patch clamp
Primary Cultures of Muscle from Embryonic Locusts (Locusta Migratoria, Schistocerca Gregaria): Developmental, Electrophysiological and Patch Clamp Studies
... This paper describes the methods employed to establish primary cultures of locust muscle tissue and contains a preliminary account of patch-clamp recordings, using giga-ohm seals, from s[r] ...
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Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
... We also performed an ATP fluorescence detection experiment to further confirm the feasibility of our system. We changed the patch clamp system to a fluorescence detection system. When ATP samples were ...
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Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings
... cell-attached patch under simultaneous whole-cell v-c conditions with a voltage command waveform composed of the addition of the voltage command step and the intracellular voltage deviation recorded under ...
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Patch clamp evidence for calcium channels in apical membranes of rabbit kidney connecting tubules
... tubules (CNTs). Single channel Ca currents were recorded with Ba as the charge carrier. In the cell-attached mode, 8-Br-cAMP increased the open-state probability (Po) to 0.6%. In excised, inside-out patches, Po was low ...
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Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
... for patch-clamp analysis In this study, HEK 293-hERG cells (Merck Millipore, Billerica, MA, USA), which are HEK 293 cells stably ex- pressing hERG, and CHO-hERG cells (Merck Millipore), which are CHO cells ...
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GABA-mediated inhibitory postsynaptic currents recorded in rat hippocampal slices using intracellular and patch clamp recording techniques
... voltage clamp technique and a standard slice ...using patch clamp techniques in brain slices allowed the study of hippocampal IPSCs to be contin ued with a much higher ...the patch ...
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Automatic tracking of cells for video microscopy in patch clamp experiments
... Optogenetic activation of neurons is a technique that is becoming customary in almost every neuroscience lab [10]. In general, optogenetic constructs carry the coding for flu- orescent proteins to indicate cells that are ...
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A patch-clamp study of native and recombinant glutamate receptors
... the patch shown in Fig ...other patch gave an open time distribution with two exponential components: ...The patch illustrated in Fig ...during patch-clamp ...
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Chao, Yu-Kai (2019): Functional characterization of intracellular ion channels with the endolysosomal patch-clamp technique. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... We show here that we can selectively patch clamp subpop- ulations of endolysosomal organelles by using distinct small molecules for specific vesicle enlargement. Thus, we can demonstrate where exactly ion ...
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A patch clamp study of acetylcholine activated ion channels in Ascaris suum muscle
... Desensitization at higher agonist concentrations Experiments at 25 ^mol P 1 acetylcholine N=5 produced a different pattern of channel opening to that observed at lower concentrations; si[r] ...
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The vertebrate neuronal gamma aminobutyric acid (GABAa) receptor and its modulation: a patch clamp study
... Filled patch pipettes were firmly secured onto the pipette holder containing the Ag/AgCl wire which In turn was connected to the amplifier headstage. Positive or negative pressure could be applied to the pipette ...
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Patch clamp analysis of the effects of the insecticide deltamethrin on insect neurones
... Effects of membrane potential on the relative mean open time A and the relative open time probability B of the deltamethrin-induced single-channel activity from different cell-attached p[r] ...
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Patch clamp Study of the Properties of the Sodium Current in Cockroach Single Isolated Adult Aminergic Neurones
... Kinetics of the STX-sensitive inward current The kinetic properties of the inward sodium current inactivation were studied using the time course of sodium current decay during a single p[r] ...
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The influence of 8-prenylnaringenin on the activity of voltage-gated kv1.3 potassium channels in human jurkat t cells
... Abstract: Using the whole-cell patch-clamp technique, we investigated the influence of 8-prenylnaringenin on the activity of the voltage-gated Kv1.3 potassium channels in the human leukemic T lymphocyte ...
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A missense mutation in the Kv1 1 voltage gated potassium channel–encoding gene KCNA1 is linked to human autosomal dominant hypomagnesemia
... tal conditions (Figure 3E). Subsequently, the current clamp mode of the patch clamp technique was used to measure the membrane potential of HEK293 cells expressing wild-type Kv1.1 or Kv1.1 N255D. A ...
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Modulation of the intrinsic neuronal excitability by multifunctional liposomes tailored for the treatment of Alzheimer's disease
... To unravel the reason behind the coexistence of popula- tions of cells firing at different frequencies, we observed the distribution of the fluorescent nanoliposomes on the neural cells cultures under similar conditions ...
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Electrophysiological Study of Freshly Isolated Articular Chondrocytes Vs. Cryopreserved Chondrocytes
... study patch clamp technique was used to study the Potassium channel currents in chondrocytes before and after ...with patch clamp to make a decisive statement about phenotype maintenance in ...
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Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles.
... The development of safe technologies to genetically modify neurons is of high interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily ...
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Reduced presynaptic vesicle stores mediate cellular and network plasticity defects in an early-stage mouse model of Alzheimer’s disease
... Evoked responses were analyzed offline using Clampfit 10 and Graphpad Prism 6 software. For PTP recordings, data were recorded 0–2 min post-tetanus, and for e-LTP, 15–20 min post-tetanus. Evoked EPSPs were averaged and ...
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Developmental changes in spinal neuronal properties, motor network configuration and neuromodulation at free swimming stages of Xenopus tadpoles
... NEW & NOTEWORTHY A novel in vitro brainstem/spinal cord preparation is described that enables whole cell patch-clamp recordings from spinal neurons in pro-metamorphic Xenopus tadpoles.. [r] ...
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