PCR DGGE
Pcr dgge based diversity analysis of bacteria and archaea from mud volcanoes on Andaman Islands, India
... Mud volcanoes at Baratang and Kattan started ejecting mud after the 2004 Tsunami incidence. In this study, prokaryotic diversity in the flowing mud was investigated by using PCR-DGGE approach. 88 organisms ...
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Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens
... by PCR using primers PRBA338F (5' CGC CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG GAC TCC TAC GGG AGG CAG CAG 3'; GC-clamp is in boldface) [19] and PRUN518R (5' ATTA CCG CGG CTG CTGG 3') ...final PCR ...
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Applicability of PCR DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion
... the PCR with 16S rDNA targeted universal primers proved to be far more sensitive than conventional culture ...of PCR- based assays is that they target only one or in some stud- ies up to four pathogens ...
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Bacterial Colonization of the Equine Gut; Comparison of Mare and Foal Pairs by PCR DGGE
... of PCR- DGGE and similar ...[29,30]. PCR-DGGE results are not indicative of popula- tion size, and quantitative biases associated with similar methods have been demonstrated ...
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PCR DGGE analysis of earthworm gut bacteria diversity in stress of Escherichia coli O157:H7
... of PCR-DGGE fingerprint- ing and ecology index, we speculated that the appearance of band c lead a short increasing of diversity and abun- dance index in the first ...
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Elucidation of The Microbial Community in Activated Sludge using PCR-DGGE Analysis in Arid and Semi Arid Regions of Rajasthan
... of PCR- amplified 16S ribosomal DNA fragments. Dominant bands from DGGE profiles were excised and subjected to sequencing to identify the dominant ...the DGGE profiles indicated significant diversity ...
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Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis (PCR DGGE) Profile of Bacterial Community from Agricultural Soils in Bauchi, North East Nigeria
... The need to understand the microbial community that channel soil affair is quite imperative. These will pave way in knowing their activities and mechanis- tic role to the sustenance of ecosystem (e.g. energy flow, ...
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PCR DGGE assessment of the bacterial diversity of breast milk in women with lactational infectious mastitis
... hand, DGGE bands corresponding to the rest of the staphylococcal species could not be identified despite the fact that they were isolated from culture ...the DGGE gels that did not cor- respond to any band ...
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Characterization of the Dominant Bacterial Communities Associated with Terrestrial Isopod Species Based on 16S rDNA Analysis by PCR DGGE
... The DGGE profile of the species A. officinalis showed a band which sequences are homologous to that of Mycoplasma symbiotic of arthropods. Indeed, it has been shown that this bacterium is endowed with a tissue ...
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Microbial Community Analysis of the Anaerobic Digestion Process and the Early Stage of Composting of Pig Manure Using PCR-DGGE
... the DGGE fingerprint were selected and identified, and showed that the majority of clones were uncultured bacteria that are observed during animal manure disposal or anaerobic ...
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PCR-DGGE analysis of fungal community in manufacturing process of a traditional Iranian cheese
... The main fungi that were detected in all samples by DGGE included three different species of Candida (C. pararugosa, C. sake, and C. zeylanoides), two species of Kluyveromyces (K. marxianus, K. lactis), C. ra- ...
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TERRESTRIAL GREEN ALGAE DIVERSITY IN OIL PALM PLANTATION AND SECONDARY FOREST USING 18S-rDNA PCR-DGGE APPROACH
... with PCR and DGGE, and analysis of the 18S rDNA gene fragments obtained from DGGE gel images (Figure 1) from 3 different sampling sites yields a total of 100 bands (S1 = 34; S2 = 13; OP= ...positive ...
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PCR-Denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora
... nested PCR strategies. The first nested PCR strategy uses family-specific primers in the first ...nested PCR strategy and the pres- ence of both Gigaspora and Scutellospora species in the mixed ...
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High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... tions may influence the host immune response and, potentially, the results obtained. Furthermore, enteric helicobacters such as H. hepaticus and H. typhlonius have been shown to cause chronic liver and enteric diseases ...
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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... In DGGE, PCR-amplified DNA fragments of the same length but with different base-pair sequences can be ...for PCR-DGGE, unknown and/or uncultivable species can be de- ...tected. ...
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Conversion of furfural residue to biofertilizer usingBacillus subtilisL7 by solid state fermentation method
... A strain characterized as Bacillus subtilis isolated from soil was used to convertfurfural residue (FR) to biofertilizerbysolid-state fermentation (SSF). Medium for the SSF fermentation was composed of 5% glucose, 2.5% ...
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Molecular Alteration Analysis of Human Gut Microbial Composition in Graves' disease Patients
... The PCR-DGGE of 16S rRNA gene by targeting V3 region and Real-time PCR for Lactobacillus, Bifidobacterium, Bacteroides vulgatus and Clostridium leptum, were ...
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Alterations of Bacteroides sp , Neisseria sp , Actinomyces sp , and Streptococcus sp populations in the oropharyngeal microbiome are associated with liver cirrhosis and pneumonia
... a Cluster analysis of DGGE profiles of the oropharyngeal mucosal microbiome; b Multidimensional scaling analysis MDS based on the predominant oropharyngeal bacterial PCR-DGGE profiles.. [r] ...
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Comparison of different DNA-extraction techniques to investigate the bacterial community of marine copepods
... and PCR-DGGE ...the DGGE-banding patterns of intact and ground copepods extracted with phenol–chloroform, clearly suggesting that there are differences between the bacteria populations in- and ...
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Changes in soil microbial diversity and control of Fusarium oxysporum in continuous cropping cucumber greenhouses following biofumigation
... of PCR-amplified 16S rRNA gene fragments has been widely used to analyze the composition and diversity of microbial communities present in natural environments (Omirou et ...the PCR-DGGE method to ...
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