Organ culture
Organ culture of seminiferous tubules using a modified soft agar culture system
... cells, organ culture has also been used to generate fertile sperm ...of organ culture which results in loss of sperm- atogonial stem cells (SSCs) caused by hypoxia [16, ...during ...
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Modulation of inflammatory mediators in the trigeminal ganglion by botulinum neurotoxin type A: an organ culture study
... Recently, we asked if a chronic inflammation elicited with injection into the TMJ of CFA [32] or CGRP [33], of CFA on the dura mater (Lukács M, Haanes KA, Majláth Z, Tajti J, Vécsei L, Warfvinge K, Edvinsson L. Exposure ...
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An organ culture system to model early degenerative changes of the intervertebral disc
... rat organ cul- ture described here, despite several similarities with the human pathological state, lacks true mechanical loading, although medium osmolarity is raised to mimic loading conditions and to counteract ...
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Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes
... an organ culture system that maintains the development of the different fetal testis cell types, to evaluate the effects of various endocrine disruptors on gametogenesis and steroidogenesis in the ...this ...
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Short Term Organ Culture of Mouse Ovary in the Medium Supplemented with Bone Morphogenetic Protein 15 and Follicle Stimulating Hormone: A Morphological, Hormonal and Molecular Study
... ovarian organ culture with BMP15 and FSH yields the higher percentages of follicular development to antral stage; moreover, it could cause an increase in size and function of ovaries by production of high ...
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Increased sensitivity of microbiological testing of cornea organ culture medium by additional resin treatment
... results After 20 min of RESEP treatment, 100% (n=9) of streptomycin, 100% (n=9) of amphotericin B and 99.7% (n=9) of penicillin G were eliminated. In group A , all microorganisms were detected within 3 days of incubation ...
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Replication of Varicella-Zoster Virus in Human Skin Organ Culture
... Alternatives to studying VZV in skin implanted in SCID-hu mice are organotypic raft cultures and skin organ culture (SOC). There are few reports of organ cultures being used to study infectious ...
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Modeling of Human Cytomegalovirus Maternal-Fetal Transmission in a Novel Decidual Organ Culture
... of organ culture infection was consistently observed in more than 80 decidual tissues ob- tained from different subjects and could be similarly monitored following infection with the endotheliotropic strain ...
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A high throughput template for optimizing Drosophila organ culture with response surface methods
... model organ for molecular developmental ...Drosophila organ culture techniques are becoming crucial for further ...a culture medium for wing ...optimized culture system that highlights ...
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Aspects of morphogenesis and cytodifferentiation of endoderm offered by organ culture techniques
... Int I De\' Bioi 38 267 272 (1994) 267 Review Aspects of morphogenesis and cytodifferentiation of endoderm offered by organ culture techniques TAKEO MIZUNO' Department of Biology, Faculty of Pharmaceut[.] ...
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Biological Effects of Clinically Relevant CoCr Nanoparticles in the Dura Mater: An Organ Culture Study
... the organ culture conditioned medium for a period of seven days after the exposure of the organ culture to 5 and 50 µm 3 CoCr nanoparticles per epithelial ...
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Non-destructive monitoring of viability in an ex vivo organ culture model of osteochondral tissue
... Determination of the viability of cells in tissues in organ culture can be complex, since there are two modes of cell death – necrosis and apoptosis. Necrotic cells lose their membrane integrity in an ...
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Development of fetal thymocytes in organ culture: effect of corticosteroids
... Inl I Dc\' Hiul 36 573 577 (1992) 573 Shor/ COIl/rilm/ioll Development of fetal thymocytes in organ culture effect of corticosteroids MARGOT A SKINNER", LOUISE MOFFATT' and STEPHEN J M SKINNER' 1Depar[.] ...
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Stimulation of Gastrin Secretion and Synthesis in Antral Organ Culture
... The purpose of the present study was to examine stimulation of gastrin release and the synthesis of gastrin directly by measurement of incorporation of [ 3 H]tryptophan into gastrin in rat antral mucosal explants ...
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Organ culture of mucosal biopsies of human small intestine
... In vitro experiments of small intestinal mucosal function and metabolism utilizing excised tissue have been limited to a few hours by rapid epithelial cell necrosis which occurs with current incubation methods. We ...
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Transforming growth factor beta2 promotes the formation of the mouse cochleovestibular ganglion in organ culture
... Since Tgf β 2 was expressed in the anteroventrolateral wall of the OV, we assumed that TGF β 2 might play some role in CVG develop- ment. To test this hypothesis, we cultured the otic vesicles of E10.5 mouse embryos in ...
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Bone resorption in organ culture: inhibition by the divalent cation ionophores A23187 and X 537A
... In experiment A, after 24 h of incubation, a 200-,ul aliquot of medium was removed for calcium determination, and 200 ,M of control medium was added to the control group 0, and the group[r] ...
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Cellular and Tissue Responses to Implant Materials: Development of a Novel Organ Culture Model
... serum-free culture, coating of Bioglass ...Tissue culture polystyrene is known to have enhanced fibronectin binding properties (Bentley and Klebe, 1985) and, although these variables have not been explored ...
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Cardiotonic agent milrinone stimulates resorption in rodent bone organ culture
... We find similarities between milrinone and thyroxine actions on bone, because prostaglandin production was crucial for the effects of both agents in calvaria but not in limb bones, and n[r] ...
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Replication of murine paramyxoviruses in hamster tracheal organ culture and comparison with standard tissue culture methods
... Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tr[r] ...
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