plasma membrane ATPase 1
Two inhibitors of yeast plasma membrane ATPase 1 (ScPma1p): toward the development of novel antifungal therapies
... To further rule out the possibility of general, non-spe- cific inhibition, we considered all 794 PubChem-cata- logued screens that included NSC11668 (August, 2017). To identify screens that used cell-free assays to ...
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Evolutionary and functional analysis of a chara plasma membrane H+-ATPase
... Based on the sequences of three possible Chara PM H + - ATPases contigs (transcript_4956, transcript_1405, transcript_181b) obtained from a sequencing experiment on C. australis RNAs, the most likely open reading frames ...
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Preparation of pure and intact Plasmodium falciparum plasma membrane vesicles and partial characterisation of the plasma membrane ATPase
... Infected erythrocytes were washed twice in 10 volumes of phosphate buffered saline (PBS). The pellet was suspend- ed in 10 volumes of PBS containing 0.05 % (w/v) saponin in order to lyse the erythrocytes. It was then ...
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Saccharomyces cerevisiae Vacuolar H+-ATPase Regulation by Disassembly and Reassembly: One Structure and Multiple Signals
... the plasma membrane. Proton trans- port by plasma membrane V-ATPases in osteoclasts, epididymal clear cells, and renal intercalated cells is necessary for bone resorp- tion, sperm maturation, ...
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Functional expression and segmental localization of rat colonic K adenosine triphosphatase
... in plasma membranes isolated from Sf 9 cells infected with baculovirus containing recombinant DNA with the putative K-ATPase ...K- ATPase activity was not inhibited by ouabain (1 mM); while ...
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Intracellular acidification associated with changes in free cytosolic calcium Evidence for Ca2+/H+ exchange via a plasma membrane Ca(2+) ATPase in vascular smooth muscle cells
... The purpose of this study was to define the mechanism whereby agonists that increase free cytosolic calcium (Cai2+) affect intracellular pH (pHi) in smooth muscle. Rat aortic vascular smooth muscle cells grown on ...
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H(+)V ATPase dependent luminal acidification in the kidney collecting duct and the epididymis/vas deferens: vesicle recycling and transcytotic pathways
... is preferentially expressed in renal intercalated cells and in epididymal proton-secreting cells has a C-terminal DTAL motif (Nelson et al., 1992), which suggests that it is a PDZ domain binding protein. PDZ domains are ...
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Ion Tolerance of Saccharomyces cerevisiae Lacking the Ca2+/CaM-Dependent Phosphatase (Calcineurin) Is Improved by Mutations in URE2 or PMA1
... a plasma membrane Na 1 -ATPase (PMR2/ENA1) is induced by Na 1 or Li 1 Figure ...2 1 /CaM-independent cal- and that full induction requires calcineurin and the cineurin ...
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Proposed Function of the Accumulation of Plasma Membrane-Type Ca2+-ATPase mRNA in Resting Cysts of the Ciliate Sterkiella histriomuscorum
... Phylogenetic analysis. The 35 eukaryotic sequences includ- ed in the analysis comprised 17 SERCAs, 15 PMCAs, and 3 PMR1s. The PMR1s correspond to a third type of Ca 2⫹ - ATPase, which was first identified in S. ...
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Studies of Relationships among Bile Flow, Liver Plasma Membrane NaK ATPase, and Membrane Microviscosity in the Rat
... LPM fluidity, measured by fluorescence polarization using the probe 1,6-diphenyl-1,3,5- hexatriene, was found to be increased by propylene glycol, thyroid hormone, and cortisone acetate, decreased by ethinyl estradiol, ...
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In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis
... on membrane bound enzyme ...the plasma membrane 18 : 2/18 : 1 ratio fol- lowed the inhibition of growth rate, which was influenced not only by the biosynthetic point affected but also by the ...
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Differential expression of genes and proteins between electric organ and skeletal muscle in the mormyrid electric fish Brienomyrus brachyistius
... Electric organs (EOs) have evolved independently in vertebrates six times from skeletal muscle (SM). The transcriptional changes accompanying this developmental transformation are not presently well understood. Mormyrids ...
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A Transgene Encoding a Plasma Membrane H+-ATPase That Confers Acid Resistance in Arabidopsis thaliana Seedlings
... family ( DeWitt and Sussman 1995; DeWitt et al. 1996). The coding regions for two well-characterized 21 amino- acid epitopes from the c-myc mammalian oncogene and the influenza hemagglutinin (HA) proteins ( Kolodziej and ...
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Biogenesis and function of the yeast plasma membrane H(+) ATPase
... Group 1 mutants: defects in protein folding and biogenesis Because some mutations could prevent proper folding or insertion of newly synthesized ATPase into the endoplasmic reticulum membrane, the ...
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Changes in H+ translocating vacuolar type ATPase in the anterior silk gland cell of Bombyx mori during metamorphosis
... The plasma membrane V-ATPase loses its pump activity during the spinning ...(Figs 1, 2). The basal plasma membrane infoldings also showed drastic changes, with large, deep ...
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Kinetics of ATP and Na(+) gradient driven Ca2+ transport in basolateral membranes from gills of freshwater and seawater adapted tilapia
... the membrane fraction by ...This membrane suspension was centrifuged differentially and the basolateral membranes in the resulting supernatant were collected by ...yields membrane suspensions in ...
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Taurocholate transport by rat liver canalicular membrane vesicles Evidence for the presence of an Na+ independent transport system
... canalicular membrane vesicles by a rapid filtration method. The membrane vesicles revealed temperature-dependent, Na+-independent transport of taurocholate into an osmotically reactive intravesicular ...
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Effects of sea water and stanniectomy on branchial Ca(2+) handling and drinking rate in eel (Anguilla anguilla L )
... Sexually immature European eels, Anguilla anguilla L., with a body mass of about 100 g were obtained from a local eel farm (Nijvis B.V., Nijmegen) where they were raised at 25 °C. In the laboratory the fish were kept at ...
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Structure and regulation of insect plasma membrane H(+)V ATPase
... of plasma membrane V-ATPase (Brown and Breton, 1996), which may be maintained in a fully stimulated state since abundant mitochondria deplete oxygen locally and thus establish optimal reducing ...
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Fusicoccin activates pathogen responsive gene expression independently of common resistance signalling pathways, but increases disease symptoms in Pseudomonas syringae infected tomato plants
... the plasma membrane (PM), changes in protein phosphorylation and the production of reactive oxygen species (ROS) (Hammond- Kosack and Jones 1996; Jabs 1997; Ebel and Mithöfer 1998; Blumwald ...
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