Phage display antibody library
Mimotopes of the Vi Antigen of Salmonella enterica Serovar Typhi Identified from Phage Display Peptide Library
... mimotope phage clones were further characterized with a se- ries of ...the phage library is specifically bound to the antibody, two different ELISAs (indirect and sandwich) were carried out to ...
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Screening and selection of peptides specific for esophageal cancer cells from a phage display peptide library
... for phage display selections. However, direct screening of antibody libraries against intact cells allows specific antibodies to be selected under normal physiological conditions ...peptide ...
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Identification Of Dengue-Specific B-Cell Epitopes By Phage-Display Random Peptide Library
... selected phage clones were evaluated with HAF either against the four dengue serotypes, West Nile virus (WNV) or Eastern equine encephalitis virus (EEEV), and a control ascitic fluid (NAF) using an indirect ...
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Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library
... Following immunization of a wobbegong shark (Orec- tolobus ornatus) with three malaria biomarker proteins, the levels of IgNAR response against malaria recombi- nant PfLDH and PfHRP2 significantly increased in the ...
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Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fab phage display library
... Fab phage display library HX01 (Humanyx Pte Ltd, Singa- ...the phage library were separately blocked in 2% skim milk (Sigma) in PBS for 1 h at room tempera- ture ...Pre-blocked ...
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Single Chain Fragment Variables Antibody binding to EGF Receptor in the Surface of MCF7 Breast Cancer Cell Line: Application and Production Review
... of phage-antibodies pool is diversity of species of animal such as human, mice and rates has been demonstrated by Hoogenboom ...However, phage-display libraries of single-library consider less ...
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Original Article Variable domain of the heavy chain of heavy-chain antibody of the Rv0733 antigen of mycobacterium tuberculosis panned and identified from a nonimmune llama VHH phage display library
... by phage enzyme-linked immunosorbent assay and sequencing ...Rv0733-VHH-Fc-6His antibody binding to Rv0733 ...Rv0733-VHH antibody binding with intracellular ...
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Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library
... USA); phage DNA se- quencing was performed by Shanghai Bioengineering Ltd (Shanghai, PR China); unrelated phages were the amplified phages from original phage peptide library; peptide HCBP1 (FQHPSFI) ...
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Generation and Characterization of Monoclonal Antibodies against Dengue Virus Type 1 for Epitope Mapping and Serological Detection by Epitope-Based Peptide Antigens
... on phage makes it easier to identify the antibodies in serum sam- ples from DEN-1-infected ...using phage-displayed serotype-specific epitopes of DEN-1, namely, DA6-7-C4 and DA11-13-C1, were 75% (6/8) and ...
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Screening and identification of a renal carcinoma specific peptide from a phage display peptide library
... USA). Phage DNA sequen- cing was performed by Shanghai Sangon Corp (Shanghai, PR ...anti-rabbit antibody and rabbit anti-M13 bacteriophage antibody were purchased from Pharmacia (Peapack, NJ, ...
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Cloning the Antibody Response in Humans with Chronic Inflammatory Disease: Immunopanning of Subacute Sclerosing Panencephalitis (SSPE) Brain Sections with Antibody Phage Libraries Prepared from SSPE Brain Enriches for Antibody Recognizing Measles Virus Antigens In Situ
... represent antibody directed against the causative ...an antibody phage display library from the brain of a human with subacute sclerosing panencephalitis (SSPE), a chronic encephalitis ...
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Induction of anti-EGFR immune response with mimotopes identified from a phage display peptide library by panitumumab
... monoclonal antibody therapies, including short half-life, the need to repeat administration, inadequate tissue distribution, possible immunogenicity against therapeutic antibodies, the development of resistance, ...
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Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor
... recombinant antibody library construction differed from conventional antibody phage display library assem- bly [10-13], a pre-selection step was introduced to remove ...
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Selection and Evaluation of Specific Single Chain Antibodies against CD90, a Marker for Mesenchymal and Cancer Stem Cells
... A phage antibody display library of scFv was produced as described previously ...helper phage was added and cultures were incubated at 37 ºC for 30 ...
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Phage display library selection of a hypoxia-binding scFv antibody for liver cancer metabolic marker discovery
... Hypoxia, which is frequently observed in liver cancer and metastasis, influences tumor progression and resistance to therapy. Although hypoxia-associated biomarkers are of use in other cancers, none is recognized as a ...
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Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library
... Monoclonal antibodies can detect and inhibit cancer cells through binding to specific antigens on the cell surface. 28 The success of this method depends, to a large extent, on finding an ideal antigen for cancer cell ...
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Preparation and characterization of a single-domain antibody specific for the porcine epidemic diarrhea virus spike protein
... Fig. 2 Schematic diagram of the construction and screening of a single-domain phage display library. Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples of a Bactrian ...
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Novel Human Monoclonal Antibody Combination Effectively Neutralizing Natural Rabies Virus Variants and Individual In Vitro Escape Mutants
... served antigenic site I (19). To find a novel anti-RV MAb that was complementary with CR57, phage display selections using two RV-immune phage antibody libraries were performed. This yielded a ...
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Identification of glycosaminoglycan binding regions in the Plasmodium falciparum encoded placental sequestration ligand, VAR2CSA
... Phage display is a strong and widely used tool for map- ping protein ligand interactions and has in several studies been used to define adhesive parts of proteins present on the surface of different ...
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Application of antibody engineering techniques to develop antibodies for detection of inflammatory and malignant disease
... human library containing 3 x 1 0 ^ clones resulted in the generation o f antibodies to over 25 different antigens, including 'foreign' antigens such as turkey egg white lysosome, bovine serum albumin (Marks et ...
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