Plasmid expression and protein production
Construction of pRSET-sfGFP Plasmid for Fusion-Protein Expression, Purification and Detection
... Despite the previously reported low immunogenicity of GFP (Pan et al., 2009), rabbit immunization with expressed and purified sfGFP resulted in a high titer immune response (~1/50000). Fusion to GFP is particularly ...
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Construction, Expression and Preliminary Immunological Evaluation of a DNA Plasmid Encoding the GRA2 Protein of Toxoplasma gondii
... pGRA2 plasmid resulted in production of two protein bands of ≈ 28 and ≈ 24 kDa that were shown to be immunoreactive on Western-blot analysis with GRA2-specific mono- clonal antibody Tg 17-179 ...
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Production of fibronectin binding protein A at the surface of lactococcus lactis increases plasmid transfer In vitro and in vivo
... ml was obtained. GFP detection in enterocytes from the small intestine Strain LL-FnBPA+ containing pValacGFP or LL-FnBPA+ without the expression vector were grown and prepared as described above. Mice were fed by ...
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Construction of an Expression Plasmid (Vector) Encoding Brucella melitensis Outer Membrane Protein, a Candidate for DNA Vaccine
... antigen expression, which can induce and strengthen memory immune responses ...(10-13). Expression of different Brucella antigens, which can induce immune responses, is another important point in DNA ...
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Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid
... the expression cassette PlipB1T and PlipB3T into the expression plasmid pELipAB a ...independent expression cassettes. Each expression cassette contained the complete set of the T7 ...
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Stable Expression Clones and Auto-Induction for Protein Production in E. coli
... 14. Freezer stocks for storage at -70ºC are typically made by placing in a 2-ml plastic freezer tube 1ml of fresh overnight culture grown at 37 ºC in non-inducing MDAG-135, adding 0.1 ml of 80% glycerol, mixing well by ...
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Enhanced membrane protein expression by engineering increased intracellular membrane production
... Additional file 1: Figure S1. Yarrowia lipolytica PAH1 gene and PAH1-knockout generation. A. Domain structure of the Pah1 protein, where the conserved N-lipin domain is shown in green. The asterix represents the ...
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Investigation of mechanisms for enhancing expression of human FVIII in vitro; application to therapeutic protein production
... Insert, and backbone DNAs were ligated using T4 ligase overnight then transformed into Stbl3 E.coli competent cells. Following overnight culture, colonies were selected, grown for 16 hours and then DNA was extracted. In ...
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Construction and Sequencing of Dense Granular14 (GRA14) Gene of Toxoplasma gondii (RH) in Expression Prokaryotic Plasmid PET32a: A Preliminary Study in Vaccine Production
... granule protein 14 (GRA14) gene is one of the excretory- secretary antigens of Toxoplasma which seems to be an appropriate candidate in production of recombinant vaccines and diagnostic ...further ...
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Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production
... TOL plasmid pWW0 to control production of a model protein, ...heterologous protein production in ...ribosomal protein gene was chosen to control yfp ...
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Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum
... the production of two different proteins glu- tathione-S-transferase (GST) and α-amylase was exam- ...high-copy plasmid, we first adopt GST as a small size cytoplasmic protein and also chose ...
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Probing noise in gene expression and protein production
... produce protein populations with different Fano factors F共t兲 = var关x共t兲兴 / 具x共t兲典, where var关x共t兲兴 and 具x共t兲典 are the variance and the mean of the protein concentration x共t兲, respectively ...gene ...
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Expression Systems for Synthetic Spider Silk Protein Production
... synthetic protein is yet to be seen. The synthetic protein may still need to be further processed to ensure purity, either through ammonium sulfate precipitations or ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... total protein yield at the last purification step was approximately 71 mg of Rep 34 per liter of bacterial cul- ...lated protein migrated as a large homogenous band with a molecular weight between 20 and 30 ...
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Unique Features of Aeromonas Plasmid pAC3 and Expression of the Plasmid-Mediated Quinolone Resistance Genes
... the plasmid. The plasmid was isolated by the maxiprepa- ration method as previously described ...purified plasmid was treated with Plasmid-Safe ATP- Dependent DNase (Epicentre, Madison, WI) to ...
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Side effects of chaperone gene co-expression in recombinant protein production
... the production system upon infection of the cells with recombinant baculovirus vectors carrying the corre- sponding ...reporter protein, which also showed increased stability in presence of the bacterial ...
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Arabidopsis Thaliana AXR4 Recombinant Protein Expression for Polyclonal Antibody Production
... the protein content in the samples were determined using the ...AXR4 protein The inclusion bodies obtained from pET28 AXR4 expression were used to immunise AXR4 rabbits because of the high yield of ...
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A Narcissus mosaic viral vector system for protein expression and flavonoid production
... NMV-based expression system for this species would therefore provide an excellent research and biotechnology ...a protein expression system and for secondary metabolite production are explored ...
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Side effects of chaperone gene co-expression in recombinant protein production
... the protein degrada- tion mediated by ...the production system upon infection of the cells with recombinant baculovirus vectors carrying the corre- sponding ...reporter protein, which also showed ...
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Infectious alphavirus production from a simple plasmid transfection
... virus expression plas- mid into cultured mammalian ...stable plasmid DNA may open up the possibility of using these constructs for stable Sindbis virus vaccines that can be launched via plasmid ...
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