PC12 cell
Graphene oxide and reduced graphene oxide induced neural pheochromocytoma-derived PC12 cell lines apoptosis and cell cycle alterations via the ERK signaling pathways
... examined PC12 cell viability using the CCK-8 assay, which revealed that GO and rGO induce a significant decrease in cell viability in both time- and dose- dependent manner, with GO inducing more ...
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Intermittent hypoxia-induced protein phosphatase 2A activation reduces PC12 cell proliferation and differentiation
... IH-exposed PC12 cells (p < ...IH-exposed PC12 cells’ death, an Annexin V assay was used to deter- mine the percentages of viable cells (M1), apoptotic frac- tions (M2) and necrotic fractions ...
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Combination of Polymer Technology and Carbon Nanotube Array for the Development of an Effective Drug Delivery System at Cellular Level
... on PC12 cell line have been performed in order to assess the efficiency and functionality of the entrapped agent (neural growth factor, ...NGF). PC12 cells differenti- ation following incubation on ...
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Original Article Tempol protect against hypoxia induced oxidative stress in PC12 cells
... that cell has an innate antioxidant system including SOD, CAT and GSH-Px and GSH which inhibit the formation of ROS and keep ROS at a basal non-toxic level ...in PC12 cell under hypoxia was ...
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Original Article GATA3 modulates neuronal survival through regulating TRPM2 in Parkinson’s disease
... lated PC12 cells proliferation and survival under 6-OHDA treatment and significantly down-regulated TRPM2 ...6-OHDA-induced PC12 cell pro- liferation and ...
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Identification of cis-acting sequences in the promoter of the herpes simplex virus type 1 latency-associated transcripts required for activation by nerve growth factor and sodium butyrate in PC12 cells.
... of PC12 cells with NGF or ...with PC12 cell nuclear extracts ...NaB-treated cell extracts, B1/B2 formed as a doublet similar to the complex that formed with untreated cell extracts; ...
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-induced apoptosis in PC12 cells by suppressing the expression of endoplasmic reticulum stress-related proteins
... [20]. Moreover, many apoptotic insults, includ- ing Aβ, cause neuritic degeneration. Aβ can induce neuronal apoptosis by a mechanism which involves the c-Jun N-terminal kinase pathway and the induction of Fas ligand ...
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Akt regulates the expression of MafK, synaptotagmin I, and syntenin-1, which play roles in neuronal function
... stable PC12 cell lines that con- stitutively overexpress WT-Akt1 or DN-Akt1 ...as PC12 (WT-Akt) or PC12 (DN- Akt). These two PC12 sublines have different neurite out- growth phenotypes ...
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Original Article Differential gene expression profile analysis in corticosterone-treated PC12 cells
... live PC12 cell num- bers, we identified highly enriched differentially expressed genes promoting apoptosis and inhibiting cell division, consistent with known functions and mechanisms of ...
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Reversible optogenetic control of kinase activity during differentiation and embryonic development
... a PC12 cell differentiation assay. In the PC12 cell differentiation assay, the differentiation ratio yielded by co- transfection of CIBN-GFP-CaaX and CRY2-mCherry-Raf1 and subsequent blue ...
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Cocaine induces cell death and activates the transcription nuclear factor kappa-b in pc12 cells
... cocaine-induced cell toxicity as assessed by LDH and MTT ...in PC12 cell viability induced by cocaine was more pronounced in the presence of the NF-κB inhibitors (Figure ...in cell death ...
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Agonists of CB1 and NMDA receptors decrease the toxic effect of organophosphorus compound paraoxon on PC12 cells
... decreases cell proliferation and increases cell death of human GBM (glioblastoma multiforme) cells ...pheochromocytoma, PC12 cell line is a useful model for neuroprotection studies [14, ...
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Inhibition of ROS-mediated activation Src-MAPK/AKT signaling by orientin alleviates H<sub>2</sub>O<sub>2</sub>-induced apoptosis in PC12 cells
... in PC12 cells but significantly decreased H 2 O 2 -induced reduction in PC12 cell viability, cell apoptosis rates, and nuclear ...in PC12 cells were activated in a time-dependent ...
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NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model
... the PC12 cell sample was collected for western blot ...promotes cell survival [30]; thus, we determined the effect of the lentiviruses on Akt activa- ...induces cell death, compared to NT3, ...
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Differential effects of nerve growth factor and dexamethasone on herpes simplex virus type 1 oriL- and oriS-dependent DNA replication in PC12 cells.
... performed. PC12 cell nuclear extracts were incu- bated with the probe containing oriL GRE and a 100-fold excess of unlabeled competitor probe (lanes 4 to ...in PC12 cell nuclear extracts is ...
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Zika Virus Envelope Protein induces G2/M Cell Cycle Arrest and Apoptosis via an Intrinsic Cell Death Signaling Pathway in Neuroendocrine PC12 Cells
... Figure 5 Effects of caspase inhibitors on ZIKV Env-mediated apoptosis in PC12 cells. (A) Cells were transduced with LV-ZIKV-prM-Env, LV-ZIKV-M-Env, LV-ZIKV-Env or LV-eGFP (100 MOI) for 6 h followed by treatment ...
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Erythropoietin prevents PC12 cells from beta amyloid induced apoptosis via PI3K⁄Akt pathway
... Biotechnology, Rockford, IL). Equal amounts of the pro- tein (20 μg) were separated in an 8-10% SDS-polyacryla- mide gel; the resolved proteins were electrotransferred onto PVDF or nitrocellulose membranes (Bio-Rad, Her- ...
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Original Article Neuroprotective effect of hyperoside on human PC12 cells against the oxidative damage
... in PC12 cells with H 2 O 2 ...in PC12 cells exposed to H 2 O 2 , while HY (2, 10 and 50 μg/ml) dose- dependently reduced caspase 3 mRNA expres- sion by ...
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Involvement of Programmed Cell Death in Neurotoxicity of Metallic Nanoparticles: Recent Advances and Future Perspectives
... The widespread application of metallic nanoparticles (NPs) or NP-based products has increased the risk of exposure to NPs in humans. The brain is an important organ that is more susceptible to exogenous stimuli. ...
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Novel autocrine feedback control of catecholamine release A discrete chromogranin a fragment is a noncompetitive nicotinic cholinergic antagonist
... dium–washed PC12 cells; after an 18-h (overnight) incubation at 37 8 C, the medium was collected, and 5 vols of acetone (at 2 20 8 C) were added to precipitate proteins and peptides while small mole- cules (such ...
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