phage display peptide library
Screening and selection of peptides specific for esophageal cancer cells from a phage display peptide library
... The Ph.D.-12 TM Phage Display Peptide Library and E. coli ER2738 were purchased from New England Biolabs (Beverly, MA, USA). The Eca109 cell line and human esophageal can- cer tissue sections ...
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Induction of anti-EGFR immune response with mimotopes identified from a phage display peptide library by panitumumab
... a phage display peptide library with ...the phage-derived peptides and EGFR by alignments, both peptides mimic the conformational structure of EGFR binding to ...from phage ...
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Identification of a conserved JEV serocomplex B cell epitope by screening a phage display peptide library with a mAb generated against West Nile virus capsid protein
... Ten phage clones were selected for reactivity with 6D3 following enrichment of the phage display peptide ...ten phage clones that were selected following three rounds of enrichment of ...
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Mimotopes of the Vi Antigen of Salmonella enterica Serovar Typhi Identified from Phage Display Peptide Library
... identify peptide mimotopes of disease-related im- munodominant carbohydrate epitopes from a phage display peptide library by using pooled patient ...of peptide-base diagnostic ...
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Screening and identification of a renal carcinoma specific peptide from a phage display peptide library
... selected phage toward target A498 cells, the phage clone M13 (clone ZT-2) was isolated, amplified and purified for immuno- chemical ...M13 phage and target cells (A498) was evaluated by ...
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Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library
... random peptide phage display library and thus differs from other previously reported phage display library panning strategies ...a phage- binding ...the ...
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Evolution of Cyclic Peptide Scaffolds to Target Nucleic Acids
... Ph.D.-C7C™ Phage Display Peptide Library Kit from New England Biolabs (NEB) with a few ...of phage solution containing ...binding phage were non-specifically eluted with a ...The ...
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Identification of glycosaminoglycan binding regions in the Plasmodium falciparum encoded placental sequestration ligand, VAR2CSA
... Phage display is a strong and widely used tool for map- ping protein ligand interactions and has in several studies been used to define adhesive parts of proteins present on the surface of different ...
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Peptide Ligands to Human Immunodeficiency Virus Type 1 gp120 Identified from Phage Display Libraries
... activity. Peptide 12p1 did not have significant inhibitory activity up to 200 mM in a cell-cell fusion assay, ...The peptide library was screened against soluble monomeric gp120, where- as on the ...
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Selection of Peptide Inhibitor to Matrix Metalloproteinase-2 Using Phage Display and Its Effects on Pancreatic Cancer Cell lines PANC-1 and CFPAC-1
... Phage display is a powerful method to screen in vitro random libraries of peptides and to select short sequences with high affinity and specificity for bio- logical ...12-mer peptide phage ...
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Advancement and applications of peptide phage display technology in biomedical science
... tions. Phage display is a potent and versatile method for studying protein-protein interaction ...a library in which all hu- man SH3 domains are expressed on the surface of M13 bacteriophage, thereby ...
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Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor
... antibody library construction differed from conventional antibody phage display library assem- bly [10-13], a pre-selection step was introduced to remove antibody inserts that were either; ...
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Cognate peptide-receptor ligand mapping by directed phage display
... for 4 minute sat 37°C and the reaction was terminated by adding EDTA to a final concentration of 70 mM. After pre- cipitation, the fragments were treated with 0.001 units mung bean nuclease per µg DNA for 5 minutes at ...
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Monoclonal Antibodies against the Adeno-Associated Virus Type 2 (AAV-2) Capsid: Epitope Mapping and Identification of Capsid Domains Involved in AAV-2–Cell Interaction and Neutralization of AAV-2 Infection
... in peptide competition experiments using Western blot analyses of AAV-2-Ad5-infected ...fragment phage display library (⬘) identified immunopositive clones and sequence analysis of these ...
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Development of a Peptide Mediated Capture PCR for Detection of Mycobacterium avium subsp paratuberculosis in Milk
... on phage display technology, a peptide-mediated magnetic separation technique was developed to facilitate selective isolation of Mycobacterium avium ...commercial phage-peptide ...
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Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library
... continues peptide with 15 residues, ...target peptide in the secondary (B) and three-dimensional structure (C) of the human KDR3 virtualized using visual molecular dynamics (VMD) ...
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Phage display library selection of a hypoxia-binding scFv antibody for liver cancer metabolic marker discovery
... Pyruvate kinase, as a cytoplasmic enzyme, is generally not expected to localize at the cell surface. However, the specific binding of the H103 scFv Ab to hypoxic cells (Figures 2C, 2D, 3B, 4A, 4C; Table S2) and its ...
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Exploration of the HIF-1α/p300 interface using peptide and Adhiron phage display technologies.
... second phage display experiment used non-antibody binding proteins presented on the surface of the phage as opposed to short peptides; such scaffolds are designed to constrain and present variable ...
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Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
... Ydj1 peptide substrate were added to the ...indicates peptide substrate in ...A peptide GLYEIS with no detectable binding affinity to Ydj1 was used as negative ...the peptide GLYEIS only ...
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Identification Of Dengue-Specific B-Cell Epitopes By Phage-Display Random Peptide Library
... selected phage clones were evaluated with HAF either against the four dengue serotypes, West Nile virus (WNV) or Eastern equine encephalitis virus (EEEV), and a control ascitic fluid (NAF) using an indirect ...°C. ...
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