Permeabilized cell
Fractal morphology of Beta vulgaris L. cell suspension culture permeabilized with Triton X-100®
... were permeabilized with Triton X-100® (Sigma) according to the method developed by Trejo-Tapia et ...(2007). Cell suspension cultures of 6 day old (expo- nential grow cells) were inoculated in 50 ml ...
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Osmotic and thermal effects on in situ ATPase activity in permeabilized gill epithelial cells of the fish Gillichthys mirabilis
... these cell suspensions with alamethicin prior to their addition to ATPase reagent was necessary to allow channel formation by the ...whole permeabilized cells, in homogenates and in permeabilized ...
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Regulation of ion channels by inositol trisphosphate and diacylglycerol
... In these permeabilized cells, the release of calcium from the endoplasmic reticulum by Ins 1,4,5P3 occurs fast enough for it to be able to stimulate various physiological processes such [r] ...
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Comparative molecular field analysis of benzothiazepine derivatives: Mitochondrial sodium calcium exchange inhibitors as antidiabetic agents
... Experimental values are the biological activity values IC50, in μM, inhibition of mNCE mediated Na+/Ca2+ translocation in mitochondria in permeabilized cells monitored, using Ca2+ sensin[r] ...
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Selective Membrane Permeabilization by the Rotavirus VP5* Protein Is Abrogated by Mutations in an Internal Hydrophobic Domain
... not permeabilized by IPTG induc- tion of the recombinant VP8* protein or the pET-6HIS vector ...474 permeabilized BL21(DE3) cells upon IPTG induction. VP4 also permeabilized cells at low but ...
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Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor mu 2 kinase
... (c) Permeabilized cell membranes incu- bated as indicated were reisolated and subjected to immunoblotting with antibodies against the brain-specific insert of -adaptin or with anti-phospho-Thr156 2 ...in ...
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Topography of the Human Papillomavirus Minor Capsid Protein L2 during Vesicular Trafficking of Infectious Entry
... and permeabilized with a low concentration of ...are permeabilized under our conditions as the com- position of these vesicles may be similar to that of the cholesterol- rich plasma ...
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Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells
... and permeabilized rat cardiomyocytes in solution ...the permeabilized (‘skinned’) muscle fibers ...the permeabilized fiber technique needs only a few milligrams of muscle tissue, which is crucial, ...
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Experimental substantiation of permeabilized hepatocytes model for investigation of mitochondria in situ respiration
... It is obvious that the optimal ratio of the digi- tonin concentration to the number of cells in suspen- sion will be different for various tissues. For ade- quate selection of the ratio it is necessary not only to assess ...
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An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria
... A quantitative assay was performed to assess the amount of the intracellular proteins lost during the B-PER treat- ment in order to probe the degree of cell lysis. To this end, a strain of Synechocystis 6803 ...
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Structure and Assembly of Intracellular Mature Vaccinia Virus: Thin-Section Analyses
... Figure 1B shows a cryosection of a group of IMV particles in the perinuclear region of an infected cell. Based on its overall density, and by comparison with results from an earlier, quan- titative study (10), ...
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Vaccinia Virus H2 Protein Is an Essential Component of a Complex Involved in Virus Entry and Cell-Cell Fusion
... unidentified, cell surface receptors ...certain cell signaling pathways (6, 18, ...the cell surface (8, 10). Low pH also triggers cell-cell fusion after large numbers of purified IMV ...
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NmPin from the marine thaumarchaeote Nitrosopumilus maritimus is an active membrane associated prolyl isomerase
... of the archaea [49], N. maritimus very likely pos- sesses a protein-based surface layer (S-layer) on the outer side of the membrane, which can be visualized with trans- mission electron microscopy (TEM) as an additional ...
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Activation of NADPH oxidase-related proton and electron currents in human eosinophils by arachidonic acid
... subsequent application of AA. DPI is believed to inhibit NADPH oxidase by an irreversible chemical reaction (O’Donnell et al. 1994). However, DPI and the related compound iodinium biphenyl appear to interact with NADPH ...
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Toxoplasma gondii Parasitophorous Vacuole Membrane-Associated Dense Granule Proteins Regulate Maturation of the Cyst Wall
... ABSTRACT After differentiation is triggered, the tachyzoite-stage Toxoplasma gondii parasitophorous vacuole membrane (PVM) has been hypothesized to transition into the cyst membrane that surrounds the cyst wall and ...
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Flavivirus Replication Complex Assembly Revealed by DNAJC14 Functional Mapping
... X-100-treated cell lysates to evaluate the flotation behavior of GFP fused to the DNAJC14 pTM region (GFP-pTM) and the noninhibitory mutant construct lacking TMs (NT5CT1) in the absence or presence of YFV infec- ...
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Effect of levosimendan on the contractility of muscle fibers from nemaline myopathy patients with mutations in the nebulin gene
... SD Cell, Bio-Rad, Hercules, CA, ...(#4002, Cell Signaling, Boston, MA, USA) (1:500 in block- ing solution), washed for 30 min and put in secondary antibody goat anti-rabbit HRP (Dako, Glostrup, Denmark) ...
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Mutation causing congenital myasthenia reveals acetylcholine receptor β/δ subunit interaction essential for assembly
... We now uncover an additional domain essential for AChR assembly by identifying and characterizing the molecular defects that cause a severe CMS associated with marked EP-AChR deficiency. The deficiency arises from 2 ...
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The Duck Hepatitis B Virus Polymerase Is Activated by Its RNA Packaging Signal, ɛ
... DHBV polymerase expressed in yeast VLPs [TYDP], in permeabilized native viral cores [VP], or translated in vitro [DP] was treated with micrococcal nuclease to degrade the endogenous nucl[r] ...
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Synergisitic role of ADP and Ca2+ in diastolic myocardial stiffness
... Paulus, 2013). Nevertheless, the majority of the studies thus far have been based on membrane-permeabilized myocytes, where the relatively low experimental temperature (15ºC) in concert with sarcolemma digestion ...
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