peripheral mononuclear blood cells
Ex Vivo Stimulation and Expansion of both CD4+ and CD8+ T Cells from Peripheral Blood Mononuclear Cells of Human Cytomegalovirus-Seropositive Blood Donors by Using a Soluble Recombinant Chimeric Protein, IE1-pp65
... dendritic cells (for a review, see reference ...dendritic cells have the capacity to deliver exog- enous Ag in the cytosolic pathway, as we recently demon- strated using pp65-positive apoptotic bodies (2), ...
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Clinical significance of stanniocalcin-1 detected in peripheral blood and bone marrow of esophageal squamous cell carcinoma patients
... in mononuclear cells was extracted by Trizol reagent (Invitrogen, Carlsbad, CA, USA) and RNA concentration was measured by spectrophotometer (Bio- Photometer, Eppendorf, Hamburg, ...
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Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).
... overlapping cells (100 cells per coded slide) per culture well, in a blind ...These cells were scored visually, according to tail size, into the following 4 classes: class 0 - no tail; class 1 - tail ...
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Is the clinical use of adult stem cells a realistic possibility for myocardial regeneration?
... involved cells originating from heterogeneous populations of BMSCs, and as such their identity of each type is not easily distin- ...marrow mononuclear cells (BMMNCs), peripheral blood ...
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Bone marrow T cells from the femur are similar to iliac crest derived cells in old age and represent a useful tool for studying the aged immune system
... of peripheral mononuclear cells [5], which makes them unsuitable for studies in which ...immune cells needs to be ...T cells from the iliac crest and the femur ...T cells in the ...
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Bovine lentivirus induces early transient B-cell proliferation in experimentally inoculated cattle and appears to be pantropic.
... either mononuclear cell number increases or detectable enlargement of lymphatic nodules in experimentally infected ...host peripheral blood lymphocyte populations following infection with BIV are ...
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Immunomodulatory mechanisms of lactobacilli
... by peripheral blood mononuclear cells and monocytes in co-culture with the dlt mutant and wild type strain ...epithelial cells and peripheral blood mononuclear ...
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Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
... (PHA induced PBMCs proliferation) did not show any significant differences among the groups. This assay basically evaluate the function of PBMCs to proliferate in response to a mitogen (PHA). Since we have used a ...
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Effect of Contaminating Red Blood Cells on OKT3-Mediated Polyclonal Activation of Peripheral Blood Mononuclear Cells
... studied cells of the hematopoietic system because of their central role in the generation of immune ...ripheral blood, lymphoid tissues, and certain internal ...ripheral blood compartment and ...
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Donor variability in HIV binding to peripheral blood mononuclear cells
... permissive cells does not occur until 10 minutes after virus is added to cells at 37°C [18] and does not take place at 20°C, while binding of HIV can occur at 4°C or higher temperatures ...of cells ...
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MicroRNA 155 as a proinflammatory regulator via SHIP 1 down regulation in acute gouty arthritis
... in peripheral blood mononuclear cells (PBMCs) from healthy controls (HCs), PBMCs and synovial fluid mononuclear cells (SFMCs) of gouty arthritis ...THP-1 cells were ...
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Viability and Functionality of Cryopreserved Peripheral Blood Mononuclear Cells in Pediatric Dengue
... nonviable cells in children with dengue found here is consistent with previous re- ports that used propidium iodide and annexin V ...dead cells were comparable in children with or without antigenemia ...
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Gene expression signatures for autoimmune disease in peripheral blood mononuclear cells
... The relatively new technology of DNA microarrays offers the possibility to probe the human genome for clues to the pathogenesis and treatment of human disease. While early studies using this approach were largely in ...
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Original Article CD38 positive natural killer cells may be involved in progression of inflammation in rheumatoid arthritis patients
... (NK) cells in peripheral blood (PB) and synovial fluid (SF) in the pathogenesis of rheumatoid arthritis ...of peripheral and synovial NK cells were analyzed by flow ...cytometry. ...
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Functional Measurement of Hepatitis C Virus Core Specific CD8+ T Cell Responses in the Livers or Peripheral Blood of Patients by Using Autologous Peripheral Blood Mononuclear Cells as Targets or Stimulators
... The development of vectors for transducing genes into pri- mary PBMCs, e.g., murine amphotropic retroviral vectors (10, 15, 27, 36), adeno-associated viral vectors (29), and nonviral vectors (20, 39), has received ...
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The multi-differentiation potential of peripheral blood mononuclear cells
... A novel population of PBMCs with fi broblast proper- ties, termed ‘fi brocytes’, was characterized by its distinctive CD45+/CD34+/CD14- phenotype [8]. Fibro- cytes from human male PBMCs rapidly and specifi cally entered ...
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Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia — A 31P MRS in vitro study
... the blood and the acetylohydrolase (AHA) levels in the serum of 79 patients with lymphoid (NHL, HD, LLC, MM) and non-lymphoid (AML, RAEB, MLC, PRV) hematological ...Lower blood PAF levels were found in all ...
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A Preponderance of CCR5+CXCR4+ Mononuclear Cells Enhances Gastrointestinal Mucosal Susceptibility to Human Immunodeficiency Virus Type 1 Infection
... the blood and mucosal compartments might also determine infec- ...and peripheral blood samples of HIV-1-seropositive pa- ...to peripheral blood-derived virus, we would expect that the ...
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Ethyleneglycol-Bis-(β-Aminoethylether)Tetraacetate as a Blood Anticoagulant: Preservation of Antigen-Presenting Cell Function and Antigen-Specific Proliferative Response of Peripheral Blood Mononuclear Cells from Stored Blood
... in peripheral blood often serves as a useful marker of disease progression and prognosis, especially in infections such as HIV type ...when blood samples are to be used for techniques such as ...
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High prevalence of Borna disease virus infection in healthy sheep in Japan.
... in peripheral blood mononuclear cells (PBMCs) from healthy sheep bred on the same ...the blood, as observed in lambs, is usually reduced in adulthood by raising immune responses to ...
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