PIGMENT EPITHELIAL-CELLS
Mislocalisation of BEST1 in iPSC-derived retinal pigment epithelial cells from a family with autosomal dominant vitreoretinochoroidopathy (ADVIRC)
... of cells into ...weekly. Cells were allowed to spontaneous differentiate for approximately 6–8 weeks, at which point distinct pigmented colonies could be ...pigmented cells were plated at a density ...
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Regulation of Inwardly Rectifying K Channels in Retinal Pigment Epithelial Cells by Intracellular pH
... The RPE has an intimate anatomical and functional relationship with the adjacent photoreceptor cells. Microvilli project from the apical surface of the RPE and interdigitate with the photoreceptor outer segments, ...
10
miR 194 suppresses epithelial mesenchymal transition of retinal pigment epithelial cells by directly targeting ZEB1
... RPE cells compared to R28 cells, which is a retinal precursor cell line with markers of both glial and neuronal origin, and primary Müller cell lines (Figure ...retinal cells, the primary RPE ...
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Genetic ablation of retinal pigment epithelial cells reveals the adaptive response of the epithelium and impact on photoreceptors
... RPE cells were positive, and an absence of any positive X- gal dye in non-transgene animals (top ...positive cells was found in choroid plexus epithelium of CreER Lac Z transgenics (left) compared to ...
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Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid
... RPE cells from early and mid passages (data shown are derived from passage 6), were cultured on FBS or AF pre-coated glass cover slips in a 24-well microplate (Nunc, Denmark) at a density of 1 × 10 5 cells ...
10
Reactive oxygen intermediates increase vascular endothelial growth factor expression in vitro and in vivo
... retinal pigment epithelial cells exposed to superoxide or hydrogen peroxide rapidly increased VEGF mRNA lev- ...glioblastoma cells with superoxide or hydrogen ...retinal pigment ...
10
Optimization of an in vitro bilayer model for studying the functional interplay between human primary retinal pigment epithelial and choroidal endothelial cells isolated from donor eyes
... retinal pigment epithelial cells (hRPE) and human primary choroidal endothelial cells (hCEC) from donor eyes to construct a bilayer of hCEC/hRPE on transwell ...
7
Original Article Exendin-4 protects retinal pigment epithelial cells against oxidative stress in vitro via PI3K/Akt-STAT3 activation
... RPE cells and attenuated the oxidative stress induced damage, such as decreased cellular viability, and increased cytotoxicity and apoptotic cells ...RPE cells from oxidative stress damage via ...
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Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus
... Short reads were filtered for adapters and reads of low quality using Cutadapt (v.1.8) [8] with error rate of 0.2 and minimum length of 18 bp, aligned against GEN- CODE human genome reference assembly GRCh38. p3 using ...
7
Original Article Crocin protects retinal pigment epithelial cells from oxidative stress through suppression of the MAPK signaling pathway
... leads cells to growth and survival, while activation of p38 MAPK and JNK also plays a critical role in cell growth, senescence, apoptosis and survival [25, ...
7
Original Article Advanced glycation end products induced the epithelial-mesenchymal transition in retinal pigment epithelial cells via ERK activation
... diseases, including diabetic complications, aging and atherosclerosis [10]. Recently, a con- siderable number of studies have suggested that AGEs might play a pivotal role in diabetic retinopathy. Elevated levels of AGEs ...
10
Original Article RKIP inhibits the proliferation and migration of human retinal pigment epithelial cells by suppressing the MAPK pathway
... RPE cells play a key role in diabetic ...these cells is an essential step in the pathogenesis of retinal diseases that lead to vision loss ...RPE cells and their surrounding micro- environment that ...
7
Original Article Role of heat shock protein 47 in epithelial-mesenchymal transition of retinal pigment epithelial cells stimulated by TGF-β2
... Increasing evidence indicates that EMT is a cru- cial pathophysiologic procedure in the develop- ment and progression of PVR, in which RPE cells are the major contributors [13]. Various inflammatory cytokines are ...
7
Gene profiling of human VEGF signaling pathways in human endothelial and retinal pigment epithelial cells after anti VEGF treatment
... RPE cells that were treated with bevacizumab were over-expressed compared to controls (MAPK genes, the SPHK gene and the VEGFA gene), while others (the KDR, NOS, PIK3R and PLA2Ggenes) were under- ...RPE ...
8
Influence of Cell Confluency on the Expression of the α4 Integrin Subunit of Retinal Pigment Epithelial Cells
... by cells from the immune system and its expression by non-hematopoietic cells is ...RPE cells, a result that has been validated by immunofluorescence and FACS ...RPE cells form a confluent ...
10
Original Article EGFL6 regulates hypoxia-induced angiogenic factors in retinal pigment epithelial cells
... retinal pigment epi- thelium (RPE), which forms a blood-retinal bar- rier between the neural retina and choriocapil- laris, is important in maintaining the ocular angiogenic homeostasis ...RPE cells are the ...
7
Directional protein secretion by the retinal pigment epithelium: roles in retinal health and the development of age-related macular degeneration
... a B crystallin is a molecular chaperone induced by stress stimuli. By suppressing protein aggregation and inhibiting the proteolytic activity of caspase 3, it is able to play a role in cytoprotection [58]. a B Crys- ...
11
NLRP3 Inflammasome and Pathobiology in AMD
... The main clinical hallmark and one of the early signs of AMD development is deposition of drusen between the retinal pigment epithelium (RPE) and the underlying choroid. The major constituents of drusen include, ...
21
In Vitro Culture of Shell Gland Epithelial Cells in Japanese Quail (Coturnix coturnix japonica)
... DOI: 10.4236/ojas.2018.82013 185 Open Journal of Animal Sciences surface 3.5-2hours before oviposition [6] [9] [10]. The ovulatory process, possi- bly the preovulatory surge of gonadotropin(s) or progesterone, and ...
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Original Article Plasticity of adult human retinal pigment epithelial cells
... Retinal pigment epithelium (RPE) cell damage underlies many degenerative, dystrophic, and proliferative diseases of the human ...RPE cells can be used for in vitro modeling of RPE cell behavior under ...
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