Overlap concentration for nucleic acid solutions
Calculating Nucleic Acid or Protein Concentration Using the GloMax Multi+ Microplate Instrument
... of nucleic acid samples is that proteins and reagents commonly used in the preparation of nucleic acids also absorb light at 260 nm and can lead to falsely elevated concentration ...stranded ...
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Peptide Nucleic Acid Microarrays
... mix solutions and each of the four monomers (A, G, C, ...monomer solutions are pipetted, one at a time, into the respective ...monomer solutions that can be worked with during a run is not limited to ...
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How To Make A Nucleic Acid
... The concentration of the solutions of expensive PNA monomers, activator (HATU), and base (DIEA) was adjusted on the basis of the loading capacity and amount of used resin and the solutions were ...
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An Introduction to Peptide Nucleic Acid
... PNA/nucleic acid duplex leaves the T m largely independent of salt concentration (Figure 3) ...a nucleic acid target despite the presence of competing secondary structure ...
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Search for a Prion-Specific Nucleic Acid
... 5A). After an initial decrease up to about 50 kJ/m 2 , the curves nearly level off. We observed two effects that might be responsible for the nonlinearity. First, the solutions exhibited a fairly high optical ...
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Polysaccharides as initiators of nucleic acid polymerization
... NA concentration is increased in the PU-NA complexes in the subsequent processes of solution and repeated dehydration of the complexes with the mixture of nucleotides, as it is demonstrated in Table ...
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PNA Biosensors for Nucleic Acid Detection
... The goal is to convert the biological recognition event into a useful electrical signal, proportional to the concentration of the target analyte. By combining the sample handling and measurement steps, biosensors ...
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Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH
... 1st step PCR: 94 °C 3 min, (94 °C 30 s, 55 °C 30 s, 72 °C 1 min 40 s) for 40 cycles, 72 °C 5 min; 2nd step PCR: 94 °C 3 min, (94 °C 30 s, 65 °C 20 s, 55 °C 50 s, 72 °C 15 s) for 30 cycles. Sensitivity was assessed ...
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Evolution of Diversely Functionalized Nucleic Acid Polymers
... I would like to thank all past and present Liu lab members with whom I had the great fortune to overlap. In particular, Jia Niu was my primary mentor in my first two years, and I thank him for teaching me ...
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Advances in single molecule nucleic acid sequencing
... sufficient overlap during shotgun sequencing assembly, up to 720 uninterrupted reagent exchange steps are necessary over a series of several days, in order to obtain all of the information needed to reconstruct an ...
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Analysis, Design, and Construction of Nucleic Acid Devices
... 5.1 Abstract Given the previously developed tools for the analysis and design of nucleic acid secondary structures, the challenge is to parlay these techniques into useful DNA systems. To this end, we must ...
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Multiplexed Nucleic Acid Programmable Protein Arrays
... M-NAPPA would be useful in unbiased HT screening studies, such as protein-protein interactions, protein-DNA interactions, discovery of drug binding target as well as (auto)antibody biomarkers for a variety of human ...
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In Vitro Selection of Functional Nucleic Acid Sequences
... Selection Stringency The first two or three SELEX rounds, when individual sequences are not highly represented, should be performed under low stringency conditions to ensure the recovery of rare functional sequences. ...
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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... [62] nucleic acid capture ...electrochemical nucleic acids probe made of PNA which exhibits high sensitivity and specificity when challenged with heterogeneous samples of ...
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On nucleic acid photochemistry
... General features. Ultraviolet action spectra for the formation of thymine dimer in E. coli DNA are shown in figs. Qual- itatively, the spectra may be interpreted to mea[r] ...
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Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes (US)
... activated only when the two strands bind to the target analyte reconstituting the catalytic core. The activated enzyme then cleaves the bound substrate generating a signal localized [r] ...
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Nucleic Acid Electrophoresis Cell
... 3. Set Model 583 Gel Dryer to sequencing cycle. 30 minutes at 80 °C should suffice for drying thin low percent gels, if the applied vacuum is above 28 inches of mercury or 125 torr. Refer to the dryer’s instruction ...
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Report in Nucleic Acid[1]
... Orcinol Test Also called Bial's test, is a chemical test for the presence of those sugars or their derivatives which can form furfural upon heating in acidic medium. Furfural formed from pentoses reacts further with ...
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Protein-nucleic acid interactions
... Classes of DNA bending proteins Williams & Maher (2000) “Electrostatic mechanisms of DNA deformation.” Ann. Rev. Biophys. Biomol. Struct. 29, 497-521 Class-1 bending proteins such as TBP bind the DNA minor groove, unwind ...
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