Packaging signal
Cre Levels Limit Packaging Signal Excision Efficiency in the Cre/loxP Helper-Dependent Adenoviral Vector System
... increased packaging signal excision ...First, packaging signal exchange, evidence of the reverse Cre reaction, was not observed between two marked helper viruses following coinfection of ...
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Intracellular Cre-Mediated Deletion of the Unique Packaging Signal Carried by a Herpes Simplex Virus Type 1 Recombinant and Its Relationship to the Cleavage-Packaging Process
... to-helper particles obtained during three successive passages was plotted. As shown in Fig. 1D, the fraction of vector parti- cles serially passaged on TE-671 cells presented a classical up-and-down growth curve of ...
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Secondary Structure Analysis of a Minimal Avian Leukosis-Sarcoma Virus Packaging Signal
... in packaging (3, ...for packaging in the heterologous system, it appears to play an important role in some step of the viral life ...cient packaging of ⌿ would provide further evidence that ...
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In Vivo Selection of Rous Sarcoma Virus Mutants with Randomized Sequences in the Packaging Signal
... Retrovirus genomes contain a sequence at the 5 * end which directs their packaging into virions. In Rous sarcoma virus, previous studies have identified important segments of the packaging signal, C ...
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Encapsidation of turnip crinkle virus is defined by a specific packaging signal and RNA size.
... The results presented here also raise questions about the effect of RNA size on the TCV assembly process. The model of Sorger et al. (32) proposes that assembly would initiate at one site (the OAS) and proceed until the ...
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The Genome-Packaging Signal of the Influenza A Virus Genome Comprises a Genome Incorporation Signal and a Genome-Bundling Signal
... to signal the incorporation of that ...the packaging efficiency of the recom- binant NP vRNA itself, possibly by direct or indirect interactions with the packaging signal of the recombinant NP ...
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Functional Analysis of the Core Human Immunodeficiency Virus Type 1 Packaging Signal in a Permissive Cell Line
... initiation signal (7, 14, 16, 31–34, 40, 46, 49–51, ...core packaging signal region in encapsidation, in permissive cells, by introduction of disrup- tive, compensatory, and deletion ...RNA ...
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The Duck Hepatitis B Virus Polymerase Is Activated by Its RNA Packaging Signal, ɛ
... The hepatitis B viruses (hepadnaviruses) are small DNA- containing viruses that replicate by reverse transcription (re- viewed in reference 3). The interaction between the hepadna- virus polymerase and a stem-loop (ε) at ...
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Effect of the Murine Leukemia Virus Extended Packaging Signal on the Rates and Locations of Retroviral Recombination
... the packaging signal to the 3⬘ UTR could result in packaging of spliced RNA ...the packaging of spliced RNA (21), this could result in a potential bias in the analysis of the locations of the ...
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Importance of Basic Residues in Binding of Rous Sarcoma Virus Nucleocapsid to the RNA Packaging Signal
... or packaging, although the two Cys-His boxes are likely to be required for efficient viral assembly and ...the packaging signal ( ⌿ )-containing ...
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Cooperation of an RNA Packaging Signal and a Viral Envelope Protein in Coronavirus RNA Packaging
... the packaging signal might induce a specific conforma- tional change in N protein that could serve as a nucleation event for the cooperative binding of N protein to the rest of the RNA, thereby generating ...
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Conservation of a Packaging Signal and the Viral Genome RNA Packaging Mechanism in Alphavirus Evolution
... the packaging signal, and more than one stem-loop is required for efficient genomic RNA ...genome packaging, in the case of VEEV (and probably other alphaviruses), the term “minimum PS” is likely to ...
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Identification and characterization of a coronavirus packaging signal.
... This conclusion was supported by the following observations: first, this 61-nucleotide-long sequence was included within two previously mapped putative packaging signal regions, 1,480 nu[r] ...
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RNA secondary structure and binding sites for gag gene products in the 5' packaging signal of human immunodeficiency virus type 1.
... The selective encapsidation of retroviral RNA requires sequences in the Gag protein, as well as a cis-acting RNA packaging signal ( C site) near the 5 * end of the genomic transcript. Gag protein of human ...
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Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region.
... To determine the mechanism underlying the large difference in virus titers produced by PA317 cells containing gag+ vectors in comparison with PA317 cells containing gag- vectors, we firs[r] ...
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Functional Analysis of the Murine Coronavirus Genomic RNA Packaging Signal
... selective packaging of nonviral RNA containing the PS, and this packaging occurred in the absence of N protein ...how packaging selectivity is determined in coronavi- ruses that fall outside ...
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Genetic analysis of the filamentous bacteriophage packaging signal and of the proteins that interact with it.
... 9, who showed that plasmids that contain this hairpin and the phage origin of DNA replication produce and efficiently encapsidate single-stranded plasmid DNA when phage helper functions [r] ...
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Nucleotide sequence and structural features of a novel US-a junction present in a defective herpes simplex virus genome.
... RESULTS The present study was undertaken to determine the nucleotide sequence of a functional cleavage-packaging signal within defective HSV-1 DNA and to define the structural organizati[r] ...
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Rauschhuber, Christina (2011): Analysis of Adenovirus-Host Interactions to Improve Recombinant Adenoviral Vectors for Gene Therapy. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... The most advanced generation of adenoviral vectors, the so-called high-capacity adenoviral vectors (HC-AdV), consists of the two adenoviral ITRs and the packaging signal, but lacking a[r] ...
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Defective herpes simplex virus type 1 vectors harboring gag, pol, and env genes can be used to rescue defective retrovirus vectors.
... retroviral packaging transcription unit was constructed in which the Moloney murine leukemia virus (MoMLV) gag-pol and env genes are expressed under the control of herpesvirus regulatory ...retrovirus ...
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