Phase imaging from label-free biological samples
A flexible low cost quantitative phase imaging microscopy system for label free imaging of multi cellular biological samples
... system imaging system and therefore, worsen the lateral ...on phase modulations using an SLM [29] or wire grid polariser based camera used in the dynamic phase microscope ...the phase maps ...
162
Label-free breast histopathology using quantitative phase imaging
... m from the tumor ...derived from 3 features (related to alignment, proximity to epithelium and relative angle) were found to be the best predictors of DSS and ...
125
Label-Free Optical Imaging and Quantitative Algorithms to Assess Living Biological Systems.
... lifetime imaging microscopy (FLIM) ...the samples, and to easily interpret and visualize the FLIM ...summary, label free nonlinear optical molecular imaging and quantitative algorithms ...
156
Visualization and label-free quantification of microfluidic mixing using quantitative phase imaging
... arise from the dilute solution assumption in Fick ’s second law, which is derived based on the premise that the viscosities of the fluids are not significantly ...
7
A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples
... cell imaging in Drosophila and zeb- rafish embryos ...resonance imaging (mMRI) was used to investigate noninvasively and independent of the optical properties mitotic divi- sions inside the opaque early ...
17
Label-free photonics biosensor transducing nano-biological events
... an imaging system of SPR (also known as SPRi or SPR microscopy) was invented in the late 1980’s [79-82], and forged the 2- diamensional SPR chips that we see ...using imaging system from a CCD camera ...
8
Statistical Reconstruction Methods for 3D Imaging of Biological Samples with Electron Microscopy
... rotation from the three parame- ters) to express the free rotation of the target ...Judging from the measurement histories of prelim- inary reconstructions, we selected 30 iterations for the single ...
136
Label free Raman hyperspectral imaging of single cells cultured on polymer substrates
... hyperspectral imaging has been widely used for label-free mapping of biomolecules in cells, these measure- ments require the cells to be cultured on weakly Raman scattering ...in biological ...
13
Label-Free Characterization of Cancer-Activated Fibroblasts Using Infrared Spectroscopic Imaging
... material from which to record data, because cells appear smaller in the 3D matrix, and thus the cytoplasm is much smaller compared with the nucleus of the ...with samples cultured in monolayers, because ...
9
Development of a label-free Raman imaging technique for differentiation of malaria parasite infected from non-infected tissue
... Raman imaging was used to observe the changes to the molecular composition of mice spleen tissue under malarial infection, compared with non-infected ...samples. From analysis of the Raman ...
7
Advanced imaging of tau pathology in Alzheimer Disease: New perspectives from super resolution microscopy and label-free nanoscopy
... super-resolution imaging of monomer elongation, the frequency of elongated fibrils being correlated with the number of seeds present in the patient’s CSF and therefore the stage of the ...
21
Label-free visualization of fruit lignification: Raman molecular imaging of loquat lignified cells
... of biological tissues and ...of biological tissues and cells ...of samples in an unknown way [26] and dyes used to generate image con- trast can modify native cell behavior ...suffer from a ...
16
Blinking Phase-Change Nanocapsules Enable Background-Free Ultrasound Imaging
... ultrasound imaging. However, while microbubbles can be used for molecular imaging, these agents are limited to the vascular space due to their large size (> 1 ...scale, phase changing, blinking ...
11
Laser tweezer controlled solid immersion lens for high resolution imaging in microfluidic and biological samples
... borrowed from the semiconductor ...burgeoning biological fields is an increase in the capability to analyze and manipulate smaller and smaller objects down to the nanometer level of single DNA and protein ...
9
Continuous Grading of Early Fibrosis in NAFLD Using Label-Free Imaging : A Proof-of-Concept Study
... biopsy samples were rep- resentative and most of them had more than 20 portal tracts (a minimum of 6 portal ...scored from 0 –3. The stage of fibrosis was scored from 0–4: (0: no fibrosis, 1: portal ...
14
Imaging of biological samples using UV light determination of fluorescence yield
... of biological samples by imaging is ...of biological tissue will generally be distorted by the presence of absorbers and scatter- ers, and the measurement of uorescence for medical diagnosis ...
116
Label-Free, Flow-Imaging Methods for Determination of Cell Concentration and Viability
... Our investigation shows that FIM techniques have the po- tential to determine total cell concentration and cell viability. This provides a strong basis for more detailed and specific studies in order to validate ...
10
Label-free cell nuclear imaging by Grüneisen relaxation photoacoustic microscopy
... Benefitting from the optical sectioning imaging capability of GRUV-PAM, accurate three-dimensional distributions and internal structures of cell nuclei can be revealed, which are important diagnostic ...
11
Label-Free Luminescent Mesoporous Silica Nanoparticles for Imaging and Drug Delivery
... of the silica matrix (Figure 4c). Instead, we found many ca. 3 nm carbon dots (C-Dots) in the remaining solution (Figure 4c), which we believe are the true luminescence source. Indeed, previous studies with C-Dots showed ...
8
A label-free Electrochemical DNA Biosensor for the Determination of Low Concentrations of Mitoxantrone in Serum Samples
... Received: 10 January 2017 / Accepted: 18 April 2017 / Published: 12 June 2017 Herein, a sensitive, accurate and precise DNA biosensor for the quantification of low levels of mitoxantrone in serum samples was ...
14