P. berghei
Expression of full-length Plasmodium falciparum P48/45 in P. berghei blood stages: A method to express and evaluate vaccine antigens
... (D) Immuno-fluorescence analyses of Pfs48/45 expression in purified schizonts of Pfs48/45@PbMSP1 (1807cl2), and the reference parent P. berghei GIMO line (i.e. WT; 1596cl1). Fixed parasites were stained with ...
6
Unlike the synchronous <em>Plasmodium falciparum</em> and <em>P. chabaudi</em> infection, the <em>P. berghei</em> and <em>P. yoelii</em> asynchronous infections are not affected by melatonin
... in [Ca 2+ ] i , (205 ± 32.85 nM, n = 3) is observed. In accordance with these results, panel b shows that monensin, a Na + /H + ionophore, that can induce the release of Ca 2+ from an acidic pool in other Plasmodia ...
10
Antimalarial properties of n 3 and n 6 polyunsaturated fatty acids: in vitro effects on Plasmodium falciparum and in vivo effects on P berghei
... The polyunsaturated fatty acids docosahexaenoic acid (C22:6,n-3), eicosapentaenoic acid, arachidonic acid, and linoleic acid caused marked in vitro growth inhibition of Plasmodium falciparum, assessed by a radiometric ...
8
COMPARISON OF PATHOGENESIS OF P. BERGHEI INFECTION IN MOUSE AND RAT MODELS
... Lastly, the high survival rate in the rat survival model could be due to the probable immunization against lethal P. berghei infection with a complete resolution of its mild parasitemia without any ...
9
Asiatic acid influences parasitaemia reduction and ameliorates malaria anaemia in P berghei infected Sprague–Dawley male rats
... Together with increased suppression of parasitaemia, administration of AA10 dose was associated with improved food and water intake and preserved weight gain compared to both 30CHQ dose [r] ...
10
IN VITRO ANTI-PROTOZOAL ASSESSMENT OF VERNONANTHURA PATENTS EXTRACTS
... of P. berghei ANKA blood stages were performed as described before (Janse & Waters, ...of P. berghei ring forms/young trophozoites were prepared at 1% parasitemia and 1% final erythrocytes ...
6
Malaria infection does not affect the sensitivity of peripheral receptor neurons in Anopheles stephensi
... Our data indicate that infection of An. stephensi with P. berghei does not affect the sensitivity of maxillary palp sensilla of female An. stephensi with regard to the detection of carbon dioxide or ...
8
Isolation of Plasmodium berghei ookinetes in culture using Nycodenz density gradient columns and magnetic isolation
... CD mice were treated with phenylhydrazine two days prior to infection by intra peritoneal inoculation with P. berghei ANKA strain, obtained from a donor mouse between the second and sixth passage from ...
6
IN VIVO ANTIMALARIAL ACTICITY OF METHANOLIC EXTRACT OF YOUNG FRONDS OF PTERIDIUM AQUILINUM L. KUHN IN MICE INFECTED WITH PLASMODIUM BERGHEI
... The results of this study have shown that the methanolic extract of P. aquilinum young frond possesses antimalarial activity as seen in its ability to suppress P. berghei infection in mice. The ...
7
Experimental malaria associated acute respiratory distress syndrome is dependent on the parasite host combination and coincides with normocyte invasion
... with P. berghei NK65-E com- pared to P. berghei NK65-NY ...1). P. berghei NK65- E induced a full spectrum of pathology including an increase in lung weight, extensive alveolar ...
17
Preliminary Study: Neurobehavioural Effects of Nauclea latifolia and Emilia sonchifolia in Mice Infected with Plasmodium berghei berghei
... This study was designed to assess the effect of oral administration of ethanolic extracts of Nauclea latifolia and Emilia sonchifolia in parasitized mice during locomotion, anxiety and fear tests using the Open field ...
10
Mesenchymal stromal cell therapy attenuated lung and kidney injury but not brain damage in experimental cerebral malaria
... In P. berghei-infected mice, spleen damage was observed with activation of lymphocytes in white pulp, increased deposition of malaria pigment in red pulp (single white arrows), and increased number of ...
15
Antiplasmodial potential of the African mistletoe: Agelanthus dodoneifolius Polh and Wiens
... parasite, P. berghei, this parasite is sensitive to ...chloroquine. P. berghei 4 day suppressive test was used to assess the efficacy of our extract by comparison of blood parasitaemia and ...
7
ANTIMALARIAL ACTIVITY OF CRYPTOLEPIS SANGUINOLENTA BASED HERBAL CAPSULES IN PLASMODIUM BERGHEI INFECTED MICE
... Phytochemical screening of the herbal capsules revealed the presence of alkaloids and other phytocompounds. Alkaloids have been implicated in the antiplasmodial activities of many plant extracts 14 . It is speculated ...
5
Mosquito Akirin as a potential antigen for malaria control
... Recombinant AKR from Ae. albopictus was produced as previously reported using an extractive bioconversion process in an aqueous two-phase system supporting Pichia pastoris growth and protein secretion and used on ...
8
Evaluation of antiplasmodial effects of the ethanolic leaf extract of Salacia lehmbachii on Plasmodium berghei infected mice
... On the first day, thirty Swiss albino mice were inoculated with P. berghei infected erythro- cytes. After 72 hrs, the mice were randomly grouped into 5 groups of 6 mice, and treated daily (groups 2-4) for ...
7
Further evidence for an anti inflammatory role of artesunate in experimental cerebral malaria
... A recent study conducted by Linares et al. [11] demon- strated an increase in IFN-γ and TNF mRNA expression in several brain areas of P. berghei-infected mice, including cortex, hippocampus, ...
13
Impact of chrysosplenetin on the pharmacokinetics and anti malarial efficacy of artemisinin against Plasmodium berghei as well as in vitro CYP450 enzymatic activities in rat liver microsome
... Methods: Twenty rats were randomly divided into four groups and received three-day oral doses of ART in absence or presence of CHR (in ratio of 1:0, 1:1, 1:2, and 1:4, respectively). Plasma samples were separately ...
13
Overexpression of Plasmodium berghei ATG8 by Liver Forms Leads to Cumulative Defects in Organelle Dynamics and to Generation of Noninfectious Merozoites
... Plasmodium falciparum infecting human hepatocytes in a chi- meric mouse expresses ATG8 until the merozoite stage. Our previous immunofluorescence assays (IFA) on Plasmodium ber- ghei in hepatoma cells labeled with ...
24
ANTIMALARIAL ACTIVITY OF CALPURNIA AUREA HYDROALCOHOLIC LEAF EXTRACT IN MICE INFECTED WITH PLASMODIUM BERGHEI
... as P. berghei due to the fact that plasmodium species that cause human disease are essentially unable to infect non primate animal models with the exception of a complex immunocompromised mouse model that ...
7