Pichia pastoris
Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition (SCADA) Software for Automation of High Cell Density Pichia pastoris Fermentations in a 30L Bioreactor.
... compared to methanol. The glycerol concentration of the batch phase typically starts with 40 g/l. During the glycerol fed-batch phase, glycerol media is continuously supplied to the culture to maintain glycerol ...
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Cloning and constitutive expression of Deschampsia antarctica Cu/Zn superoxide dismutase in Pichia pastoris
... yeast Pichia pastoris to express the SOD because of the advantages it offers when compared to other expression systems, such as a high growth rate at high-cell densities, using a simple culture ...P. ...
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Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris
... and Pichia pastoris should have a high potential of riboflavin formation considering the amount of flavin bound in alcohol oxidase ...P. pastoris does not produce riboflavin when grown on glucose, ...
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Production in Pichia pastoris of complementary protein-based polymers with heterodimer-forming WW and PPxY domains
... The so-called WW domain is found in various natural proteins and binds particular proline-rich peptides with a high degree of specificity [26]. As its name suggests, the amino acid sequence of the WW domain contains two ...
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Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison
... yeast Pichia pastoris, also known as Komagataella phaffii, is widely used for the production of recombinant proteins, due to its high productivity, the ability to grow on defined and inexpensive media and ...
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Perdeuteration of cholesterol for neutron scattering applications using recombinant Pichia pastoris
... Deuteration of biomolecules has a major impact on both quality and scope of neutron scattering experiments. Cholesterol is a major component of mammalian cells, where it plays a critical role in membrane permeability, ...
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Construction of a novel Pichia pastoris strain for production of xanthophylls
... producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β -carotene-pathway enzymes to produce β -carotene, as a vessel for recombinant astaxanthin ...
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Heterologous expression of abaecin peptide from Apis mellifera in Pichia pastoris
... The yeast Pichia pastoris is methylotrophic, which means a capacity of using the methanol as its only car- bon source. The yeast oxidizes the methanol producing formaldehyde and hydrogen peroxide, using ...
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Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris
... to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory ...P. pastoris has emerged a promising host for most of heterologous proteins ...P. Pastoris ...
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Design and Construction of a Sensing and Control System for Methanol Feeding in 30L High Cell Density Pichia pastoris Fermentations.
... as Pichia pastoris compensates for this deficiency by generating large amounts of the ...in Pichia can be regulated by the same cellular mechanism as the regulation of alcohol oxidase (Invitrogen ...
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Functional inclusion bodies produced in the yeast Pichia pastoris
... Pichia pastoris cells were successfully transformed with the plasmid pPM2dZ30-pGAP encoding a VP1GFP ver- sion with a yeast-optimized gene sequence (Additional file ...
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Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha
... yeasts, Pichia pastoris and Hansenula polymorpha were used for the comparative heterologous production of endoglucanase ...P. pastoris and ...than Pichia in higher ...in Pichia than ...
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Improved microscale cultivation of Pichia pastoris for clonal screening
... host Pichia pastoris was applied effectively to support miniaturized phenotyping of clonal libraries in batch as well as fed-batch ...P. pastoris constitutively expressing AppA phytase from ...
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Study on Cellulase Gene Expressed in Pichia pastoris and Analyses of Its Biochemical Characters
... into Pichia pastoris by electroporation. The recombi- nant Pichia pastoris was selected and incubated with YPD medium for cellu- lase ...in Pichia pastoris ...
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Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
... yeast Pichia pastoris is well-known for the production of a broad spectrum of functional types of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein ...
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Construction and Expression of L-Arabinose Isomerase (L-AI) in Cell- Surface of Pichia pastoris
... Abstract— araA gene encode L -arabinose isomerase ( L -AI). It is an enzyme converting D -galactose to D -tagatose. D -tagatose is is a hexoketose monosaccharide sweetener, which is an isomer of D -galactose and rarely ...
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Defined Media Design for High Cell Density Pichia pastoris Fermentations in Shake Flasks, a 24 Well Microreactor and a 30L Bioreactor.
... of Pichia pastoris in shake flasks, a microreactor, and a 30L ...P. pastoris to this media and the preparation of frozen stock cultures were developed, as well as methods for shake flask inoculation ...
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Expression of lignocellulolytic enzymes in Pichia pastoris
... though Pichia pastoris is a good host for the expression of heterologous proteins [3] there is still space for improvements on transcriptional [6,7] and (post-) translational level ...in Pichia ...
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Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
... Insulin was the first recombinant product approved by the FDA for human application [6,7]. The first expression systems were based on the separate expression of the A- and B-chains fused to carrier proteins in two ...
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Genotyping of recombinant Pichia pastoris strains
... of Pichia pastoris offers many advantages that are comparable to those of higher organisms, two of the most important being the ability to perform post-translational modifications and the capacity to yield ...
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