pH gradient
Human Rhinovirus Type 2 Uncoating at the Plasma Membrane Is Not Affected by a pH Gradient but Is Affected by the Membrane Potential
... Little is known on the driving forces for RNA translocation from the endosome interior into the cytoplasm. The trans- membrane pH gradient and/or the membrane potential may FIG. 2. A pH ...
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Altered pH gradient at the plasma membrane of osteosarcoma cells is a key mechanism of drug resistance
... high pH gradient generated between the acidic extracellular compartment and the alkaline cytosolic space is sufficient to affect the permeability to weak cationic drugs at the plasma membrane ...cytosolic ...
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Optically Driven PH Gradient Generator Based on Self-Assembled Proton Pumps for Activating Hydrogel Microactuators
... driven pH gradient generator provides an alternative non- invasive mechanism for modifying the target solution's acidity without inducing strong electric fields near the solution or exposing the solution to ...
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A pH GRADIENT SAX HPLC METHOD FOR DETERMINATION OF TRACE LEVELS OF OVER SULFATED CHONDROITIN SULFATE AND DERMATAN SULFATE IN HEPARIN SODIUM
... sensitive pH-gradient SAX HPLC method for the determination of Dermatan sulphate (DS) and trace levels of Oversulphated chondroitin sulphate (OSCS) in Heparin sodium was developed and ...A pH ...
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Role of a transbilayer pH gradient in the membrane fusion activity of the influenza virus hemagglutinin: Use of the R18 assay to monitor membrane merging
... low pH is inhibited when the cell cytosol is acidified (3, ...acidic pH in the entry of influenza virus (and possibly other viruses that share the same pathway) into target cells is more subtle, with the ...
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pH gradient stimulated transport of urate and p aminohippurate in dog renal microvillus membrane vesicles
... transmembrane pH gradient (pHo less than pH1) markedly accelerated the uptake of [14C]urate and [3H]PAH and caused the transient accumulation ("overshoot") of each anion above its final level of ...
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Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis
... Methods: We present a method consisting of electrophoresis of complex mixtures of peptides using a strip of filter paper cut into 20 sections laid end to end over a 24-cm-long IPG strip, the pH gradient of ...
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Direct and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures
... directly pH in our dataset, the HMSC models indicated 39 OTUs, of which 17 were Chloroflexi and 8 Proteobacteria (SI ...of pH on plant growth and litter nutrient cycling ...
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THE ROLE OF pH GRADIENT IN THE DISTRIBUTION OF AMMONIA BETWEEN BLOOD AND CEREBROSPINAL FLUID, BRAIN AND MUSCLE
... In an effort to delineate the role of pH in the distribution of ammonia between blood and various body fluids and tissues, temporary pH gradients between blood and cerebrospinal fluid, b[r] ...
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Oxalate transport by anion exchange across rabbit ileal brush border
... K+ gradient and valinomycin, we found no evidence for potential sensitive oxalate ...a pH gradient in the absence of ...Cl gradient (50 mM inside, 5 mM outside) stimulated oxalate uptake ...
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Membrane energization by proton pumps is important for compartmentalization of drugs and toxins: a new type of active transport
... acidic pH is important for a variety of biological reactions, including proteolysis and the invasion of foreign substances ...proton gradient (proton-motive force; pmf) is the concentration of various ...
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Two dimensional Polyacrylamide Gel Electrophoresis pdf
... continuous pH gradient in the polyacrylamide gel ...past, pH gradients were generated by carrier ampholytes or amphoteric buffer moving free- ly within an acrylamide ...
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Capillary Isoelectric Focusing pdf
... shallow pH gradient further enhance ...immobilized pH gradients, ...the gradient is created ‘arti R cially’, outside the electric R eld (whereas in CA-IEF the pH gradient has to ...
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Missing band 7 membrane protein in two patients with high Na, low K erythrocytes
... Two-dimensional electrophoresis nonequilibrium pH gradient electrophoresis in the first dimension combined with SDS gel electrophoresis in the second dimension resolved the 28,000-dalton[r] ...
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The speciation of the proteome
... Cell culture supernatant proteins of Mycobacterium tuberculosis separated by a narrow range pH gradient between pI 4 and 5 in the first dimension and SDS-PAGE in the second dimension.[r] ...
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Manufacturing Man-Made Magnetosomes: High-Throughput In Situ Synthesis of Biomimetic Magnetite Loaded Nanovesicles.
... The pH gradient established across the membrane initiates the transport of iron ions Fe2+, Fe3+ through the pores opened by electroporation.[34] Inside the liposomes, the iron ions react[r] ...
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Live-Cell Imaging and Measurement of Intracellular pH in Filamentous Fungi Using a Genetically Encoded Ratiometric Probe
... cytoplasmic pH gradient or cytoplasmic pH gradient across the apical 20- m region of actively growing ...in pH (⬎0.1 pH unit) across distances of ⬎ 25 ...cytoplasmic ...
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Ion exchange mechanisms on the erythrocyte membrane of the aquatic salamander, Amphiuma tridactylum
... Effects of adrenaline, DIDS and amiloride on the water content, extracellular pH pHe, intracellular pH pH,, and on the pH gradient ApH of Amphiuma tridactylum erythrocytes equilibrated w[r] ...
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Effect of alkylation on the physical properties of simian virus 40 T-antigen species.
... The two-dimensional separation of extracts prepared from infected cells after electrophoresis by non-equilibrium pH gradient electrophoresis A or by equilibrium isoelectric focusing B ar[r] ...
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Isoelectric Focusing pdf
... the pH gradient is d(pH)/dx and d /d(pH) represents the titration curve of the ...a pH gradient is quite dif R cult to maintain, especially as one approaches pH extremes ...
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