P erk1 2
Extreme loss of immunoreactive p Akt and p Erk1/2 during routine fixation of primary breast cancer
... between core-cut and excision values indicates a sys- tematic difference. At room temperature and without fixation accelerators, formalin, by far the most widely used histopathological fixative, penetrates tissue at ...
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Original Article Expression of G protein-coupled estrogen receptor 30 (GPR30) and extracellular signal-regulated kinase 1/2 (p-ERK1/2) in the endometrioid endometrial cancer and their correlation
... the p-ERK1/2 expression was comparable between EAH and normal endometrium ...increase p-ER- K1/2 expression, stimulate cell proliferation, and inhibit cell apoptosis, which are involved ...
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Neuroprotective effects of bilobalide on cerebral ischemia and reperfusion injury are associated with inhibition of pro-inflammatory mediator production and down-regulation of JNK1/2 and p38 MAPK activation
... for p-ERK1/2 (p: phosphorylated, active form), p-JNK1/2, p-p38 MAPK (1:500 dilution), total ERK1/2, total JNK1/2, and total p38 MAPK (1:1000 dilution, ...
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Caveolin-1 promotes Ewing sarcoma metastasis regulating MMP-9 expression through MAPK/ERK pathway
... IQGAP1 has been involved in invasion [18] and linked to ERK2 modulating its activity [19]. Thus, IQGAP1 could be playing a similar role in ES. First, we checked that all ES cell lines expressed IQGAP1 (Figure 3A). Then, ...
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Inhibiting ERK Activation with CI-1040 Leads to Compensatory Upregulation of Alternate MAPKs and Plasminogen Activator Inhibitor-1 following Subtotal Nephrectomy with No Impact on Kidney Fibrosis
... increased ERK1/2 activation in the glomeruli is an appropriate response to tissue injury and thus inhibition of ERK1/2 may actually impede tissue ...of ERK1/2 by CI-1040 we ...
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HIV-1 Infection Abrogates CD8+ T Cell Mitogen-Activated Protein Kinase Signaling Responses
... abrogated ERK1/2 signaling response indicates the pres- ence of a block in the MAPK ...induces ERK1/2 phosphorylation through activation of PKC, which in turn pos- itively modulates activity ...
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Genistein Attenuates Brain Damage induced by Transient Cerebral Ischemia Through Up-regulation of ERK Activity in Ovariectomized Mice
... with 2% bovine serum albumin (BSA) in ...antibodies p-ERK1/2, ERK1/2, Bcl-2, Bax and β-Tubulin (1:1000, Abcam, Cambridge, United Kingdom), the membrane was incubated with ...
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<p>Ras-ERK1/2 Signaling Promotes The Development Of Osteosarcoma By Regulating H2BK12ac Through CBP</p>
... MG-63 cells were collected after transfection for 48 hrs, and these collected cells were washed once with PBS. The whole- cell lysates were prepared by using RIPA lysis buffer (Beyotime Biotechnology). The concentration ...
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Original Article Sorafenib downregulates ERK/Akt and STAT3 survival pathways and induces apoptosis in a human neuroblastoma cell line
... decreased p-ERK1/2 in response to sorafenib ...both p-ERK and p-Akt may lead to the changes in other survival factors including NF-kB [27], which eventually lead to cell apoptosis seen ...
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Expression of phospho-ERK1/2 and PI3-K in benign and malignant gallbladder lesions and its clinical and pathological correlations
... and 2 with ampullary ...of p-AKT in ...of p-MAPK/p-ERK1/2 or p-AKT in cholangiocarcinoma [7], intra-hepatic cholangiocarci- noma [8], and cholangiocarcinoma [13], but the ...
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Chronic brain inflammation causes a reduction in GluN2A and GluN2B subunits of NMDA receptors and an increase in the phosphorylation of mitogen-activated protein kinases in the hippocampus
... of 2 antibodies from anti-p-Erk1/2 (1:300, Cell Signaling), anti-p-p38 (1:300, Cell Signaling), anti-NeuN (1:2000, Millipore), anti-CD11b (1:1000, Serotec), and anti-glial fibrillary ...
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<p>Dexmedetomidine attenuates the neurotoxicity of propofol toward primary hippocampal neurons in vitro via Erk1/2/CREB/BDNF signaling pathways</p>
... the p-Erk1/2, p-CREB, and BDNF proteins compared with Group C (one-way ANOVA, *P , ...the p-erk1/2, p-creB, and BDnF proteins (one-way anOVa, ...group ...
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Activation of Rac1 by Src dependent phosphorylation of Dock180Y1811 mediates PDGFRα stimulated glioma tumorigenesis in mice and humans
... of p-Erk1/2, but with less reduction of ...stimulate p-Akt, inhibition of Dock180 only partially reduced p-Akt, but attenuated PDGFRα-pro- moted survival and growth of glioma ...
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<p>Knockdown of TRIM37 Promotes Apoptosis and Suppresses Tumor Growth in Gastric Cancer by Inactivation of the ERK1/2 Pathway</p>
... Abcam), ERK1/2 (1:1000, #9102, Cell Signaling Technology [CST]), p-ERK1/2 (1:1000, #9101, CST) and GAPDH (1:2000, #5174, CST) overnight with gentle ...
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Inhibition of extracellular signal-regulated kinase 1/2 signaling has beneficial effects on skeletal muscle in a mouse model of Emery-Dreifuss muscular dystrophy caused by lamin A/C gene mutation
... Figure 2 Increased ERK1/2 activity in skeletal muscle of Lmna H222P/H222P ...phosphorylated ERK1/2 (p-ERK1/2) and total ERK1/2 in protein extracts ...
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<p>Bisperoxovanadium protects against spinal cord injury by regulating autophagy via activation of ERK1/2 signaling</p>
... of p-ERK1/2 in the spines of SCI rats treated with bpV(pic) and ASC combined with bpV(pic) (Figure 4B and ...through ERK1/2 ...of ERK1/2 signaling, SCH772984 (20 ...of ...
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Original Article ERK1/2 phosphorylation in the hippocampus is associated with abnormal anxiety-related and social behaviors in Fmr1 knockout mice
... anti- ERK1/2 (1:10,000 dilution, Cell Signaling Technology, Beverly, MA, USA) or rabbit anti- P-ERK1/2 (1:10,000 dilution, Cell Signaling Technology, Beverly, MA, USA) and mouse anti- ...
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Electroacupuncture mediates extracellular signal regulated kinase 1/2 pathways in the spinal cord of rats with inflammatory pain
... inhibited ERK1/2 activation in ...of ERK1/2 in the analgesic effect of ...lated ERK1/2 signaling pathway, we further investigated EA’s effect on the downstream substance of ...
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<p>NSD2 Promotes Renal Cancer Progression Through Stimulating Akt/Erk Signaling</p>
... Akt, p-Akt (S473), Erk1/2, p-Erk1/2 (Thr202/ Tyr204), Bcl-2, Bax (Cell Signaling Technology, USA) and GAPDH (Keygen Biotech, ...
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Orphan Gpr182 suppresses ERK mediated intestinal proliferation during regeneration and adenoma formation
... IHC. Paraffin and cryogenic sections were rehydrated, permea- bilized, and blocked with 5% normal donkey serum. When required, slides were boiled in citrate buffer for antigen retrieval. Slides were then stained ...
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