organotypic cultures
Human Papillomavirus E6E7-Mediated Adenovirus Cell Killing: Selectivity of Mutant Adenovirus Replication in Organotypic Cultures of Human Keratinocytes
... in organotypic cultures of primary human keratinocytes (hereafter referred to as raft ...submerged cultures, because (i) commonly em- ployed submerged cultures may have different properties ...
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Type I TARPs promote dendritic growth of early postnatal neocortical pyramidal cells in organotypic cultures
... The ionotropic α -amino-3-hydroxy-5-methyl-4-isoxazole propionate glutamate receptors (AMPARs) have been implicated in the establishment of dendritic architecture. The transmembrane AMPA receptor regulatory proteins ...
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NADPH oxidase mediates β-amyloid peptide-induced activation of ERK in hippocampal organotypic cultures
... in organotypic hippoc- ampal cultures ...treated organotypic cultures (Figure ...the organotypic cultures with nicotine did not appear to activate ERK in the cell bodies of the ...
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Cytokine inflammatory threat, but not LPS one, shortens GABAergic synaptic currents in the mouse spinal cord organotypic cultures
... spinal organotypic cultures to focus on the interplay be- tween local inflammation and the dynamics of GABAergic currents, whose altered decay may represent a subtle alter- ation able to trigger neuronal ...
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Activation of Adenovirus Early Promoters and Lytic Phase in Differentiated Strata of Organotypic Cultures of Human Keratinocytes
... The cultures were harvested at 24, 48, or 72 h post-adenovirus infection ...raft cultures of PHK or SiHa ...raft cultures that were refractory to adenovirus infection (Banerjee et ...
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Flavivirus Infection of Ixodes scapularis (Black Legged Tick) Ex Vivo Organotypic Cultures and Applications for Disease Control
... This study provides a foundation for using individual tick organs in cultures to study TBFV biology. However, variables exist that may affect organ viability and TBFV repli- cation in organs, such as bloodfeeding ...
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Organotypic brain slices: a model to study the neurovascular unit micro-environment in epilepsies
... Figure 1 Blood vessel responses to seizure-like activity in organotypic hippocampal culture. (A) Schematic diagram of a blood vessel during the angiogenic process. Angiogenesis is the formation of a new blood ...
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Indomethacin-loaded lipid-core nanocapsules reduce the damage triggered by Aβ1-42 in Alzheimer’s disease models
... animals were killed by decapitation, the brains were removed, the hippocampi were isolated, and transverse hippocampal slices (400 µ m thickness) were prepared by using a McIlwain tissue chopper (Mickle Laboratory ...
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Crosstalk between macrophages and astrocytes affects proliferation, reactive phenotype and inflammatory response, suggesting a role during reactive gliosis following spinal cord injury
... cord organotypic cultures exposed to conditioned medium from pro-inflammatory M1 macrophages showed increased glial proliferation, which was further confirmed though the use of primary ...and ...
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Zika virus infects human testicular tissue and germ cells
... of organotypic cultures, we demonstrated here that ZIKV replicates in human testicular tissue and infects a broad range of cell types, including germ cells, which we also identified as infected in semen ...
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Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure
... system in the control of RGC death through a control of neuroinflammation in the retina is further underscored by the observed up-regulation of this system in retinal organotypic cultures exposed either to ...
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A rapid and sensitive assay of intercellular coupling by voltage imaging of gap junction networks
... Here, we used cochlear organotypic cultures to unravel the potential of Vf2.1.Cl, a member of the novel VoltageFluor (VF) family of fluorescent sensors [18]. VF dyes detect voltage changes by modulation of ...
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Squamous epitheliotropism of Enterovirus A71 in human epidermis and oral mucosa
... mucosa organotypic cultures derived from the prepuce and lip biopsies, focal viral antigens and viral RNA were localized to cytoplasm of epidermal and mucosal squamous cells as early as 2 days ...
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Extensive tissue regenerative capacity of neonatal human keratinocyte stem cells and their progeny
... proliferate better on these ECMs, although LN-10/11 recruited more keratinocytes into proliferation com- pared with collagen IV (Figure 5b). Daily microscopic analysis of both fractions of cells plated on LN-10/11 ...
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Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation
... Neuroinflammation is a common process in several neurological diseases, including MS where it leads to demyelination and axonal damage. To assess how methylthioadenosine might affect demyelination/remyeli- nation under ...
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Chronic brain inflammation leads to a decline in hippocampal NMDA-R1 receptors
... Baskys A, Adamchik Y: Neuroprotective effects of extracellular glutamate are absent in hippocampal organotypic cultures treated with the amyloid peptide Abeta (25–35). Bi H, Sze CI: N-[r] ...
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Human Papillomavirus Type 8 E6 Oncoprotein Inhibits Transcription of the PDZ Protein Syntenin-2
... in organotypic skin cultures of HPV8 E6-positive ...for organotypic skin cultures on a collagen-based ...E7 organotypic cultures regenerated a normal epithelium with all ...
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In Vitro Replication of Chelonid Herpesvirus 5 in Organotypic Skin Cultures from Hawaiian Green Turtles (Chelonia mydas)
... matrix. Organotypic cultures have been used to look at development of HSV (62), human CMV (HCMV) (63), and EBV (56), but none have been used to examine virus morphogenesis at the ultrastructural ...cell ...
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Proper patterning of the optic fissure requires the sequential activity of BMP7 and SHH
... BMP7 addition to organotypic cultures of optic vesicles from Bmp7–/– embryos rescues Pax2 expression in the ventral region, while follistatin, a BMP7 antagonist, prevents it in early, bu[r] ...
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Reconstruction of Human Papillomavirus Type 16-Mediated Early-Stage Neoplasia Implicates E6/E7 Deregulation and the Loss of Contact Inhibition in Neoplastic Progression
... seen between E6 and E7 levels and cell growth at subconfluence (Fig. 3C), when the cells reached confluence (as occurs during the raft establishment), all of the HSIL-like cell lines showed an eleva- tion in E6 and E7, ...
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