persister cell
Prophages and Growth Dynamics Confound Experimental Results with Antibiotic Tolerant Persister Cells
... coli persister forma- tion through protease Lon and activation of toxin-antitoxin (TA) ...of persister formation in a refined assay setup that uses robust culture conditions and unravels the dynamics of ...
18
Tolerant, Growing Cells from Nutrient Shifts Are Not Persister Cells
... and persister cells and to avoid attributing traits of growing tolerant cells to dormant persister cells, groups should utilize the following ...of persister cells should not increase prior to ...
5
The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation
... that persister cells act as a reservoir for chronic infections [7,8], and it has been shown that a broad range of bacterial species can form persister ...form persister cells at a frequency of ...
15
Reassessing the Role of Type II Toxin Antitoxin Systems in Formation of Escherichia coli Type II Persister Cells
... on the bacterial species, strains, experimental conditions, and antibiotics) combined with the transient nature of persister cells makes them very challenging to study. As a result, the molecular mechanisms ...
14
A Novel Small Molecule Inhibitor of the Mycobacterium tuberculosis Demethylmenaquinone Methyltransferase MenG Is Bactericidal to Both Growing and Nutritionally Deprived Persister Cells
... We developed a novel whole-cell-based screen to detect inhibitors of the respiratory pathway, using induction of PcydAB as a highly specific reporter strain. This assay allowed us to identify DG70 as a potent ...
15
Stochastic Variation in Expression of the Tricarboxylic Acid Cycle Produces Persister Cells
... We constructed mutants lacking representative functional enzymes in early, gltA (citrate synthase) TCA cycle enzymes and in late, gudB (glutamate dehydrogenase), sucA (2-oxoglutarate dehydrogenase), sucC ...
10
Uropathogenic Escherichia coli Metabolite-Dependent Quiescence and Persistence May Explain Antibiotic Tolerance during Urinary Tract Infection
... generating persister cells (11, 47), screening more mini-Tn5 Km mutants may lead to its identifi- ...fewer persister cells than the gnd, zwf, and pykF mutants (Table ...
22
Is bacterial persistence a social trait?
... since persister cells represent an insurance policy that permits population survival in the event of catastrophe ...the persister state could be out- competed by cells which allocate less time to the ...
7
Formation of Persister in Klebsiella pneumoniae: Recombinant Expression and Purification of hipA and hipB proteins from K. pneumoniae
... The cells were grown and lysed to obtain the cell extracts. The cell extracts was centrifuged and the supernatant and pellet were tested for presence of proteins. The recombinant proteins were purified by ...
8
Biofilm infections between Scylla and Charybdis: interplay of host antimicrobial peptides and antibiotics
... of persister cells eliminated by the antibiotic combination with FLIP7, whereas those of the low-BRR antibiotics ciprofloxacin and chloram- phenicol did not reveal persister cells (Figure ...
14
<p>The combination of the glycolysis inhibitor 2-DG and sorafenib can be effective against sorafenib-tolerant persister cancer cells</p>
... of persister cell emergence is therefore critical to designing more effective combina- tion therapies that can achieve a ...HCC cell lines, and the downregulation of HK2 expression can enhance ...
15
Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy
... perturbations. Persister and viable but non-culturable (VBNC) cells are two important clonal bacterial subpopulations that can survive antibiotic ...Both persister and VBNC cells pose a serious threat to ...
12
Sub-Inhibitory Antibiotics Enhance Virulence, Persistence, and Pathogenesis of Uropathogens
... in persister formation, identifying alterations in the hipBA locus as predisposing to persistence (Moyed et ...that persister cells are distinct, and represent a heterogeneous sub-population with unique ...
285
<p>Diosmetin Inhibits Cell Proliferation, Induces Cell Apoptosis and Cell Cycle Arrest in Liver Cancer</p>
... the selectivity to tissue receptors is poor. 9 In recent years, targeted therapy of drugs has attracted attention due to speci fi c receptor proteins and small side effects on normal cells. 1,10,11 Although the occurrence ...
10
Stiffness of Cell Micro-Environment Guides Long Term Cell Growth in Cell Seeded Collagen Microspheres
... of cell micro-environment cannot provide all the necessary signals for full osteogenic commitment of hES-MPs and the role of other factors such as surface topography, porosity, mechanical and biochemical ...
16
Homeobox C10 knockdown suppresses cell proliferation and promotes cell apoptosis in osteosarcoma cells through regulating caspase 3
... Notes: Mg63 cells were randomly divided into four groups: nc group, cells were transfected with an nc; sirna group, cells were transfected with hOXc10-sirna (sirna); ac-DeVD-chO group, cells were transfected with nc and ...
10
TRIM2 downregulation in clear cell renal cell carcinoma affects cell proliferation, migration, and invasion and predicts poor patients’ survival
... RCC is a common and lethal urological malignancy in adults. ccRCC is accounting for ~80% of all RCC histological sub- type and has the highest rate of mortality, invasion, metastasis, and resistance to traditional ...
14
<p>Androgen receptor promotes oral squamous cell carcinoma cell migration by increasing EGFR phosphorylation</p>
... squamous cell carcinoma (OSCC) is a malignant tumor with a high incidence and recurrence rate worldwide and accounts for over 90% of oral cancers, 1 with an annual worldwide incidence of over 300,000 cases and a ...
8
<p>LncRNA LUADT1 Promotes Oral Squamous Cell Carcinoma Cell Proliferation by Regulating miR-34a/GAS1 Axis</p>
... A recent study reported that miR-34a could target GAS1 to promote cell proliferation in papillary thyroid carcinoma. 14 Consistently, our study also showed that miR-34a can downregulate GAS1 to suppress ...
7
Celecoxib suppresses cutaneous squamous-cell carcinoma cell migration via inhibition of SDF1-induced endocytosis of CXCR4
... To investigate the association between celecoxib and CSCC further, we performed a series of studies in human samples and in vitro models to assess the influence of cele- coxib in CSCC-cell migration. Moreover, we ...
9