PCR and sequencing primers
Polyadenylated Sequencing Primers Enable Complete Readability of PCR Amplicons Analyzed by Dideoxynucleotide Sequencing
... using sequencing primers with 40–80 bp neutral polynucleotide tails ...tailed primers, however, could in some cases form secondary structures between the tail and the primer 3′ end resulting in a ...
5
A database of PCR primers for the chloroplast genomes of higher plants
... utilized PCR-amplified chloroplast gene sequences for establishing and verifying ...of primers recommended for this purpose have also expanded in size ...
7
PCR & DNA Sequencing. PCR= Polymerase Chain Reaction. PCR applications
... of PCR reaction: • As a rule of thumb, use an annealing temperature (Ta) about 5 o C below the lowest Tm of the pair of primers to be used if a good yield of product is ...
11
Detection of Mycobacterium ulcerans by real-time PCR with improved primers
... results, PCR sensitivity was more signifi- cantly improved by new primers for three Japanese iso- ...conventional primers than other ...new primers improved the sensitivity of M. ulcerans ...
6
Design of an improved set of oligonucleotide primers for genotyping MeCP2tm1.1BirdKO mice by PCR
... and sequencing the PCR fragment amplified on the KO allele, we tested several sets of primers that were designed to yield PCR fragments of different sizes on the KO and WT ...three ...
6
Universal Primers for Detection and Sequencing of Hepatitis B Virus Genomes across Genotypes A to G
... genome primers had a higher chance of nonspecific amplification of smaller frag- ments than did the overlapping primer ...some PCR reagents in the first round of amplification, the use of the later primer ...
5
Serotype Identification of Group B Streptococci by PCR and Sequencing
... by PCR. We designed MS-specific primers for MSs Ia, Ib, III, IV, V, and VI and confirmed their specificities, initially using two serotype reference panels for ...MS-specific PCR alone was used to ...
11
Comprehensive Detection and Serotyping of Human Adenoviruses by PCR and Sequencing
... Sequencing and phylogenetic analysis. Figure 1 displays a phylogenetic tree built from an alignment of the amino acid sequences of the amplicons of each serotype prototype. Figure 2 displays a phylogenetic tree ...
7
PrimerSeq: Design and visualization of RT-PCR primers for alternative splicing using RNA-seq data.
... RNA sequencing (RNA-seq) has become a powerful technology for transcriptome-wide analysis of AS, but RT-PCR still remains the gold-standard approach for quantifying and validating exon splicing ...
6
Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR
... 3 Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary # author for correspondence: [email protected] Summary: Several grapevine pathogens are ...
10
Virus detection and identification using random multiplex (RT) PCR with 3' locked random primers
... Our long-term goal is to provide emergency room and pri- mary care physicians with the ability to universally detect viruses in human plasma. The rationale is, in part, that if an emerging or recombined pathogenic virus ...
11
Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR
... 3 Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary # author for correspondence: [email protected] Summary: Several grapevine pathogens are ...
10
PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data
... RNA sequencing (RNA-seq) has become a powerful technology for transcriptome-wide analysis of AS, but RT-PCR still remains the gold-standard approach for quantifying and validating exon splicing ...
5
Automated degenerate PCR primer design for high throughput sequencing improves efficiency of viral sequencing
... degenerate PCR primer design system that plays a key role in the ...population. PCR primer pairs are then selected computationally to produce a minimal amplicon set capable of tiling across the full length ...
9
Sequetyping: Serotyping Streptococcus pneumoniae by a Single PCR Sequencing Strategy
... Differences between the predicted in silico analysis data and the experimental sequetyping results were observed. Serotypes 27 and 29, which were not predicted to produce an amplicon, were in fact amplified and ...
9
Identification of Novel HelicobacterSpecies in Pig Stomachs by PCR and Partial Sequencing
... a PCR with Helicobacter genus-specific ...95.0% PCR-positive results, ...of PCR-positive cases relative to severity of the ...and PCR-restriction fragment length polymorphism (RFLP) patterns ...
5
Comparative Study of Direct Bisulfite Sequencing PCR and Methylation Specific PCR to Detect Methylation Pattern of DNA
... bisulfite sequencing, MSP, combined bisulfite wrong ...etc. sequencing is a more confident method mainly known as Bisulfite direct sequencing is the most a quantitative method representing precise ...
5
Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... the PCR assays used for detecting mycobacteria involve spe- cies-specific primers targeting the 16S rRNA, hsp65, 32-kDa protein genes, or the internal transcribed spacer (ITS) and detect only a single or a ...
7
PCR primers Universal primers for PCR am-
... in PCR amplification and transcrip- tion of two PVY isolates in a mixed ...strain-specific primers for the separate amplification of each target sequence in a mixed virus ...
7
Development of a Universal Intimin Antiserum and PCR Primers
... the PCR primers synthesized to generate Int 388–667 gave a specific PCR product with as the template eae-positive strains belonging to different EPEC and EHEC serogroups isolated from a variety of ...
6