p21-Activated kinase 2 (PAK2)
Human Immunodeficiency Virus Type 1 Nef Selectively Associates with a Catalytically Active Subpopulation of p21-Activated Kinase 2 (PAK2) Independently of PAK2 Binding to Nck or β-PIX
... serine-threonine kinase (NAK) as the p21-activated kinase 2 ...transfecting PAK2 cDNA or its mutant derivatives in order to further characterize the Nef-NAK ...of PAK2, we ...
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Nef Associates with p21-Activated Kinase 2 in a p21-GTPase-Dependent Dynamic Activation Complex within Lipid Rafts
... of p21-activated kinase 2 ...a PAK2 activation complex within a detergent-insoluble membrane compartment containing the lipid raft marker ...Nef-associated kinase (NAK) complex ...
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Activation of p21-Activated Kinase 2 by Human Immunodeficiency Virus Type 1 Nef Induces Merlin Phosphorylation
... Three types of vesicular stomatitis virus G protein (VSV-G)- pseudotyped env-negative HIV-1 with an intact SF2 nef, a frameshift mutant nef, or nef F195R were used to infect acti- vated primary human T cells. After ...
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Merlin Phosphorylation by p21-activated Kinase 2 and Effects of Phosphorylation on Merlin Localization
... of PAK2, which can be directly activated by Rac/cdc42, as the merlin kinase functionally connects merlin with the Rac/cdc42 ...effector, PAK2, phosphorylates and, per- haps, inactivates merlin ...
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A Hydrophobic Binding Surface on the Human Immunodeficiency Virus Type 1 Nef Core Is Critical for Association with p21-Activated Kinase 2
... with Pak2, we performed mutational analysis of three primary HIV-1 Nef clones that exhibited similar capacities for downregulation of CD4 and MHC-I but variable abilities to associate with activated ...with ...
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Reconstitution and Molecular Analysis of an Active Human Immunodeficiency Virus Type 1 Nef/p21-Activated Kinase 2 Complex
... portion of the cell-free translation reaction mixture was used to verify protein expression by Western blotting using either an anti-HIV-1 Nef antibody (subtype B) or an anti-SIV (mac239) Nef antibody as appropriate. Nef ...
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Activation of P21-activated protein kinase 2 is an independent prognostic predictor for patients with gastric cancer
... of PAK2 and pSer20PAK2 proteins were significantly higher in gastric cancer than in normal gastric ...between PAK2, pSer20PAK2 expression and aggressive clinicopathological ...of PAK2 and pSer20PAK2 ...
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p21-activated kinase 1 (PAK1) expression correlates with prognosis in solid tumors: A systematic review and meta- analysis
... The results of our meta-analysis suggest that higher tumoral PAK1 expression is associated with an unfavorable prognosis and is predictive factor associated with OS and DSS in patients with solid tumors. PAK1 is an ...
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Inca: a novel p21 activated kinase associated protein required for cranial neural crest development
... restricted to early ectoderm and notochord during gastrulation, as well as later in the premigratory and migrating cranial NC (Fig. 2I- N). Expression persists in the pharyngeal arches, ventral forebrain, pituitary and ...
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GTPase-Activating Proteins for Cdc42
... The Rho-type GTPase, Cdc42, has been implicated in a variety of functions in the yeast life cycle, including septin organization for cytokinesis, pheromone response, and haploid invasive growth. A group of proteins ...
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Down-regulation of p21-activated serine/threonine kinase 1 is involved in loss of mesencephalic dopamine neurons
... expressing wild-type PAK1 (WT), constitutively active PAK1 mutant (PAK1-CA, PAK1 T423E ), dominant- negative PAK1 mutant (PAK1-DN, PAK1 H83,86L, K299R ) [19, 20], or GFP using a neuroblastoma cell line, SH- SY5Y cell ...
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Human immunodeficiency virus type 1 Nef associates with a member of the p21-activated kinase family.
... lane 2) as well as polyclonal anti-Nef antisera (not ...lane 2), and were present at levels, compared to the control, of approxi- mately 3, 3, and 8%, respectively, of precipitated Nef by mass, assuming ...
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PakD, a Putative p21-Activated Protein Kinase in Dictyostelium discoideum, Regulates Actin
... lected and boiled for 3 min in SDS-PAGE sample buffer. Proteins were separated by SDS-PAGE and were transferred to Hybond-P membranes (Amersham Bioscience, Piscataway, NJ) by following the manufacturer’s protocol. The ...
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PubMedCentral-PMC5198172.pdf
... a kinase network ...PAK, p21- activated kinases; PKD, protein kinase D; GRK, G-protein coupled receptor kinase; DMPK, dystrophia myotonica-protein kinase; CK, casein ...
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p21-activated kinase 1: PAK’ed with potential
... figure 2: PAK1 regulates actin cytoskeleton morphology and proliferation of squamous nsclc cells. PAK1 is required for hepatocyte growth factor (HGF)-induced actin dynamics and proliferation of squamous NSCLC ...
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p21 activated kinase signaling in breast cancer
... interaction with 14-3-3 tau. Pak1 activation, and resulting inactivation of Bad, is also sufficient to antagonize p38 MAPK as well as its upstream activator MKK6-induced cell death [46]. In addition, Pak1 utilizes its ...
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Original Article p21-activated kinase 4 promotes proliferation, migration, and invasion of human gastric cancer cells
... protein concentrations were determined using a bicinchoninic acid protein assay (Pierce, Rockford, USA). Next, cell lysates (50 μg pro- tein/lane) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel ...
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Pathogenic Escherichia coli Hijacks GTPase Activated p21 Activated Kinase for Actin Pedestal Formation
... EspG promotes pedestal formation and strong bacterial attachment. To estab- lish the contribution of EspG to pedestal formation, Hap1 cells were infected with either wild-type (WT) EPEC or an espG1 espG2 double-knockout ...
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MEKK1, JNK, and SMAD3 mediate CXCL12-stimulated connective tissue growth factor expression in human lung fibroblasts
... receptor-like kinase 5; AP-1: Activator protein-1; BM: Bone marrow; ChIP: Chromatin immunoprecipitation; CTGF: Connective tissue growth factor; CXCR4: CXC receptor 4; ECM: Extracellular matrix; ERK: Extracellular ...
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Regulation of the mitogen activated protein kinase p44 ERK activity during anoxia/recovery in rainbow trout hypodermal fibroblasts
... MAP kinase kinase kinase (MAP3K) upstream of a MAPK kinase kinase (MAP2K), which in turn activates the MAP kinase (Kyriakis and Avruch, ...MAPK/ERK kinase (MEK)1/2 ...
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