PCR Assays
Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... Real-time PCR is a powerful tool for detection of human patho- gens because of the high sensitivity and throughput ...real-time PCR allows simultaneous detection of multiple organisms in a single reaction, ...
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The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays
... Spne-PCR, described here, and psaA-PCR [17] were the only two out of 5 PCR assays tested, which did not mis- identify S. pseudopneumoniae as S. pneumoniae. The approach using representative ...
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Comparison of Quantitative and Qualitative PCR Assays for Cytomegalovirus DNA in Plasma
... standardized assays that detect and quantify CMV DNA in clinical ...Such assays would reduce the variation in viral load results among different in- house-developed CMV PCR ...in-house assays ...
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Real Time PCR Assays for Detection of Bocavirus in Human Specimens
... time PCR assays that benefit from a closed tube system that minimizes the potential for amplicon contamination and by requiring two independent assay targets (NS1 and NP-1) to be positive in the same sample ...
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Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
... real-time PCR assays described here were significantly more sensitive than FA for the detection of respiratory viruses in clinical specimens from children, detecting 40% more pos- itive specimens and ...
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Duplex Reverse Transcription PCR Followed by Nested PCR Assays for Detection and Identification of Brazilian Alphaviruses and Flaviviruses
... (RT-PCR) assays using species-specific primers have been de- scribed for certain alphaviruses (3, 19, 20, 25, 28, 33, 38, 39) and certain flaviviruses (7, 8, 9, 12, 13, 16, 21, 23, 27, ...
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Performance and Cost Evaluation of One Commercial and Six In House Conventional and Real Time Reverse Transcription PCR Assays for Detection of Severe Acute Respiratory Syndrome Coronavirus
... seven assays; 46 were negative and 17 were positive, with only 5 discordant ...more assays, there were a total of 18 positives and 50 ...seven assays were similar, with sensitivities ranging from ...
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Comparison of Commercial Real Time Reverse Transcription PCR Assays for Reliable, Early, and Rapid Detection of Heterologous Strains of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected or Noninfected Boars by Use of Different Sample Types
... the PCR assays ...among assays using field samples from historically PRRSV ELISA-negative herds, the AD assay resulted in ...AD assays also generated false-positive results in experimental ...
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Comparison of PCR Assays for Diagnosis of Cutaneous Leishmaniasis
... ITS1 PCR was also shown to be more sensitive ...this PCR assay was slightly less sensitive than the kDNA PCR, missing the six C-Pos sam- ples ...the PCR assays’ detection limits explain ...
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Development of Real Time PCR Assays for Genotyping of Chlamydia trachomatis
... real-time PCR. Real-time PCR was performed using a Rotorgene 3000 apparatus (Corbett Robotics, Australia) essentially as described previously but with a few modifications ...1A). PCR was performed in ...
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Differentiation of Leptospira interrogans isolates by IS1500 hybridization and PCR assays
... Using the inverse complement of primers P1 and M16 (iP1 and iM16, respectively), a PCR-based typing assay was devel- oped. These primers direct amplification outward from IS1500 to sequences that flank the ...
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Predicting the sensitivity and specificity of published real-time PCR assays
... A freely available real time PCR analysis tool called TaqSim [4] was used to find public sequences that would match the primer/probe assay in question. TaqSim uses BLAST searches to find sequences that match both ...
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Development of real-time PCR assays for the quantitative detection of herpesviruses
... Kaposi’s sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV- 8) and Epstein-Barr virus (EBV) or human herpesvirus 4 (HHV-4) are both members of the viral subfamily of gamma herpesviruses. KSHV is an ...
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Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... Group A consisted of 104 plasma samples obtained from patients at the 302 Military Hospital of China and group B consisted of 59 plasma samples obtained from patients at Beijing ChaoYang Hospital between July 2016 and ...
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Development and Validation of a Quantitative PCR Assay for Diagnosis of Scedosporiosis
... conventional PCR assays have been developed to detect Scedosporium DNA (1, 11, 22), but to date no methods based on quantitative PCR methodol- ogies to detect these species have been ...
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Comparison of characteristics of Q beta replicase amplified assay with competitive PCR assay for Chlamydia trachomatis
... Although PCR assays directed against the cryptic ...of PCR assays targeted against either the cryptic plasmid or the 16S rRNA, and in order for the assay performance to be comparable on all ...
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Improved Mg2+ Based Reverse Transcriptase Assay for Detection of Primate Retroviruses
... RNA PCR assays to demonstrate the absence of detectable HIV or SIV in several monovalent lots of oral, poliovirus vaccine ...similar assays and because of its low background ...
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Is the Perceived Association between Chlamydia pneumoniae and Vascular Diseases Biased by Methodology?
... following PCR assays. Assay A1 was a single-step C. pneumoniae PCR targeting the 16S rRNA gene essentially as described by Gaydos et ...pneumoniae PCR targeting the ompA gene, essentially as ...
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Development and Evaluation of Real Time PCR Based Fluorescence Assays for Detection of Chlamydia pneumoniae
... standardized PCR assays are needed. Two real-time PCR assays specific for ...two PCR assays was ...VD4 assays, producing the expected 108- and 125-bp amplification ...
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Real Time Reverse Transcription PCR Assay Panel for Middle East Respiratory Syndrome Coronavirus
... RT-PCR assays, false-posi- tive rRT-PCR results can still occur if practices designed to mini- mize the risk of contamination are not stringently ...rRT-PCR assays targeting different ...
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