outer membrane vesicles
Helicobacter pylori outer membrane vesicles involvement in the infection development and Helicobacter pylori-related diseases
... vesiculation Outer membrane vesicles are defined as 20–300 nm blebs, which are naturally secreted by Gram-negative bacteria mainly during their logarithmic phase of growth although, Gram-positive ...
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Immunogenicity of Nontypeable Haemophilus influenzae Outer Membrane Vesicles and Protective Ability in the Chinchilla Model of Otitis Media
... of outer membrane vesicles from Gram-negative bacteria, including those of NTHi (24, 25, 32, ...released outer membrane vesicles from culture supernatants of our NTHi strains ...
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The lytic mechanism of Escherichia coli α hemolysin associated to outer membrane vesicles
... Alpha-hemolysin (HlyA) is an extracellular toxin secreted by Escherichia coli, targeting to plasma membranes of eukaryotic cells. Recently it was found that this toxin is released to external media associated to ...
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Protection against neonatal enteric colibacillosis employing E Coli derived outer membrane vesicles in formulation and without vitamin D3
... In this report ETEC outer membrane vesicles was pre- pared and tested alone or in formulation with avD3 as a new candidate for colibacillosis vaccine. Immuniza- tion of dam mice induced serum and ...
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Surface Exposure and Packing of Lipoproteins into Outer Membrane Vesicles Are Coupled Processes in Bacteroides
... Our new data set with annotations and predicted functions and locations for proteins in all fractions is provided as Table S1 in the supplemental material. We confirmed our previous findings showing an enrichment of ...
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Evaluation of antibody responses to outer membrane vesicles (OMVs) and killed whole cell of Vibrio cholerae O1 El Tor in immunized mice
... V. cholerae OMVs. In the present study, the immu- nogenic performance of OMV in a clinical V. choler- ae strain (VC492) was evaluated. Extraction method with minor modifications was considered a useful tool for OMVs ...
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Comparative study of pathogenic and non pathogenic Escherichia coli outer membrane vesicles and prediction of host interactions with TLR signaling pathways
... One of the main bacterial virulence factors is LPS that is present as part of the OMV structure. LPS also plays an important role in the OMV biogenesis [26]. Our study showed that there are different levels of LPS in the ...
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Bacterial outer membrane vesicles, a potential vaccine candidate in interactions with host cells based
... fragilis: Bacteroides fragilis; BCR: B cell receptor; BMDM: Bone marrow-derived macrophages; BPI: Bactericidal/permeability-increasing protein; CD: Cluster Of Differentiation; CNF1: Cytotoxic necrotizing factor type 1; ...
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Outer Membrane Vesicles Induce Immune Responses to Virulence Proteins and Protect against Colonization by Enterotoxigenic Escherichia coli
... form outer membrane vesicles (OMV), which often contain one or more virulence ...in outer membrane vesicles of ETEC, we sought to examine the immunogenicity and protective ...
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Neisseria meningitidis Native Outer Membrane Vesicles Containing Different Lipopolysaccharide Glycoforms as Adjuvants for Meningococcal and Nonmeningococcal Antigens
... or outer membrane vesicles (OMVs) (4, ...mainly outer mem- brane proteins, lipoproteins, and lipopolysaccharide (LPS), some of which can induce protective immune ...other outer mem- ...
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Inflammasome Activation by Bacterial Outer Membrane Vesicles Requires Guanylate Binding Proteins
... ABSTRACT The Gram-negative bacterial cell wall component lipopolysaccharide (LPS) is recognized by the noncanonical inflammasome protein caspase-11 in the cy- tosol of infected host cells and thereby prompts an ...
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Bacterial outer membrane vesicles and vaccine applications
... Summary: Vaccines based on outer membrane vesicles (OMV) were developed more than 20 years ago against Neisseria meningitidis serogroup B. These nano- sized structures exhibit remarkable potential ...
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Preferential Packing of Acidic Glycosidases and Proteases into Bacteroides Outer Membrane Vesicles
... ABSTRACT Outer membrane vesicles (OMV) are spherical membranous structures released from the outer membrane (OM) of Gram-negative ...
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Phase I Safety and Immunogenicity Study of a Candidate Meningococcal Disease Vaccine Based on Neisseria lactamica Outer Membrane Vesicles
... There is great interest in the development of vaccines for protection against serogroup B meningococcal disease, and vaccines based on outer membrane vesicles (OMVs) have been assessed in efficacy ...
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LPS Remodeling Triggers Formation of Outer Membrane Vesicles in Salmonella
... ABSTRACT Outer membrane vesicles (OMV) are proposed to mediate multiple functions during pathogenesis and ...the outer membrane (OM) and subsequent OMV ...
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Antibiotic treatment modulates protein components of cytotoxic outer membrane vesicles of multidrug-resistant clinical strain, Acinetobacter baumannii DU202
... cal assistance from Young In Frontier, Inc. (Seoul, Korea). Three injections were applied at intervals of 2 weeks, and blood was collected 1 week after the final injection. At 2 weeks after the third injection, serum was ...
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Studies on the synergistic protective activity of cancer antigens associated to engineered bacterial Outer Membrane Vesicles
... Purified OMVs or TEXs (1µg or 5µg protein content) were suspended in SDS- PAGE loading buffer (Bio-Rad) and were then separated on a SDS-PAGE 4- 12% polyacrylamide gel (Thermo Fisher Scientific). Proteins separated by ...
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The association between Fusobacterium nucleatum Outer Membrane Vesicles and Colonic Cancer
... phospholipid, outer membrane proteins, and lipooligosaccharides in addition to content from periplasmic proteins and cell wall components that are trapped in the OMV lumen (Lee et ...
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Escherichia coli-derived outer membrane vesicles are genotoxic to human enterocyte-like cells
... fluorescent, membrane-intercalating dye 3,3′-dioctadecyloxacarbocya- nine perchlorate (DiO) we were able to show that vesicles entered the cells, irrespective of whether they were from attenuated laboratory ...
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A Critical Threshold of Meningococcal Factor H Binding Protein Expression Is Required for Increased Breadth of Protective Antibodies Elicited by Native Outer Membrane Vesicle Vaccines
... Native outer membrane vesicles (NOMV) (not detergent treated), which are prepared from recombinant strains with attenuated endotoxin activity and overexpressed factor H binding protein (fHbp), ...
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