Organotypic brain slice culture
An organotypic slice culture model of chronic white matter injury with maturation arrest of oligodendrocyte progenitors
... rat brain and the slice cul- ...in culture. To determine the effect of slice atrophy on PDGFRa cell density measurements, we quantified mean white matter ...each slice, white matter ...
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Organotypic brain slice cultures to model neurodegenerative proteinopathies
... S positive tau lending their use to screen for anti- aggregation therapeutics [59]. Additionally, synergistic and differential effects of Aβ and tau on dendritic simplifica- tion and synapse loss have been identified in ...
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Original Article Implantation of glioblastoma spheroids into organotypic brain slice cultures as a model for investigating effects of irradiation: a proof of concept
... malignant brain tumor with an overall survival of only ...and brain tissue are highly needed in order to develop novel efficient therapeutic ...rat brain slice culture model comprising ...
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Dexmedetomidine is neuroprotective in an in vitro model for traumatic brain injury
... for organotypic slice cultures because this model allows easy access to in vitro manipulation of ner- vous tissue and yet mimics closely the in vivo state of the tissue with respect to morphological and ...
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HIV-1 Tat activates indoleamine 2,3 dioxygenase in murine organotypic hippocampal slice cultures in a p38 mitogen-activated protein kinase-dependent manner
... of brain cells, OHSCs have the advantage of preserving the cellular and con- nective organization as well as several fundamental in vivo-like characteristics such as glial-neuronal interac- tions ...term ...
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Organotypic slice culture model demonstrates inter-neuronal spreading of alpha-synuclein aggregates
... vivo brain tissue culture model that can serve as a translational step between cell-based in vitro and in vivo models for synu- ...hippocampal slice culture that comprises a well suited tissue ...
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A novel and generalizable organotypic slice platform to evaluate stem cell potential for targeting pediatric brain tumors
... Beside ethical and immunologic concerns associated with the use of fetal-derived tissue as a stem cell source, the problem is that lack of uniform platform in current stem cell research leads to an inconsistent efficacy. ...
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Cancer stem cells from a rare form of glioblastoma multiforme involving the neurogenic ventricular wall
... Results: The patient ’ s MRI revealed a heterogeneous mass with associated edema, involving the left subventricular zone. Histological examination of the tumor established it as being a high-grade glial neoplasm, ...
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Synaptophysin depletion and intraneuronal Aβ in organotypic hippocampal slice cultures from huAPP transgenic mice
... whole brain slices from adult APP_SDI mice; a failure to develop plaques, but appearance of atypical intraneur- onal Aβ staining ...in slice culture is more prone to retain Aβ within cells than in ...
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Borna Disease Virus Replication in Organotypic Hippocampal Slice Cultures from Rats Results in Selective Damage of Dentate Granule Cells
... cell culture, including in primary neuronal cells ...whether organotypic hippocampal slice cultures from newborn rats could provide a suitable system to study BDV- induced pathology in this ...
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Central Administration of Lipopolysaccharide Induces Depressive-like Behavior in Vivo and Activates Brain Indoleamine 2,3 Dioxygenase In Murine Organotypic Hippocampal Slice Cultures
... murine organotypic culture system serve as a reliable in situ model for investigating mechanistic aspects of the depressive effects of inflammation? (3) Does this in situ model of OHSCs respond like primary ...
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Organotypic culture of acinar cells for the study of pancreatic cancer initiation
... 3D culture of acinar cells are the difficulty to extend the culture for a period of time long enough to allow a long-term experiment and the significant stress and variability introduced by isolation ...
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PubMedCentral-PMC5135012.pdf
... Different early human neural stem/progenitor cell types, including neuroepithelial-like cells, have been derived from hPSCs and the developing human brain (Conti and Cattaneo, 2010; Edri et al., 2015; Elkabetz et ...
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Docosahexaenoic acid (DHA): a modulator of microglia activity and dendritic spine morphology
... hippocampal slice cultures and treatment Organotypic hippocampal slice cultures were prepared as previously described ...the slice cultures were prepared from P6-8 transgenic mice that ...
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The brain slice method for studying drug distribution in the CNS
... Each cassette of compounds is prepared individually, ex tempore, in scintillation vials (20 ml glass vials with screw lids; one vial per rat or mouse brain). Initially, the required volume of stock drug solution ...
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The metastatic infiltration at the metastasis/brain parenchyma- interface is very heterogeneous and has a significant impact on survival in a prospective study
... of brain metastasis without standardized reports of the adjacent brain tissue is still the clinical ...ex-vivo organotypic brain slice coculture model ...adjacent brain ...
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Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System.
... Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach ...
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MRI brain nuclei segmentation and evaluation of segmented nuclei with BET and BSE
... abnormal brain MR images collected from IBSR, Diagnostic centres (Barnard Institute of radiology, Bharat scans and Sri Ramachandra diagnostic centre are shown in Figures-8(a), 9(a) and ...MR brain images, ...
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Akt dependent Pp2a activity is required for epidermal barrier formation during late embryonic development
... keratinocyte organotypic cultures were grown on de-epidermized dermis at the air-liquid interface for 5 and 10 days (Baden and Kubilus, 1983; Marjukka et ...the organotypic cultures whole mount with ...
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Human conjunctival epithelial precursor cells and their progeny in 3D organotypic culture
... p63 expression (Fig. 1A) was exclusively confined to the nucleus of cells of the basal layer of the conjunctiva. 5% ± 2.2 % of basal epithelial cells were cycling, (positive immunostaining for Ki-67, Fig. 1B). ...
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