Plaque reduction neutralization test
Varicella zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique
... A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed usin[r] ...
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Pseudo plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean Congo hemorrhagic fever virus
... by neutralization assay which is one of the most specific serological ...Virus neutralization tests are usually based on the cytopathic effect (CPE) or the plaque-reduction ...
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Potential Application of Nonstructural Protein NS1 Serotype Specific Immunoglobulin G Enzyme Linked Immunosorbent Assay in the Seroepidemiologic Study of Dengue Virus Infection: Correlation of Results with Those of the Plaque Reduction Neutralization Test
... An NS1 serotype-specific indirect enzyme-linked immunosorbent assay (ELISA) was developed to differen- tiate primary and secondary dengue virus infections and serotypes of primary dengue virus infection. For this report, ...
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Plaque reduction neutralization test for human cytomegalovirus based upon enhanced uptake of neutral red by virus infected cells
... It was noted in this laboratory that, within a few days after inoculation, foci of CMV-infected cells stain more intensely with neutral red than do surrounding uninfected cells in the mo[r] ...
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Comparison of commercial enzyme immunoassay kits with plaque reduction neutralization test for detection of measles virus antibody
... EIA test uses microtiter plates with one set of wells coated with the measles virus antigen derived from simian kidney cells and a second set of wells coated with a control antigen derived from uninfected simian ...
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Evaluation of a Novel Reporter Virus Neutralization Test for Serological Diagnosis of Zika and Dengue Virus Infection
... Plaque reduction neutralization test. A standard plaque assay was performed with each patient specimen according to the method described by Shan et ...90% neutralization titers ...
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Use of Sindbis/Eastern Equine Encephalitis Chimeric Viruses in Plaque Reduction Neutralization Tests for Arboviral Disease Diagnostics
... Eastern equine encephalitis virus (EEEV) is a highly virulent, mosquito-borne alphavirus that causes severe and often fatal neurological disease in humans and horses in eastern North American, the Caribbean, and Mexico ...
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Monitoring sheep and Culicoides midges in Montana for evidence of Bunyamwera serogroup virus infection
... by plaque reduction neutralization test using all six BUN serogroup viruses known to occur in the United States: Cache Valley virus (CVV), Lokern virus (LOKV), Main Drain virus (MDV), Northway ...
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Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK 21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization
... We recently reported a simplified plaque reduction neutralization test PRNT adapted to semimicro methods in LLCMK2 cells that was comparable in sensitivity and specificity to the standar[r] ...
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Evaluation of Chimeric Japanese Encephalitis and Dengue Viruses for Use in Diagnostic Plaque Reduction Neutralization Tests
... The plaque reduction neutralization test (PRNT) is a specific serological test used to identify and confirm arbovirus infection in diagnostic laboratories and monitor immunological ...
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Variation in dengue virus plaque reduction neutralization testing: systematic review and pooled analysis
... As titers were abstracted directly from the literature, our results are limited by published data. Titers are more likely to be reported and published if the findings are unusual or significantly different from previous ...
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Mumps Virus Specific Antibody Titers from Pre Vaccine Era Sera: Comparison of the Plaque Reduction Neutralization Assay and Enzyme Immunoassays
... Among the 74 pre-vaccine era serum samples, 47 (64%) were seropositive by PRN (Table 1). Of these 47 PRN-positive samples, 33 tested positive in the Wampole EIA and 32 tested positive in the IBL EIA. Thus, measured ...
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Plaque reduction neutralization antibody test does not accurately predict protection against dengue infection in Ratchaburi cohort, Thailand
... Findings: In a cohort study in Ratchaburi Province, Thailand, 48 subjects with serologically confirmed symptomatic dengue infection were tested for pre-existing dengue neutralizing antibody using PRNT. Nine subjects had ...
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Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system
... Comparison of neutralizing-antibody titers of six serum samples against three influenza virus strains measured by plaque and focus methods and by the method of Frank et al.. Serum sample[r] ...
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Comparison of Passive Haemagglutination and Neutralization Tests to Demonstrate Genital Herpes Virus (HSV2) Antibodies in Pregnant Women
... haemagglutination test has high degree of sensitivity and specificity for detection of HSV antibody in serum ...haemagglutination test and serum neutralization test can be used to identify ...
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Agreement between ELISA and plaque reduction neutralisation assay in detection of respiratory syncytial virus specific antibodies in a birth Cohort from Kilifi, coastal Kenya
... a step in which serum samples were incubated at 56°C in a water bath for 30 minutes to inactivate complement cascade proteins. Each serum sample was repeatedly diluted 2-fold over ten consecutive dilutions and mixed with ...
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A focus reduction neutralization assay for hepatitis C virus neutralizing antibodies
... The plasmid pJFH-1 containing the full-length cDNA of the JFH-1 isolate (which belongs to subtype 2a (GenBank accession no. AB047639)), was a gift from Dr Wakita (Department of Microbiology, Tokyo Metropolitan Insti- ...
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Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains.
... 7. Plaque reduction assay using the two versions of the DNA-binding domain and the N-terminal deletion ...The plaque reduction experiments were performed at 10- and 40-fold molar plasmid ...
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Sensitive plaque neutralization assay for parainfluenza virus types 1, 2, and 3 and respiratory syncytial virus
... Homologous and cross-reactive antibody titers ofparainfluenza virus type I guinea pig serum Cross-reactive titer with following virus strain: Homologous CF titerb Nt test Parainfluenza 1[r] ...
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Effect of an experimental mouthwash extracted from miswak (salvadorapersica) on cariogenic bacteria and dental plaque formation
... the plaque samples collection, a proper reflection and isolation of the working field were performed followed by dryness of the teeth with sterile ...the plaque samples were pooled from many teeth and ...
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