Plasmid DNA (pDNA)
Encapsulation of plasmid DNA in calcium phosphate nanoparticles: stem cell uptake and gene transfer efficiency
... encapsulated DNA intact. The TGF- β 1 plasmid was success- fully encapsulated into calcium phosphate nanoparticles with a relatively large loading capacity (the weight ratio of plasmid DNA in ...
15
Catalysis of Henry Reaction by Plasmid DNA
... use plasmid DNA to catalyse the Henry ...of plasmid DNA to catalyse the Henry reaction with high yield but also provides a new option for DNA-based ...that plasmid DNA can ...
11
Replication, Integration, and Packaging of Plasmid DNA following Cotransfection with Baculovirus Viral DNA
... that plasmid sequences had integrated into the viral genome because of ...viral DNA which contained junctions between viral and plasmid ...pUC19 DNA (Fig. 4, lane 10), to release DNA ...
8
Novel approaches to the purification of plasmid DNA for therapeutic application
... of plasmid DNA was considered with a focus on the removal of key contaminants by ...of plasmid DNA for therapeutic applications however is the high-resolution separation of different forms of ...
226
A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery
... good DNA-condensation ability by forming polyplexes with small particle size and positive zeta ...as plasmid DNA vectors to transfect HepG2 in ...
13
Fabrication and characterization of DNA-loaded zein nanospheres
... lating pDNA for gene ...lating DNA were formed using a simple coacervation method, without the use of harsh solvents or tempera- tures, resulting in the preservation of pDNA integrity and tunable ...
13
Genetic manipulation of Staphylococci breaking through the barrier
... transform DNA directly into wild-type staphylococci, the efficiency of plasmid transfer is still low and for some applications borderline for selec- tion ...of plasmid with the methylation pattern ...
9
“Parahomologous” Gene Targeting in Drosophila Cells: An Efficient, Homology-Dependent Pathway of Illegitimate Recombination Near a Target Site
... The small number of plasmid copies integrated into the chromosome in these experiments contrasts with earlier observations of long arrays of plasmid DNA in transformed [r] ...
10
Airway surface liquid contains endogenous DNase activity which can be activated by exogenous magnesium
... a DNA degradation assay in the presence of calcium and magnesium ions ...restricted plasmid DNA (pEGFP-N1,generated by restriction with EcoRI and NotI) by exhaled airway condensate or DNase I ...U/µg ...
5
Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria
... method, Plasmid DNA extraction, profiling and curing, Genomic DNA extraction, Polymerase Chain Reaction (PCR), agarose gel electrophoresis, 16S rRNA gene sequence and phylogenetic analysis were ...
6
Assessing Activity and Inhibition of Middle East Respiratory Syndrome Coronavirus Papain-Like and 3C-Like Proteases Using Luciferase-Based Biosensors
... pMERS-pp3CLpro (for polyprotein containing the nsp5/3CLpro domain). The 3CLpro cleavage site between nsp5 and -6 was either retained, resulting in expression of MERS-3CLpro, which cleaves between nsp4/5 and nsp5/6 and is ...
8
Immunomodulation by mucosal gene transfer using TGF beta DNA
... plasmid DNA. After intranasal administra- tion of TGF- b or vector DNA, different organs including lung, medi- astinal lymph nodes (MLN), spleen, and CLN were removed at inter- ...isolate ...
8
Self-assembled supramolecular nano vesicles for safe and highly efficient gene delivery to solid tumors
... kDa), plasmid DNA condensing efficiency, DNase I degradation protection, and cellular uptake were improved by the supramolecular nano ...the plasmid DNA transferring efficiency in 10% fetal ...
17
Use of multiple markers for investigation of an epidemic of Shigella sonnei infections in Monroe County, New York
... Antibiotic susceptibility patterns, plasmid profiles, and endonuclease restriction analysis of plasmid DNA used in the investigation of an epidemic of Shigella sonnei infections in Monro[r] ...
6
Mutations in Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Zinc Fingers Cause Premature Reverse Transcription
... expression plasmid in an equal molar ratio to generate pseudotyped virus ...VSV-G DNA in our virus preparations for the effectiveness of the DNase I plus subtilisin treatment (see ...the plasmid DNAs ...
11
X4 Human Immunodeficiency Virus Type 1 gp120 Down-Modulates Expression and Immunogenicity of Codelivered Antigens
... GFP plasmid resulted in a low number of apoptotic cells in the injected muscles ...with plasmid DNA encoding the luciferase protein (50 g) and plasmid (50 g) encoding the gp120 (Luc ⫹ ...
10
Regions of incompatibility in single-stranded DNA bacteriophages phi X174 and G4.
... Effect of the G4 reduction sequence on the transduction of single-stranded plasmid particles Plasmid DNA Plasmid DNA in transducing particle Resident plasmid in host cell for the followi[r] ...
8
The Preparation of Preserved Shallot Powders and a Pilot Study of the Antioxidative Effect of Their Aqueous Extracts on the Formation of Hydroxyl Radical Species
... The DNA integrity assay was performed using super- coiled plasmid DNA as the target ...molecule. Plasmid DNA, pGEM-7Zf(-), was transformed into ...the DNA was purified using a ...
7
Novel guanidinylated bioresponsive poly(amidoamine)s designed for short hairpin RNA delivery
... model plasmid DNA to form complexes with these two ...and plasmid DNA complexes were determined with particle sizes less than 90 nm and positive ζ-potentials under 20 mV at nucleic ...
16
Molecular analysis of variant plasmid forms of a bivalent Salmonella typhi Shigella sonnei vaccine strain
... Since restriction fragment analysis had indicated that DNA rearrangements might be occurring in the vaccine plasmid, large plasmid DNA isolated from the clonal variants was digested with[r] ...
6