein Are Involved in the Formation of the Phosphorylated 35K Protein and in ORF3-Capsid Protein Interactions
... the ORF3 protein is unphosphorylated and the larger forms of the ORF3 protein are ...the ORF3 protein in NV virions partially purified from NV-infected volunteers suggests that ...
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Relocalization of the Mre11-Rad50-Nbs1 Complex by the Adenovirus E4 ORF3 Protein Is Required for Viral Replication
... anti-E4 ORF3 rat monoclonal antibody (6A-11; gift from Thomas Dobner, Regensberg Universi- tat), anti-DNA binding protein (DBP) monoclonal antibody (gift from Arnold Levine, Princeton University), anti-DBP ...
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The Hepatitis E Virus ORF3 Protein Modulates Epidermal Growth Factor Receptor Trafficking, STAT3 Translocation, and the Acute-Phase Response
... in ORF3-expressing ...in ORF3-expressing cells, the EGFR-ligand complex was not found in the Rab7-positive compartment ...in ORF3-expressing ...and ORF3-expressing cells ...
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The ORF3 protein of hepatitis E virus is a phosphoprotein that associates with the cytoskeleton.
... In its N-terminal half, pORF3 appears to contain two hy- drophobic domains which have been proposed to constitute either transmembrane segments or a signal sequence followed by a transmembrane region (36). Earlier we had ...
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